A novel higher polyhydroxybutyrate producer Halomonas halmophila 18H with unique cell factory attributes

Çelik, Pınar Aytar; Barut, Dilan; Enuh, Blaise Manga; Gover, Kubra Erdogan; Yaman, Belma Nural; Mutlu, Mehmet; Çabuk, Ahmet

doi:10.1016/j.biortech.2023.128669

Cited by 11 publications

(5 citation statements)

References 45 publications

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“…LNSP3E3-1.2 strain. The FT-IR analysis confirmed the production of PHB by strain LNSP3E3-1.2; other studies have also reported members of genus Halomonas as producers of PHAs with spectra similar to those observed in this study [ 66 , 67 ].…”

Section: Discussionsupporting

confidence: 89%

Diversity and Biotechnological Potential of Cultivable Halophilic and Halotolerant Bacteria from the “Los Negritos” Geothermal Area

Guevara-Luna,

Arroyo-Herrera,

Tapia-García

et al. 2024

Microorganisms

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Soil salinization is negatively affecting soils globally, and the spread of this problem is of great concern due to the loss of functions and benefits offered by the soil resource. In the present study, we explored the diversity of halophilic and halotolerant microorganisms in the arable fraction of a sodic–saline soil without agricultural practices and two soils with agricultural practices (one sodic and one saline) near the geothermal area “Los Negritos” in Villamar, Michoacán state. This was achieved through their isolation and molecular identification, as well as the characterization of their potential for the production of metabolites and enzymes of biotechnological interest under saline conditions. Using culture-dependent techniques, 62 halotolerant and moderately halophilic strains belonging to the genera Bacillus, Brachybacterium, Gracilibacillus, Halobacillus, Halomonas, Kocuria, Marinococcus, Nesterenkonia, Oceanobacillus, Planococcus, Priestia, Salibactetium, Salimicrobium, Salinicoccus, Staphylococcus, Terribacillus, and Virgibacillus were isolated. The different strains synthesized hydrolytic enzymes under 15% (w/v) of salts, as well as metabolites with plant-growth-promoting (PGP) characteristics, such as indole acetic acid (IAA), under saline conditions. Furthermore, the production of biopolymers was detected among the strains; members of Bacillus, Halomonas, Staphylococcus, and Salinicoccus showed extracellular polymeric substance (EPS) production, and the strain Halomonas sp. LNSP3E3-1.2 produced polyhydroxybutyrate (PHB) under 10% (w/v) of total salts.

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Section: Discussionsupporting

confidence: 89%

Diversity and Biotechnological Potential of Cultivable Halophilic and Halotolerant Bacteria from the “Los Negritos” Geothermal Area

Guevara-Luna,

Arroyo-Herrera,

Tapia-García

et al. 2024

Microorganisms

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“…In most bacteria, PHB production proceeds in three steps mediated by the genes located in the phaCAB operon [ 42 , 43 ]. These genes encode three enzymes including β-ketothiolase (PhaA), acetoacetyl-CoA reductase (PhaB), and PHA synthase (PhaC) [ 44 , 45 ]. However, alternative genes that can function as phaCAB genes were predicted in Salinicola salarius strain ES021 leading to the construction of distinct PHB and MCL-PHA synthetic pathways (Fig.…”

Section: Resultsmentioning

confidence: 99%

Genetic characterization of a novel Salinicola salarius isolate applied for the bioconversion of agro-industrial wastes into polyhydroxybutyrate

