A novel highly fluorescent S, N, O co-doped carbon dots for biosensing and bioimaging of copper ions in live cells

Dai, Yanyu; Liu, Zhichao; Bai, Yunfeng; Chen, Zezhong; Qin, Jun; Feng, Feng

doi:10.1039/c8ra09298b

Cited by 20 publications

(10 citation statements)

References 46 publications

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“…into carbon networks and also by enhancing the crystalline nature. 9,27,28 Among these dopants, nitrogen is preferred due to its comparable atomic size with carbon. Furthermore, nitrogen can alter the electronic and optical properties of carbon dots by injecting more electrons into the carbon dot structure, owing to more valence electrons than carbon.…”

Section: ■ Introductionmentioning

confidence: 99%

N-Doped Carbon Dots Synthesized from Ethylene Glycol and β-Alanine for Detection of Cr(VI) and 4-Nitrophenol via Photoluminescence Quenching

Das

2021

ACS Appl. Nano Mater.

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We present here a facile method for nonaqueous synthesis of highly crystalline nitrogen-doped carbon dots (N-CDs) via oligomerization of ethylene glycol while heating with βalanine at 170 °C for 30 min. It exhibited bright bluish photoluminescence (PL) at λ emission = 461 nm, corresponding to a relative PL quantum yield of 14.3% and an average PL lifetime of 7.4 ns. High-resolution transmission electron microscopy of N-CDs revealed systematically self-assembled transverse oligomeric chains of ethylene glycol doped with β-alanine to form hexagonal symmetry, and their crystalline nature was revealed from electron diffraction measurement. The structure and composition of N-CDs are deduced from 13 C nuclear magnetic resonance, highresolution mass spectrometry, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy measurements, which collectively suggested oligomerization of β-alanine-doped ethylene glycol. The N-CDs are then demonstrated as an efficient probe for quantitative detection of Cr(VI) species at different pHs and 4-nitrophenol (4-NP) via PL quenching. The limits of detection (LoD) are deduced from respective linear Stern−Volmer plots, e.g., 1.1 μM for H 2 CrO 4 (at pH 0.9), 2.5 μM for HCrO 4 − (at pH 3.5), and 0.29 μM for CrO 4 2− (at pH 9.0). The detection of Cr(VI) is highly selective against several cationic (e.g.

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Section: ■ Introductionmentioning

confidence: 99%

N-Doped Carbon Dots Synthesized from Ethylene Glycol and β-Alanine for Detection of Cr(VI) and 4-Nitrophenol via Photoluminescence Quenching

Das

2021

ACS Appl. Nano Mater.

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“…The PL peaks data were integrated using a WaveMetric Igor program. The absolute fluorescent quantum yield was determined through the following equation: Φ x = Φ st ( m x / m st ) ( η x 2 / η st 2 ) [ 31 ] where the subscripts st and x denote standard and test samples, respectively, Φ is the fluorescence quantum yield, m is the gradient from the plot of integrated fluorescence versus absorbance, and η is the refractive index of the solvent. The Φ values were determined to be 0.038, 0.028, 0.034, and 0.023 for exfoliation temperatures of 0 °C, 20 °C, 30 °C, and 40 °C, respectively.…”

Section: Resultsmentioning

confidence: 99%

A Size-Controlled Graphene Oxide Materials Obtained by One-Step Electrochemical Exfoliation of Carbon Fiber Cloth for Applications to In Situ Gold Nanoparticle Formation and Electrochemical Sensors—A Preliminary Study

