A Novel Herbal Medicine KIOM-MA Exerts an Anti-Inflammatory Effect in LPS-Stimulated RAW 264.7 Macrophage Cells

Abstract

KIOM-MA was recently reported as a novel herbal medicine effective for atopic dermatitis and asthma. In this study, we have demonstrated the inhibitory effect of KIOM-MA on proinflammatory mediator produced in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. KIOM-MA significantly inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as nitric oxide (NO) and prostaglandin E2 (PGE2). Consistent with the inhibitory effect on PGE2, KIOM-MA suppresses the LPS-induced migration of macrophages and gelatinase activity and the expression of matrix metalloprotease-9 (MMP-9) in a dose-dependent manner. Additionally, KIOM-MA showed a strong suppressive effect on the inflammatory cytokines production such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). We also found that KIOM-MA inhibits the activation of nuclear factor-κB (NF-κB) and represses the activity of extracellular signal-regulated kinase (ERK), p38, and c-Jun NH2-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs). Taken together, we elucidated the mechanism of anti-inflammatory effect of KIOM-MA using RAW 264.7 cells stimulated by LPS.

Fermented herbal formula KIOM-MA-128 protects against acute colitis induced by dextran sodium sulfate in mice

“…The preparation of K-M-128 has been previously described [6, 11]. Briefly, the KIOM-MA formula ( Glycyrrhizae Radix , Polygoni Cuspidati Rhizoma , Sophorae Flavescentis Radix , Cnidii Rhizoma , Arctii Fructus , Ginseng Radix Alba , Scrophulariae Radix , Zizyphi Semen , Angelica Gigantis Radix , and Saposhnikovia Radix ) was purchased from the Korea Medical Herbs Association (Yeongcheon, Korea), and the identification was confirmed by Porf.…”

Bovine recombinant lipopolysaccharide binding protein (BRLBP) regulated apoptosis and inflammation response in lipopolysaccharide-challenged bovine mammary epithelial cells (BMEC)

“…Activation of TLR4 signaling by the LPS-LBP complex induces the production of inflammatory cytokines through the activation of NF-B signaling pathway. NF-B, an important regulatory transcription factor, exists as homo-dimeric or hetero-dimeric complexes of p50 and p65 subunits bound to IB and plays a key role in cellular responses to various stimuli (Oh et al, 2012). The transcription factor NF-B can induce the gene expressions for numerous inflammatory mediators, and inhibiting NF-B activity can prevent the production of inflammatory mediators and reduce the damage to the lungs and other organs (Davis, 2000;Fang et al, 2014).…”

Zingiber mioga (Thunb.) Roscoe attenuates allergic asthma induced by ovalbumin challenge

“…Within the MMP family, the MMP-9 gelatinases are specific to the denatured collagens and collagen-IV of the basement membrane (17). MMP-9 is controlled by the inflammatory mediator NO, as well as cytokines and chemokines, and is produced by various types of cells, including inflammatory, endothelial and epithelial cells (18). MMP-9 is associated with airway remodeling and inflammatory cell infiltration into the airways.…”