A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing

Khandeparker, Rakhee; Verma, Preeti; Deobagkar, Deepti D.

doi:10.1016/j.nbt.2011.08.001

Cited by 70 publications

(66 citation statements)

References 48 publications

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“…Xylanases from the marine sources can have some remarkable qualities with respect to stability at high temperature and pH, which needs attention. There are a few studies reported on marine xylanases (6)(7)(8)(9)(10)(11)(12)(13) and it is important to look into more potential xylanases from the marine sources. Recently, the interest in application of endoxylanases in the production of xylooligosaccharides from xylan sources is growing rapidly.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Xylanase from Bacillus tequilensis BT21: Biochemical Characterization and Its Application in Production of Xylobiose from Agricultural Residues

Khandeparker

Parab

Amberkar

2017

Food Technol. Biotechnol.

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Section: Introductionmentioning

confidence: 99%

Recombinant Xylanase from Bacillus tequilensis BT21: Biochemical Characterization and Its Application in Production of Xylobiose from Agricultural Residues

Khandeparker

Parab

Amberkar

2017

Food Technol. Biotechnol.

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“…The halophilic bacteria and enzymes are very interesting (Khandeparker et al 2011). Because the B. cellulosilyticus was isolated from a saline-alkali soil, the effect of NaCl on Xyn10A activity was evaluated.…”

Section: Effects Of Nacl Tolerance On Enzyme Activitymentioning

confidence: 99%

“…Many xylanases have been reported from various microbes, such as bacteria, fungi, and yeasts (Polizeli et al 2005;Dhiman et al 2008;Rao et al 2008;Khandeparker et al 2011;Tenkanen et al 2013). Xylanases originating from the Bacillus strains are intrinsically interesting because of their characteristics, such as alkali and salt tolerance, cold adaptation, or thermostability; they are widely employed in industrial applications (Chang et al 2004;Huang et al 2006;Mamo et al 2006;Ruller et al 2008;Khandeparker et al 2011;Haddar et al 2012;Thomas et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Xylanases originating from the Bacillus strains are intrinsically interesting because of their characteristics, such as alkali and salt tolerance, cold adaptation, or thermostability; they are widely employed in industrial applications (Chang et al 2004;Huang et al 2006;Mamo et al 2006;Ruller et al 2008;Khandeparker et al 2011;Haddar et al 2012;Thomas et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

Sun

Shi

et al. 2016

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A xylanase (Xyn10A) gene from the saline-alkali-tolerant microorganism Bacillus cellulosilyticus DSM 2522 was cloned and expressed in Escherichia coli BL21 (DE3). The open reading frame was composed of 1008 base pairs, and it encoded 335 amino acid residues belonging to glycosyl hydrolase family 10. The optimal temperature and pH of the purified Xyn10A were 40 °C and 8.0, respectively. The Xyn10A was sensitive to heat and showed obvious cold-adapted activity, retaining 38.3%, 55.7%, and 82.9% of the optimal activity at 4, 20, and 30 °C, respectively. Xyn10A also showed a high level of NaCl tolerance. The highest activity was observed with 1.5 M NaCl. The specific enzyme activity of Xyn10A was as much as 163.8 U/mg. Kinetic assays showed that Km, Vmax, and Kcat were 2.56 mg/mL, 202.5 μM/min/mg, and 132.6 /s, respectively. Additionally, the main hydrolysis products using birchwood xylan as substrate were xylobiose, xylotriose, and xylotetraose, as determined by thin layer chromatography analysis. As a cold-adapted and salt-tolerant enzyme, Xyn10A is an ideal candidate for further research and biotechnological applications.

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“…After incubation at 34°C for 7 days, the plates were flooded with 0.1 % congo red solution. The clear zones around colonies indicated qualitative xylanase activity [22].…”

Section: Determination Of Extracellular Xylanase Productionmentioning

confidence: 99%

Comparison of Bacterial Biodiversity and Enzyme Production in Three Hypersaline Lakes; Urmia, Howz-Soltan and Aran-Bidgol

et al. 2014

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This research is a comparative study on the diversity of halophilic bacteria with hydrolytic activities in three significant hypersaline lakes; Urmia in the northwest and Howz-Soltan and Aran-Bidgol in the central desert in Iran. Isolated strains from these saline lakes were found to be halotolerant, moderately and extremely halophilic bacteria. The bacteria in each saline lake were able to produce different hydrolytic enzymes including amylase, protease, lipase, DNase, inulinase, xylanase, carboxy methyl cellulase, pectinase and pullulanase. 188, 302, 91 halophilic strains were isolated from Urmia Lake, Howz-Soltan and Aran-Bidgol playa, respectively. The numbers of Grampositive strains were more than Gram-negatives, and among Gram-positive bacteria; spore-forming bacilli were most abundant. Due to the unique physico-chemical conditions of the lake environments, the hydrolytic activities of isolated strains were significantly different. For instance, isolated strains from Howz-Soltan playa did not produce pectinase, DNase, amylase, lipase and inulinase, while the isolates from Aran-Bidgol playa had a great ability to produce pectinase and DNase. The strains from Urmia Lake were also good producers of DNase but failed to show any chitinase activity. The diversity of halophilic bacteria from the mentioned three saline lakes was also determined using PCR-amplified 16S rRNA followed by phylogenetic analysis of the partial 16S rRNA sequences.

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A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing

Cited by 70 publications

References 48 publications

Recombinant Xylanase from Bacillus tequilensis BT21: Biochemical Characterization and Its Application in Production of Xylobiose from Agricultural Residues

Recombinant Xylanase from Bacillus tequilensis BT21: Biochemical Characterization and Its Application in Production of Xylobiose from Agricultural Residues

Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

Comparison of Bacterial Biodiversity and Enzyme Production in Three Hypersaline Lakes; Urmia, Howz-Soltan and Aran-Bidgol

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