A Novel Isolate of Bean Common Mosaic Virus Isolated from Crownvetch (Securigera varia L. Lassen)

Abstract

Bean common mosaic virus from the genus Potyvirus has a wide range of hosts and a very negative impact on cultivated crops from the genus Phaseolus. The risk of viral infection of economically important crops increases even if the carriers of the virus are related plant species growing on agroecological interfaces. Such plant species have emerged as new hosts for BCMV, usually harboring novel genetic variants of the virus. A novel genetic variant of BCMV was isolated from a symptomatic crownvetch plant, where the presence of this virus was confirmed via Western blot analysis and via amino acid identities in peptide fragments of CI, HC-pro, and CP proteins using the nanoLC-ESI-Q-TOF. The novel BCMV SVK isolate differed from the most genetically similar one in 0.91% of nucleotides and 1.55% of amino acids. The highest number of amino acid substitutions (8.8% of amino acids) was in the P1 protein, followed by CP (2.44% of amino acids). Minor substitutions were in Hc-pro, CI, and Nib proteins. The symptomatic crownvetch plant was confirmed as a new host and carrier of the novel BCMV isolate.

Molecular Characteristics of Bean Common Mosaic Virus Occurring in Inner Mongolia, China

“…It was proposed that recombination was a relatively common process in potyviruses, and recombination was usually parented by isolates from the same geographical region as the recombinant [25,26]. In addition, it was claimed that to a large extent, the diversity in BCMV populations was driven by both host species and geographic location [27,28]. Using RDP4 software, a major parent of BCMV-CJ25 (MK069986) which infected P. vulgaris in Mexico was detected for BCMV-22Huhe; the minor parent, however, was unknown.…”