A novel indel within goat casein alpha S1 gene is significantly associated with litter size

Wang, Ke; Yan, Hailong; Xu, Han; Yang, Qing; Zhang, Sihuan; Pan, Chuanying; Chen, Hong; Zhu, Haijing; Liu, Jinwang; Liu, Qu; Lan, Xianyong

doi:10.1016/j.gene.2018.05.119

Cited by 56 publications

(36 citation statements)

References 56 publications

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“…After sequence alignment with the updated reference genome (NC_030813.1), it was found that the polymorphism was an insertion mutation, and the position changed from 6654-6664 to 8045-8055 ( Figure 1B). Hence, we redefined this polymorphism as NC_030813.1: g.8045-8055insTTTCCGTAATG, compared to the previous name (NC_030813.1: g.6654-6664delTTTCCGTAATG) given by Wang et al [1]. Moreover, in this study, the heterozygote type "ID" actually showed three bands on the agarose gel ( Figure 1A), as the top band was a heteroduplex [41,42].…”

Section: Genetic Parameter Of 11-bp Indel Of Csn1s1 In Three Chinese mentioning

confidence: 57%

“…The meat and cashmere dual-purpose SBWC goats were adult females (2-3 years old), and reared in the Shaanbei white cashmere goat breeding farm in Yulin city of Shaanxi Province [1,34]. Ear samples were randomly collected in these populations between 2016 and 2018 [1,34]. These goats were unrelated and healthy.…”

Section: Sample and Data Collectionmentioning

confidence: 99%

“…According to the previous report [1], a pair of primers (F: 5 -GCTGGAAGCAGTTCGTCA-3 ; R: 5 -GGGTTGATAGCCTTGTATGTT-3 ) was synthesized to detect the 11-bp indel within the CSN1S1 gene in all of the goats. The PCR amplification processes used were the same as in the previous study, including the primers, PCR reaction mixture, and amplification condition.…”

Section: Dna Extraction and Indel Genotypingmentioning

confidence: 99%

“…Furthermore, Lan et al found that the polymorphisms in other casein family genes (CSN3 and CSN1S2) had significant positive effects on the litter size of the Xinong Saanen dairy goat [32]. Therefore, we speculated that the CSN1S1 gene may be linked to the BMPR1B gene in goats, and thus, the variations within CSN1S1 might affect the regulation of fecundity [1].…”

mentioning

confidence: 91%

“…As a powerful candidate gene in livestock breeding, our previous study found that an 11-bp indel within the CSN1S1 gene strongly affected the litter size in a larger sample of the Shaanbei white cashmere goat (SBWC) [1]. However, the relationships between this polymorphism and the milk performance and body measurement traits of goats have not yet been studied, to our knowledge.…”

mentioning

confidence: 96%

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An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

Zhang

Wang

Liu

et al. 2019

Animals

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Simple Summary: In the field of animal breeding, selection using molecular genetic markers, such as insertions/deletions (indels), is a novel method to reduce the generation interval and more accurately improve performance. The casein alpha s1 (CSN1S1) gene is one of the major genes regulating the milk performance of mammals, and it is also relevant to body development. Previous studies have shown that an 11-bp indel in this gene was strongly associated with goat litter size, however, its effects on milk performance and body measurement traits have not been studied. In this study, this indel was genotyped in three Chinese goat breeds, and further analysis results revealed that this indel polymorphism was significantly associated with milk performance in dairy goats, as well as affecting the body measurement traits in all three breeds. These findings hint that the 11-bp indel within CSN1S1 gene could be utilized for effectively selecting goats with excellent performances in terms of production and breeding.Abstract: The casein alpha s1 (CSN1S1) gene encodes α-s1 casein, one of the proteins constituting milk, which affects milk performance, as well as improving the absorption of calcium and bone development in mammals. A previous study found that an 11-bp insertion/deletion (indel) of this gene strongly affected litter size in goats. However, to our knowledge, the relationships between this polymorphism and the milk performance and body measurement traits of goats have not been reported. In this paper, the previously identified indel has been recognized in three Chinese goat breeds, namely the Guanzhong dairy goat (GZDG; n = 235), Shaanbei white cashmere goat (SBWC; n = 1092), and Hainan black goat (HNBG; n = 278), and the following three genotypes have been studied for all of the breeds: insertion/insertion (II), deletion/deletion (DD), and insertion/deletion (ID). The allele frequencies analyzed signified that the frequencies of the "D" allele were higher (47.8%-65.5%), similar to the previous report, which indicates that this polymorphism is genetically stable in different goat breeds. Further analysis showed that this indel was markedly associated with milk fat content, total solids content, solids-not-fat content, freezing point depression, and acidity in GZDG (p < 0.05), and also affected different body measurement traits in all three breeds (p < 0.05). The goats with II genotypes had superior milk performance, compared with the others; however, goats with DD genotypes had better body measurement sizes. Hence, it may be necessary to select goats with an II or DD genotype, based on the desired traits, while breeding. Our study Animals 2019, 9, 1114 2 of 11 provides information on the potential impact of the 11-bp indel polymorphism of the CSN1S1 gene for improving the milk performance and body measurement traits in goats.

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Section: Genetic Parameter Of 11-bp Indel Of Csn1s1 In Three Chinese mentioning

confidence: 57%

Section: Sample and Data Collectionmentioning

confidence: 99%

Section: Dna Extraction and Indel Genotypingmentioning

confidence: 99%

mentioning

confidence: 91%

mentioning

confidence: 96%

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An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

Zhang

Wang

Liu

et al. 2019

Animals

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Assocıatıon between growth hormone gene polymorphısm and some growth traıts in Akkaraman and Anatolian Merino sheep

Tanış,

Keskin

2024

Mol Biol Rep

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A genome-wide perspective on the diversity and selection signatures in indigenous goats using 53 K single nucleotide polymorphism array

Zhong¹,

Wang²,

Huang³

et al. 2023

animal

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A novel indel within goat casein alpha S1 gene is significantly associated with litter size

Cited by 56 publications

References 56 publications

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

Assocıatıon between growth hormone gene polymorphısm and some growth traıts in Akkaraman and Anatolian Merino sheep

A genome-wide perspective on the diversity and selection signatures in indigenous goats using 53 K single nucleotide polymorphism array

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A novel indel within goat casein alpha S1 gene is significantly associated with litter size

Cited by 56 publications

References 56 publications

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

Assocıatıon between growth hormone gene polymorphısm and some growth traıts in Akkaraman and Anatolian Merino sheep

A genome-wide perspective on the diversity and selection signatures in indigenous goats using 53 K single nucleotide polymorphism array

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Product

Resources

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A genome-wide perspective on the diversity and selection signatures in indigenous goats using 53 K single nucleotide polymorphism array