Abdelrahman,

Barakat,

Ahmed

2024

Microb Cell Fact

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Background Polyhydroxybutyrate (PHB) has emerged as a promising eco-friendly alternative to traditional petrochemical-based plastics. In the present study, we isolated and characterized a new strain of Salinicola salarius, a halophilic bacterium, from the New Suez Canal in Egypt and characterized exclusively as a potential PHB producer. Further genome analysis of the isolated strain, ES021, was conducted to identify and elucidate the genes involved in PHB production. Results Different PHB-producing marine bacteria were isolated from the New Suez Canal and characterized as PHB producers. Among the 17 bacterial isolates, Salinicola salarius ES021 strain showed the capability to accumulate the highest amount of PHB. Whole genome analysis was implemented to identify the PHB-related genes in Salinicola salarius ES021 strain. Putative genes were identified that can function as phaCAB genes to produce PHB in this strain. These genes include fadA, fabG, and P3W43_16340 (encoding acyl-CoA thioesterase II) for PHB production from glucose. Additionally, phaJ and fadB were identified as key genes involved in PHB production from fatty acids. Optimization of environmental factors such as shaking rate and incubation temperature, resulted in the highest PHB productivity when growing Salinicola salarius ES021 strain at 30°C on a shaker incubator (110 rpm) for 48 h. To maximize PHB production economically, different raw materials i.e., salted whey and sugarcane molasses were examined as cost-effective carbon sources. The PHB productivity increased two-fold (13.34 g/L) when using molasses (5% sucrose) as a fermentation media. This molasses medium was used to upscale PHB production in a 20 L stirred-tank bioreactor yielding a biomass of 25.12 g/L, and PHB of 12.88 g/L. Furthermore, the produced polymer was confirmed as PHB using Fourier-transform infrared spectroscopy (FTIR), gas chromatography-mass spectroscopy (GC–MS), and nuclear magnetic resonance spectroscopy (NMR) analyses. Conclusions Herein,Salinicola salarius ES021 strain was demonstrated as a robust natural producer of PHB from agro-industrial wastes. The detailed genome characterization of the ES021 strain presented in this study identifies potential PHB-related genes. However, further metabolic engineering is warranted to confirm the gene networks required for PHB production in this strain. Overall, this study contributes to the development of sustainable and cost-effective PHB production strategies.

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“…The reasons for this are unknown in the context of CUBES01, however, often lie with the uptake mechanisms, which in many bacteria can be rather specific to certain sugars [52, 53]. Of the non-sugar substrates, especially glycerol and acetate allowed high growth and PHB yields [54].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of aHalomonasSpecies for Non-Axenic Growth-Associated Production of Bio-Polyesters from Sustainable Feedstocks

Woo,

Averesch,

Berliner

et al. 2024

Preprint

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Biodegradable plastics are urgently needed to replace petroleum-derived polymeric materials and prevent their accumulation in the environment. To this end, we isolated and characterized a halophilic and alkaliphilic bacterium from the Great Salt Lake in Utah. The isolate was identified as aHalomonasspecies and designated "CUBES01". Full-genome sequencing and genomic reconstruction revealed the unique genetic traits and metabolic capabilities of the strain, including the common polyhydroxyalkanoate (PHA) biosynthesis pathway. Fluorescence staining identified intracellular polyester granules that accumulated predominantly during the strain's exponential growth, a feature rarely found among natural PHA producers. CUBES01 was found to metabolize a range of renewable carbon-feedstocks, including glucosamine and acetyl-glucosamine, as well as sucrose, glucose, fructose, and further also glycerol, propionate, and acetate. Depending on the substrate, the strain accumulated up to ~60% of its biomass [dry w/w] in poly(3-hydroxbutyrate), while reaching a doubling time of 1.7 h at 30°C and an optimum osmolarity of 1 M sodium chloride and a pH of 8.8. The physiological preferences of the strain may not only enable long-term aseptic cultivation but can also facilitate the release of intracellular products through osmolysis. Development of a minimal medium also allowed the estimation of maximum PHB production rates, which were projected to exceed 5 gPHB/h. Finally, also the genetic tractability of the strain was assessed in conjugation experiments: two orthogonal plasmid-vectors were stable in the heterologous host, thereby opening the possibility of genetic engineering through the introduction of foreign genes.

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A novel higher polyhydroxybutyrate producer Halomonas halmophila 18H with unique cell factory attributes

Cited by 11 publications

References 45 publications

Diversity and Biotechnological Potential of Cultivable Halophilic and Halotolerant Bacteria from the “Los Negritos” Geothermal Area

Diversity and Biotechnological Potential of Cultivable Halophilic and Halotolerant Bacteria from the “Los Negritos” Geothermal Area

Genetic characterization of a novel Salinicola salarius isolate applied for the bioconversion of agro-industrial wastes into polyhydroxybutyrate

Isolation and Characterization of aHalomonasSpecies for Non-Axenic Growth-Associated Production of Bio-Polyesters from Sustainable Feedstocks

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