A novel invertebrate toll-like receptor is involved in TLR mediated signal pathway of thick shell mussel Mytilus coruscus

Xu, Kang; Zhang, Zhanying; Xu, Zhongtian; Tang, Zurong; Liu, Lianwei; Lu, Zhanhui; Qi, Pengzhi

doi:10.1016/j.dci.2019.03.012

Cited by 19 publications

(6 citation statements)

References 61 publications

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“…Apart from TLRs, known as PRRs, CTLDs are also one of the earliest discovered lectins and the important PRRs in innate immunity . To evaluate the innate immunity of crustaceans, TLRs were usually used for antibacterial activity, while CTLD were used for antiviral activity . According to transcriptome results, the upregulated DEGs of the 9000 IU/kg group were enriched during antigen processing and presentation, and one of the significant clusters was CTLD.…”

Section: Discussionmentioning

confidence: 99%

“…50 To evaluate the innate immunity of crustaceans, TLRs were usually used for antibacterial activity, while CTLD were used for antiviral activity. 51 According to transcriptome results, the upregulated DEGs of the 9000 IU/ kg group were enriched during antigen processing and presentation, and one of the significant clusters was CTLD. Thus, the transcriptome results showed that CTLD might have a synergy effect with TLRs.…”

Section: ■ Discussionmentioning

confidence: 99%

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Impact of Dietary Vitamin D₃ Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor

Liu

Wang

et al. 2021

J. Agric. Food Chem.

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), as an indispensable and fat-soluble nutrient, is associated with skeletal mineralization and health in mammals. However, such associations have not been well studied in economically important crustaceans. Six levels of vit-D 3 with isonitrogenous and isolipidic diets were used to feed Eriocheir sinensis. The range of optimal vit-D 3 requirements is 5685.43−10,000 IU/kg based on growth. The crabs fed 9000 IU/kg vit-D 3 showed the best growth performance. This vit-D 3 dose significantly increased antioxidant capacity in the hepatopancreas and intestine and was optimal for molting and innate immunity via quantitative polymerase chain reaction analysis. Transcriptomics analyses indicate that vit-D 3 could alter protein processing in the endoplasmic reticulum, steroid biosynthesis, and antigen processing and presentation. As shown by the enzyme-linked immunosorbent assay, vit-D 3 could improve vitamin D receptor, retinoic acid receptor, and C-type lectins concentrations. The 1α,25-dihydroxy vit-D 3 content in serum was significantly higher in 3000−9000 IU/kg vit-D 3 . The study suggests that dietary vit-D 3 and its metabolites can regulate molting and innate immunity in crabs.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Impact of Dietary Vitamin D₃ Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor

Liu

Wang

et al. 2021

J. Agric. Food Chem.

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“…A total of 250 mussels were randomly divided into five groups. The immune challenge assay was performed as in our previous study [ 60 ], in a word, the mussels of five treatment groups were injected in the adductor with 100 μL live Vibrio alginolyticus (3 × 10 7 CFU/mL), lipopolysaccharide (LPS) (L3024, Sigma, from Escherichia coli O111: B4, 50 μg/mL), peptidoglycans (PGN) (77140, Sigma, from Staphylococcus aureus , 0.5 mg/mL), glucan (GLU) (G5011, Sigma, from Saccharomyces cerevisiae , 1 mg/mL), or polyinosinic-polycytidylic acid (poly I:C), (P9582, Sigma, 1 mg/mL), respectively. Of these, V. alginolyticus was obtained from the diseased mussels as the pathogenic bacteria and the rest were purchased from Sigma-Aldrich (Shanghai).…”

Section: Methodsmentioning

confidence: 99%

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

et al. 2023

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The inflammatory cytokine interleukin-17 (IL17) plays an important role in innate immunity by binding to its receptors (IL17Rs) to activate immune defense signals. To date, information on members of the IL17 family is still very limited in molluscan species. Here, a novel member of the IL17 family was identified and characterized from thick shell mussel Mytilus coruscus, and this gene was designated as McIL17-1 by predicting structural domains and phylogenetic analysis. McIL17-1 transcripts existed in all examined tissues with high expression levels in gills, hemocytes and digestive glands. After the stimuli of different pathogen associated molecular patterns (PAMPs) for 72 h, transcriptional expression of McIL17-1 was significantly upregulated, except for poly I:C stimulation. Cytoplasm localization of McIL17-1 was shown in HEK293T cells by fluorescence microscopy. Further, in vivo and in vitro assays were performed to evaluate the potential function of McIL17-1 played in immune response. McIL17-1 was either knocked down or overexpressed in vivo through RNA inference (RNAi) and recombinant protein injection, respectively. With the infection of living Vibrio alginolyticus, a high mortality rate was exhibited in the McIL17-1 overexpressed group compared to the control group, while a lower mortality rate was observed in the McIL17-1 knocked down group than control group. In vitro, the flow cytometric analysis showed that the apoptosis rate of McIL17-1 inhibited hemocytes was significantly lower than that of the control group after lipopolysaccharide stimulation. These results collectively suggested that the newly identified IL17 isoform is involved in the inflammatory response to bacterial infection in M. coruscus.

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“…An important activity of vertebrate macrophages is the phagocytic process: the pathogen is phagocytosed and processed by lysosomes, its molecular components exposed on the plasma membrane in the form of antigens presented to the immune system and rapidly detected by immune receptors present in lymphocytes. This, in turn, activates/regulates the transcription of genes involved in innate responses (Xu et al 2019) and metabolic responses linked to innate responses, such as those triggered by nitric oxide (Bayne et al 2001).…”

Section: Macrophages and Dendritic Cellsmentioning

confidence: 99%

Evolution of immune defence responses as incremental layers among Metazoa

Miccoli

Picchietti

Fausto

et al. 2021

The European Zoological Journal

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Recent evidence show that the classical distinction between innate and acquired immunity can no longer be employed to thoroughly characterize immune defenses. As an example, both insects and vertebrates possess non-canonical defense activities that differ from those identified so far; namely, insects display immune memory and mammals show innate-like lymphocytes. At the base of these observations, it can be speculated that multicellular eukaryotes share immune cells deriving from ancestral granular and non-granular immunocytes as well as sets of soluble effectors and signaling immunerelated molecules. On one hand, evolutionary pressure has selected genes coding for group-specific defense molecules; on the other, some cell types and gene products can be presently considered as being enriched with group-specific adaptations deriving from most ancient protostomes and deuterostomes. The immune defenses of more recent extant animal groups could then be seen as overlying layers constituted by both ancient cells retaining broad features for non-self defenses and more specialized cells and immunomodulatory molecules for group-specific capabilities.

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A novel invertebrate toll-like receptor is involved in TLR mediated signal pathway of thick shell mussel Mytilus coruscus

Cited by 19 publications

References 61 publications

Impact of Dietary Vitamin D₃ Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor

Impact of Dietary Vitamin D₃ Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

Evolution of immune defence responses as incremental layers among Metazoa

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A novel invertebrate toll-like receptor is involved in TLR mediated signal pathway of thick shell mussel Mytilus coruscus

Cited by 19 publications

References 61 publications

Impact of Dietary Vitamin D3 Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor

Impact of Dietary Vitamin D3 Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

Evolution of immune defence responses as incremental layers among Metazoa

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Impact of Dietary Vitamin D₃ Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor

Impact of Dietary Vitamin D₃ Supplementation on Growth, Molting, Antioxidant Capability, and Immunity of Juvenile Chinese Mitten Crabs (Eriocheir sinensis) by Metabolites and Vitamin D Receptor