et al. 2022

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A simple, one-step and facile method has been introduced to prepare fluorescent and electrochemically active carbon nanoparticles with single-size distribution and good long-term stability by electrochemical exfoliation of polyacrylonitrile-based carbon fibers in an alkaline solution-phase condition. The preparation condition was systematically optimized by studying the effect of temperature and electrolytes. It has been found that an electrochemical exfoliation reaction carried out at an applied potential of 2 V vs. Ag/AgCl in a phosphate-ion-containing alkaline solution at a temperature of 40 °C is an ideal condition for the preparation of 14 ± 4 nm-sized carbon nanoparticles. Unlike the literature protocols, there are no filtration and membrane dialysis-based off-line sample pretreatments adopted in this work. The as-prepared carbon nanoparticles were characterized by fluorescence, Raman spectrum, transmission electron microscope, and X-ray photoelectron spectroscopic characterization methods. It was found that the carbon–oxygen functional group rich in graphene–oxide quantum dots (GOQDs) such as carbon nanoparticles were formed in this work. A preliminary study relating to simultaneous electrochemical oxidation and the sensing of uric acid and ascorbic acid with well-resolved peaks was demonstrated as a model system to extend the new carbon material for electroanalytical applications. Furthermore, in situ synthesis of 2 nm-sized gold nanoparticles stabilized by GOQDs was presented. The carbon nanoparticles prepared by the direct method in this work have shown good stability over 6 months when stored at room temperature. The electrochemical exfoliation reaction has been found to be highly reproducible and suitable for bulk synthesis of luminescence-effective carbon nanoparticles to facilitate fundamental studies and practical applications.

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“…It has been reported that doped N and S cause CDs to possess heteroatoms in their carbon frameworks, which influences their photoluminescence [29,30,31,32]. For this reason, a freshly prepared colorless PVP and CYS solution was sealed in a Teflon-lined stainless-steel autoclave that was heated at a constant temperature.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Fluorescent Carbon Dots as Selective and Sensitive Probes for Cupric Ions and Cell Imaging

Huang

Lin

et al. 2019

Molecules

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A novel sensing system has been designed for the detection of cupric ions. It is based on the quenched fluorescence signal of carbon dots (CDs), which were carbonized from poly(vinylpyrrolidone) (PVP) and L-Cysteine (CYS). Cupric ions interact with the nitrogen and sulfur atoms on surface of the CDs to form an absorbed complex; this results in strong quenching of the fluorescence of the CDs via a fast metal-to-ligand binding affinity. The synthesized water-soluble CDs also exhibited a quantum yield of 7.6%, with favorable photoluminescent properties and good photostability. The fluorescence intensity of the CDs was very stable in high ionic strength (up to 1.0 M NaCl) and over a wide range of pH levels (2.0–12.0). This facile method can therefore develop a sensor that offers reliable, fast, and selective detection of cupric ions with a detection limit down to 0.15 μM and a linear range from 0.5 to 7.0 μM (R2 = 0.980). The CDs were used for cell imaging, observed that they were low toxicity to Tramp C1 cells and exhibited blue and green and red fluorescence under a fluorescence microscope. In summary, the CDs exhibited excellent fluorescence properties, and could be applied to the selective and sensitive detection of cupric ion and multicolor cell imaging.

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A novel highly fluorescent S, N, O co-doped carbon dots for biosensing and bioimaging of copper ions in live cells

Abstract: In this study, novel highly fluorescent sulfur, nitrogen, and oxygen co-doped carbon dots (S, N, O-CDs) were prepared from m-phenylenediamine and sulfamide by using the hydrothermal method.

Cited by 20 publications

References 46 publications

N-Doped Carbon Dots Synthesized from Ethylene Glycol and β-Alanine for Detection of Cr(VI) and 4-Nitrophenol via Photoluminescence Quenching

N-Doped Carbon Dots Synthesized from Ethylene Glycol and β-Alanine for Detection of Cr(VI) and 4-Nitrophenol via Photoluminescence Quenching

A Size-Controlled Graphene Oxide Materials Obtained by One-Step Electrochemical Exfoliation of Carbon Fiber Cloth for Applications to In Situ Gold Nanoparticle Formation and Electrochemical Sensors—A Preliminary Study

Synthesis of Fluorescent Carbon Dots as Selective and Sensitive Probes for Cupric Ions and Cell Imaging

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