A Novel Genus of Actinobacterial Tectiviridae

Caruso, Steven M.; deCarvalho, Tagide N.; Huynh, Anthony; Morcos, George; Kuo, Nansen; Parsa, Shabnam; Erill, Ivan

doi:10.3390/v11121134

Cited by 22 publications

(17 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacteriophages will often infect several different hosts within the same bacterial genus, and this host range can vary widely among phages within a given genus (Erill and Caruso 2016;Caruso et al 2019;Ross, Ward, and Hyman 2016). As a consequence, it has been postulated that the intragenera host-phage interaction network is nested, with generalist phages infecting multiple hosts and specialist phages infecting particularly susceptible strains (Flores et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Evidence for shared ancestry between Actinobacteria and Firmicutes bacteriophages

Koert

López-Pérez

Mattson

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Bacteriophages are known to display a broad range of host spectra, typically infecting a small set of related bacterial species. The transfer of bacteriophages between more distant clades of bacteria has often been postulated, but remains mostly unaddressed. In this work we leverage the sequencing of novel cluster of phages infecting Streptomyces bacteria and the availability of large numbers of complete phage genomes in public repositories to address this question. Using phylogenetic and comparative genomics methods, we show that several clusters of Actinobacteria-infecting phages are more closely related between them, and with a small group of Firmicutes phages, than with any other Actinobacteriophage lineage. These data indicate that this heterogeneous group of phages shares a common ancestor with well-defined genome structure. Analysis of genomic %GC content shows that these Actinobacteriophages are poorly adapted to their Actinobacteria hosts, suggesting that this phage lineage originated in an ancestor of the Firmicutes, adapted to high %GC content members of this phylum and later migrated to the Actinobacteria.

show abstract

Section: Discussionmentioning

confidence: 99%

Evidence for shared ancestry between Actinobacteria and Firmicutes bacteriophages

Koert

López-Pérez

Mattson

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…To 750 µL of concentrated phage stock, 3.75 µL of nuclease mix (0.25 mg/mL DNase I, 0.25 mg/mL RNase A, 50% glycerol, 150 mM NaCl) was added, gently mixed by inversion, and incubated at 37 °C for 10 min [ 24 ]. After a step of nuclease inactivation, phage DNA was obtained by phenol extraction and ethanol precipitation [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus Betatectivirus

2021

View full text Add to dashboard Cite

Tectiviruses infecting the Bacillus cereus group represent part of the bacterial “plasmid repertoire” as they behave as linear plasmids during their lysogenic cycle. Several novel tectiviruses have been recently found infecting diverse strains belonging the B. cereus lineage. Here, we report and analyze the complete genome sequences of phages Sato and Sole. The linear dsDNA genome of Sato spans 14,852 bp with 32 coding DNA sequences (CDSs), whereas the one of Sole has 14,444 bp comprising 30 CDSs. Both phage genomes contain inverted terminal repeats and no tRNAs. Genomic comparisons and phylogenetic analyses placed these two phages within the genus Betatectivirus in the family Tectiviridae. Additional comparative genomic analyses indicated that the “gene regulation-genome replication” module of phages Sato and Sole is more diverse than previously observed among other fully sequenced betatectiviruses, displaying very low sequence similarities and containing some ORFans. Interestingly, the ssDNA binding protein encoded in this genomic module in phages Sato and Sole has very little amino acid similarity with those of reference betatectiviruses. Phylogenetic analyses showed that both Sato and Sole represent novel tectivirus species, thus we propose to include them as two novel species in the genus Betatectivirus.

show abstract

“…Within this group, B35SSB and betatectiviral orthologs form a highly compact clade within a very diverse group of sequences including Φ29SSB and related viruses from genus Salasvirus and related Picovirinae, along with predicted proteins from genomic and metagenomic projects that were annotated as bacterial but might as well be encoded by unforeseen viruses. Although other tectiviruses are described or suggested to replicate their genome by a protein-primed mechanism [80][81][82], B35SSB would be specific for betatectiviruses and, strikingly, clearly related with highly distant podoviruses. In line with this, viruses from Betatectivirus and Salasvirus genera infect related hosts from Bacillus genus [83], which supports a possible ancient common ancestor for Φ29 and Bam35 SSBs, providing a possible scenario for a gene transfer event.…”

Section: The Biochemical Characterization Of B35ssb Confirmed Some Features Common To Allmentioning

confidence: 98%

Unlimited cooperativity ofBetatectivirusSSB, a novel DNA binding protein related to an atypical group of SSBs from protein-primed replicating bacterial viruses

Lechuga

Kazlauskas

Salas

et al. 2021

Preprint

View full text Add to dashboard Cite

Bam35 and related betatectiviruses are tail-less bacteriophages that prey on members of the Bacillus cereus group. These temperate viruses replicate their linear genome by a protein-primed mechanism. In this work, we have identified and characterized the product of the viral ORF2 as a single-stranded DNA binding protein (hereafter B35SSB). B35SSB binds ssDNA with great preference over dsDNA or RNA in a sequence-independent, highly cooperative manner that results in a non-specific stimulation of DNA replication. We have also identified several aromatic and basic residues, involved in base-stacking and electrostatic interactions, respectively, that are required for effective protein-ssDNA interaction. Although SSBs are essential for DNA replication in all domains of life as well as many viruses, they are very diverse proteins. However, most SSBs share a common structural domain, named OB-fold. Protein-primed viruses could constitute an exception, as no OB-fold DNA binding protein has been reported. Based on databases searches as well as phylogenetic and structural analyses, we showed that B35SSB belongs to a novel and independent group of SSBs. This group contains proteins encoded by protein-primed viral genomes from unrelated viruses, spanning betatectiviruses and Φ29 and close podoviruses, and they share a conserved pattern of secondary structure. Sensitive searches and structural predictions indicate that B35SSB contains a conserved domain resembling a divergent OB-fold, which would constitute the first occurrence of an OB-fold-like domain in a protein-primed genome.

show abstract

A Novel Genus of Actinobacterial Tectiviridae

Cited by 22 publications

References 81 publications

Evidence for shared ancestry between Actinobacteria and Firmicutes bacteriophages

Evidence for shared ancestry between Actinobacteria and Firmicutes bacteriophages

Comparative Genomics of Prophages Sato and Sole Expands the Genetic Diversity Found in the Genus Betatectivirus

Unlimited cooperativity ofBetatectivirusSSB, a novel DNA binding protein related to an atypical group of SSBs from protein-primed replicating bacterial viruses

Contact Info

Product

Resources

About