A novel human RasGAP‐like gene that maps within the prostate cancer susceptibility locus at chromosome 1q25

Abstract

We report the molecular cloning of a human cDNA that encodes a molecule having striking homology with Rasspecific GTPase-activating proteins (RasGAPs). Among previously described RasGAPs, the cDNA product is most closely related to Caenorhabditis elegans GAP-2, including a predicted coiled-coil structure near the carboxyl terminus. Expression of the cDNA in Saccharomyces cerevisiae defective in one of two RasGAPs, Ira2, complemented loss of the Ira2 function, indicating that the cDNA product functions as a RasGAP. The RasGAP-like gene is located on the human chromosome 1q25, the locus that appears to contain a hereditary prostate cancer susceptible gene, HPC1.z 1998 Federation of European Biochemical Societies.

Comparative and evolutionary analysis of genes encoding small GTPases and their activating proteins in eukaryotic genomes

“…The alignment from RasGAP representatives showed four highly conserved blocks, blocks 1, 2, 3A, and 3B (Fig. 4A), similar to the previous report (52). All RhoGAPs contained a RhoGAP domain that exhibited three conserved blocks as shown in the alignment of representative domain sequences (Fig.…”

The A2A Adenosine Receptor Is a Dual Coding Gene

“…The overexpression of Lrrn3 promotes the epidermal growth factorinduced phosphorylation of MAPK (48). Rasal2 is highly homologous to the GTPase-activating protein-related domain of Ras GTPase-activating proteins, which regulate the activation of Ras and are involved in the Ras-MAPK pathway (52,56). Rshl1 inhibits the activation of ERK1/2 kinase during cell cycle progression (51).…”

High-Resolution Genomic Profiling of Carboplatin Resistance in Early-Stage Epithelial Ovarian Carcinoma

“…2 ). The 1q25.2 SRO contains the gene RASAL2 , which encodes a protein containing a GAP-related domain [Noto et al, 1998]. The RASAL2 is therefore proposed to be a member of the RASGAPs, which induce activated RAS to inactivate itself.…”