A novel fucoidan complex-based functional beverage attenuates oral cancer through inducing apoptosis, G2/M cell cycle arrest and retarding cell migration/invasion

Chen, Pei Hui; Chiang, Pao Chang; Lo, Wen Cheng; Su, Chun Wei; Wu, Ching‐Yuan; Chan, Chun Hao; Wu, Yi; Cheng, Hsin Chung; Deng, Win Ping; Lin, Hsi Kuei; Peng, Bou Yue

doi:10.1016/j.jff.2021.104665

Cited by 15 publications

(6 citation statements)

References 37 publications

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“…Our results revealed that VMW-FC exhibited significant inhibitory effects on cell proliferation, cell viability and colony forming ability in a dose-dependent manner, demonstrating its anti-proliferation characteristics in CRC. This result is in line with our previous study showing inhibited cell growth in oral cancer after VMW-FC treatment, 15 and further supported by other research indicating fucoidan could inhibit the proliferation activity of the HT-29 CRC cell line. 41 Next, VMW-FC was also found significantly effective in inhibiting cell migration in a time- and dose-dependent manner, disclosing its inhibitory property to cancer metastasis.…”

Section: Discussionsupporting

confidence: 92%

“…Previously, we developed an integrative functional cocktail beverage comprising vegetable juice (V), mulberry and fruits (M), spelt wheat (W), and fucoidan complex (FC) as VMW-FC, which exhibited significant inhibitory effects on oral cancer. 15 However, given the critical incidence and mortality rates of CRC worldwide, the effects of VMW-FC on CRC and its related mechanisms remained unclear. Therefore, we aimed to explore the anti-cancer potential of VMW-FC on CRC in both in vitro and in vivo studies.…”

Section: Introductionmentioning

confidence: 99%

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Anti-Colorectal Cancer Effects of Fucoidan Complex-Based Functional Beverage Through Retarding Proliferation, Cell Cycle and Epithelial–Mesenchymal Transition Signaling Pathways

Chan,

Deng,

Peng

et al. 2023

Integr Cancer Ther

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Background: Fucus vesiculosus-derived fucoidan, a multifunctional bioactive polysaccharide sourced from marine organisms, exhibits a wide range of therapeutic properties, including its anti-tumor effects. While previous research has reported on its anti-cancer potential, limited studies have explored its synergistic capabilities when combined with other natural bioactive ingredients. In this current study, we present the development of an integrative functional beverage, denoted as VMW-FC, which is composed of a fucoidan complex (FC) along with a blend of various herbal components, including vegetables (V), mulberries and fruits (M), and spelt wheat (W). Objective: Colorectal cancer (CRC) remains a significant cause of mortality, particularly in metastatic cases. Therefore, the urgent need for novel alternative medicines that comprehensively inhibit CRC persists. In this investigation, we assess the impact of VMW-FC on CRC cell proliferation, cell cycle dynamics, metastasis, in vivo tumorigenesis, and potential side effects. Methods: Cell growth was assessed using MTT and colony formation assays, while metastatic potential was evaluated through wound healing and transwell migration assays. The underlying signaling mechanisms were elucidated through qPCR and western blot analysis. In vivo tumor formation and potential side effects were evaluated using a subcutaneous tumor-bearing NOD/SCID mouse model. Results: Our findings demonstrate that VMW-FC significantly impedes CRC proliferation and migration in a dose- and time-dependent manner. Furthermore, it induces sub-G1 cell cycle arrest and an increase in apoptotic cell populations, as confirmed through flow-cytometric analysis. Notably, VMW-FC also suppresses xenograft tumor growth in NOD/SCID mice without causing renal or hepatic toxicity. Conclusion: The integrative herbal concoction VMW-FC presents a promising approach for inhibiting CRC by slowing proliferation and migration, inducing cell cycle arrest and apoptosis, and suppressing markers associated with proliferation (Ki-67, PCNA, and CDKs) and epithelial-mesenchymal transition (EMT) (Vimentin, N-cadherin, and β-catenin).

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Anti-Colorectal Cancer Effects of Fucoidan Complex-Based Functional Beverage Through Retarding Proliferation, Cell Cycle and Epithelial–Mesenchymal Transition Signaling Pathways

Chan,

Deng,

Peng

et al. 2023

Integr Cancer Ther

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“…Previous studies have reported that the bioactivities, mainly antioxidant and anti-inflammatory activities, of fucoidans from different brown seaweeds are determined by the sulfate content (Jayawardena et al 2019. Several studies have investigated the practical application of brown seaweed fucoidan in food or functional food products and for medical or pharmaceutical purposes (Citkowska et al 2019, Fitton et al 2019, Chen et al 2021. Therefore, in the current study, we investigated the potential anti-inflammatory activity of fucoidans isolated from E. maxima stipe.…”

Section: Discussionmentioning

confidence: 99%

Structural characterization and anti-inflammatory activity of fucoidan isolated from <italic>Ecklonia maxima</italic> stipe

Lee¹,

Nagahawatta²,

Liyanage³

et al. 2022

Algae

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Enzyme-assisted hydrolysis is frequently used as a cost-effective and efficient method to obtain functional ingredients from bioresources. This study involved the enzyme-assisted hydrolyzation and purification of fucoidan from Ecklonia maxima stipe and the investigation of its anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Fucoidans of Viscozyme-assisted hydrolysate from E. maxima (EMSFs) harvested in Jeju, Korea. Structural and chemical characterizations were performed using fourier transform infrared spectroscopy, scanning electron microscope, and monosaccharide analysis. Among fucoidans, EMSF6 was rich in fucose and sulfate and had a similar structural character to commercial fucoidan. EMSF6 showed a strong inhibitory effect on nitric oxide generation in LPS-induced RAW 264.7 cells and significantly decreased the production of LPS-induced pro-inflammatory cytokines, including interleukin-6, interleukin-1β, and tumor necrosis factor α. The anti-inflammatory potential of EMSF6 was mediated through the downregulation of inducible nitric oxide synthase and cyclooxygenase-2 expression. Thus, fucoidans from E. maxima stipe are promising candidates for functional food products.

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“…The fixed HCT116 cells were stained with PI solution at 37 °C for 30 min. Data capturing and analysis were performed using flow cytometry (Beckman Coulter, USA) ( Liu et al, 2012 ; Chen et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Proanthocyanidin oligomers extract from hawthorn mediates cell cycle arrest, apoptosis, and lysosome vacuolation on HCT116 cells

Sun

Wang

Gao

et al. 2022

Current Research in Food Science

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A novel fucoidan complex-based functional beverage attenuates oral cancer through inducing apoptosis, G2/M cell cycle arrest and retarding cell migration/invasion

Cited by 15 publications

References 37 publications

Anti-Colorectal Cancer Effects of Fucoidan Complex-Based Functional Beverage Through Retarding Proliferation, Cell Cycle and Epithelial–Mesenchymal Transition Signaling Pathways

Anti-Colorectal Cancer Effects of Fucoidan Complex-Based Functional Beverage Through Retarding Proliferation, Cell Cycle and Epithelial–Mesenchymal Transition Signaling Pathways

Structural characterization and anti-inflammatory activity of fucoidan isolated from <italic>Ecklonia maxima</italic> stipe

Proanthocyanidin oligomers extract from hawthorn mediates cell cycle arrest, apoptosis, and lysosome vacuolation on HCT116 cells

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