A novel huperzine A-producing endophytic fungus Fusarium sp. Rsp5.2 isolated from Huperzia serrate

Le, Thanh Thi Minh; Hoang, Anh Thi Hong; Nguyen, Nhue P.; Trinh, Ha Thi Thu; Vo, Thuy Thị Bich; Quyền, Đồng Văn

doi:10.1007/s10529-020-02836-x

Cited by 24 publications

(15 citation statements)

References 20 publications

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“…Currently, about 12 fungal genera have been reported to produce huperzine A [15][16][17][42][43][44][45][46][47], of which 7 genera were found in H. serrata in this study. Among them, Cladosporium, Trichoderma, and Fusarium with the relative abundance > 0.1% had the highest distribution in the stems, followed by the leaves and the roots, indicating that the endophytic fungi in the stems and the leaves were more involved in the synthesis of some secondary metabolites than those in the roots.…”

Section: Plos Onementioning

confidence: 60%

Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata

Fan

Miao

et al. 2020

PLoS ONE

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Endophytic fungi play an important role in plant growth. The composition and structure of endophytes vary in different plant tissues, which are specific habitats for endophyte colonization. To analyze the diversity and structural composition of endophytic fungi from toothed clubmoss (Huperzia serrata) that was artificially cultivated for 3 years, we investigated endophytic fungi from the roots, stems and leaves using comparative sequence analysis of the ITS2 region of the fungal rRNA genes sequenced with high-throughput sequencing technology. Seven fungal phyla were identified, and fungal diversity and structure varied across different tissues, with the most distinctive community features found in the roots. A total of 555 operational taxonomic units (OTUs) were detected, and 198 were common to all samples, and 43, 16, 16 OTUs were unique to the root, stem, leaf samples, respectively. Taxonomic classification showed that Ascomycota and Basidiomycota were dominant phyla, and Cladosporium, Oidiodendron, Phyllosticta, Sebacina and Ilyonectria were dominant genera. The relative abundance heat map at the genus level suggested that H. serrata had characteristic endophytic fungal microbiomes. Line discriminant analysis effect size analysis and principal coordinate analysis demonstrated that fungal communities were tissue-type and tissue-site specific. Overall, our study provides new insights into the complex composition of endophytic fungi in H. serrata.

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Section: Plos Onementioning

confidence: 60%

Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata

Fan

Miao

et al. 2020

PLoS ONE

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“…It was found that ginsenoside Rb1, the main active ingredient of Panax Notoginseng, could be deglycosylated to form ginsenoside F2 and compound K by the glucosidase of the endophytes Fusarium sp. YMF1.02670 and YMF1.02193 (Luo et al 2013) (Fig. 5b).…”

Section: Biotransformation Of Terpenoids By Endophytesmentioning

confidence: 89%

Endophytes: the novel sources for plant terpenoid biosynthesis

Chen

Xing

et al. 2021

Appl Microbiol Biotechnol

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Terpenoids are natural compounds predominantly present in plants. They have many pharmaceutical and/or nutritional functions, and have been widely applied in medical, food, and cosmetics industries. Recently, terpenoids have been used in the clinical treatment of COVID-19 due to the good antiviral activities. The increasing demand for terpenoids in international markets poses a serious threat to many plant species. For environmentally sustainable development, microbial cell factories have been utilized as the promising platform to produce terpenoids. Nevertheless, the bioproduction of most terpenoids cannot meet commercial requirements due to the low cost-benefit ratio until now. The biosynthetic potential of endophytes has gained attention in recent decades owing to the continual discovery of endophytes capable of synthesizing plant bioactive compounds. Accordingly, endophytes could be alternative sources of terpenoid-producing strains or terpenoid synthetic genes. In this review, we summarized the research progress describing the main and supporting roles of endophytes in terpenoid biosynthesis and biotransformation, and discussed the current problems and challenges which may prevent the further exploitation. This review will improve our understanding of endophyte resources for terpenoid production in industry in the future. Key points • The mechanisms employed by endophytes in terpenoid synthesis in vivo and in vitro.• Endophytes have the commercial potentials in terpenoid bioproduction and biotransformation.• Synthetic biology and multiomics will improve terpenoid bioproduction in engineered cell factories.

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“…and Phlegmariurus sp. have been demonstrated to produce 13 : Acremonium , ,, Alternaria , Aspergillus , , Blastomyces , Botrytis , Ceriporia , Cladosporium , , Collectotrichum , ,, Fusarium , , Hypoxylon , , Leptosphaeria , Mycoleptodiscus , Paecilomyces , Paraboeremia , Penicillium , ,, Podospora , Shiraia , , Trichoderma , , and Xylarialern…”

Section: Selected Known Alkaloids From Endophytesunclassified

“…In addition to China, surveys of huperzine-producing endophytes from H . serrata have been conducted in two provinces in Vietnam, at Langbiang Mountain in Lam Dong and in Hoang Lien National Park, Lao Cai . Overharvesting of H .…”

Section: Selected Known Alkaloids From Endophytesmentioning

confidence: 99%