A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice

Song, Lihua; Huang, Yuanming; Liu, Guoxing; Li, Xianping; Xiao, Yuchun; Chang, Liu; Zhang, Yue; Li, Jintong; Xu, Jianguo; Lu, Shan; Ren, Zhihong

doi:10.3389/fimmu.2021.828887

Cited by 25 publications

(20 citation statements)

References 45 publications

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“…A. putredinis decreases in both compensated and decompensated liver cirrhosis patients (Shao et al, 2018), and it was discovered to increase with the intakes of cruciferous vegetable in human body (Li et al, 2009). B. dorei has the effect of antiinfluenza by enhancing the expression of earlier interferon, regulating the balance of pro-or anti-inflammatory cytokines, and reconstructing the composition of the gut microbiome (Song et al, 2021). B. dorei can also reduce the production of LPS, and treatment with live B. dorei may help prevent coronary artery disease (Yoshida et al, 2018) and treat obesity (Yoshida et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Gut microbiome is associated with metabolic syndrome accompanied by elevated gamma-glutamyl transpeptidase in men

Sheng

Su²,

Chen³

et al. 2022

Front. Cell. Infect. Microbiol.

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It is predicted that by 2035, metabolic syndrome (MS) will be found in nearly more than half of our adult population, seriously affecting the health of our body. MS is usually accompanied by the occurrence of abnormal liver enzymes, such as elevated gamma-glutamyl transpeptidase (GGT). More and more studies have shown that the gut microbiota is involved in MS; however, the correlation between gut microbiota and MS with elevated GGT has not been studied comprehensively. Especially, there are few reports about its role in the physical examination of the population of men with MS and elevated GGT. By using the whole-genome shotgun sequencing technology, we conducted a genome-wide association study of the gut microbiome in 66 participants diagnosed as having MS accompanied by high levels of GGT (case group) and 66 participants with only MS and normal GGT level (control group). We found that the number of gut microbial species was reduced in participants in the case group compared to that of the control group. The overall microbial composition between the two groups is of significant difference. The gut microbiota in the case group is characterized by increased levels of “harmful bacteria” such as Megamonas hypermegale, Megamonas funiformis, Megamonas unclassified, Klebsiella pneumoniae, and Fusobacterium mortiferum and decreased levels of “beneficial bacteria” such as Faecalibacterium prausnitzii, Eubacterium eligens, Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Bacteroides dorei, and Alistipes putredinis. Moreover, the pathways of POLYAMSYN-PWY, ARG+POLYAMINE-SYN, PWY-6305, and GOLPDLCAT-PWY were also increased in the case group, which may play a role in the elevation of GGT by producing amine, polyamine, putrescine, and endogenous alcohol. Taken together, there are apparent changes in the composition of the gut microbiome in men with MS and abnormal GGT levels, and it is high time to discover specific gut microbiome as a potential therapeutic target in that population. More in-depth studies of relevant mechanism could offer some new methods for the treatment of MS with elevated GGT.

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Section: Discussionmentioning

confidence: 99%

Gut microbiome is associated with metabolic syndrome accompanied by elevated gamma-glutamyl transpeptidase in men

Sheng

Su²,

Chen³

et al. 2022

Front. Cell. Infect. Microbiol.

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“…55 In animal experiments, mice inoculated with B. dorei are protected from influenza infection. 56 It is also negatively correlated with SARS-CoV-2. 57 In this study, B. dorei was detected in the mouth, which is presumed to be responsible for the mobility of microorganisms in the environment.…”

Section: Effects Of J-12 On Oral and Intestinal Microorganismsmentioning

confidence: 95%

“…Bacteroides dorei is a kind of intestinal microorganism isolated from human faecal samples 49 , which can inhibit the growth of Clostridium di cile and reduce the production of LPS and atherosclerosis 50 . In animal experiments, mice inoculated with B. dorei are protected from in uenza infection 51 . It is also negatively correlated with SARS-CoV-2 52 .…”

Section: Effects Of J-12 On Oral and Intestinal Microorganismsmentioning

confidence: 99%

Both live and heat-killed Bifidobacterium animalis J-12 alleviated oral ulcers in LVG golden Syrian hamsters by gavage by directly intervening in the intestinal flora structure

et al. 2023

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“…The blood on the surface was absorbed with filter paper, and the changes in lung organ index were weighed and recorded using an analytical balance. The formulae used in this study were A = B/C*100%, where A represents the lung organ index, B represents the lung weight (mg), and C represents the body weight (g); and D = (E − F)/G*100%, where D represents the inhibition rate of the lung index (%), E represents the average lung index of the infected group, F represents the average lung index of the administration group, and G represents the average lung index of the infected group (Han et al, 2021;Song et al, 2021;Li-Juan et al, 2022;Wei et al, 2022).…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

Intestinal microbiota analysis and network pharmacology reveal the mechanism by which Lianhua Qingwen capsule improves the immune function of mice infected with influenza A virus

Zhu

et al. 2022

Front. Microbiol.

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ObjectiveLianhua Qingwen capsule (LHQW) can attenuate lung injury caused by influenza virus infection. However, it is unclear whether the intestinal microbiota plays a role in LHQW activity in ameliorating viral infectious pneumonia. This study aimed to investigate the role of intestinal microbiota in LHQW activity in ameliorating viral infectious pneumonia and its possible mechanisms.Research design and methodsA mouse model of influenza A viral pneumonia was established by intranasal administration in BALB/c mice. Detection of influenza virus in the lungs, pathological examination of the lungs and small intestine, and biochemical detection of inflammatory indices were performed. The effects of LHQW on intestinal microbiota were evaluated by 16S rRNA gene sequencing. The key components and targets of LHQW were screened via network pharmacology and verified through molecular docking, molecular dynamics simulation, and free binding energy calculations.ResultsBody weight decreased, inflammatory factor levels were disturbed, and the lung and intestinal mucosal barriers were significantly injured in the infected group. The alpha diversity of the intestinal microbiota decreased, and the abundance of Bacteroidetes, Muribaculaceae_unclassified, and Streptococcus decreased significantly. LHQW treatment reduced the viral load in the lungs, rescued body weight and survival, alleviated lung and intestinal mucosal barrier injury, reversed the reduction in the intestinal microbiota alpha diversity, and significantly increased the abundance of Bacteroidetes and Muribaculaceae. Network pharmacological analysis showed that six active herbal medicinal compounds from LHQW could regulate the intestinal microbiota and inhibit the immune-inflammatory response through the Toll-like receptor (TLR) and nuclear factor-κB (NF-κB) signalling pathways in the lungs.ConclusionThese results suggest that LHQW is effective for treating influenza A virus infectious pneumonia, and the mechanism is associated with the regulation of the TLR4/NF-κB signalling pathway in the lungs by restoring intestinal microbiota and repairing the intestinal wall.

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A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice

Cited by 25 publications

References 45 publications

Gut microbiome is associated with metabolic syndrome accompanied by elevated gamma-glutamyl transpeptidase in men

Gut microbiome is associated with metabolic syndrome accompanied by elevated gamma-glutamyl transpeptidase in men

Both live and heat-killed Bifidobacterium animalis J-12 alleviated oral ulcers in LVG golden Syrian hamsters by gavage by directly intervening in the intestinal flora structure

Intestinal microbiota analysis and network pharmacology reveal the mechanism by which Lianhua Qingwen capsule improves the immune function of mice infected with influenza A virus

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