A Novel Late Embryogenesis Abundant Like Protein Associated with Chilling Stress in Nicotiana tabacum cv. Bright Yellow-2 Cell Suspension Culture

Abstract

Low temperature is one of the major abiotic stresses limiting the productivity and geographical distribution of many important crops. To identify proteins associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 (BY-2) cell suspension culture, we utilized a proteomic approach with two-dimensional electrophoresis to compare proteins from samples of treated with or without chilling treatment at 4°C. One protein specifically more abundant in chilling treated sample was identified and designated as NtLEA7-3. Rapid amplification of cDNA ends gave rise to a full-length NtLEA7-3 cDNA with a complete open reading frame of 1267 bp, encoding a 322 amino acid polypeptide. Homology search and sequence multi-alignment demonstrated that the deduced NtLEA7-3 protein sequence shared a high identity with LEA-like proteins from other plants. Subcellular localization analysis indicated that the NtLEA7-3 was localized exclusively in the nucleus. When the gene was overexpressed in bright yellow-2 cells, the transgenic bright yellow-2 cells show more resistant to chilling stress than the wild-type cells. In addition, transgenic Arabidopsis plants overexpressing the NtLEA7-3 are much more resistant to cold, drought, and salt stresses. Interestingly, the

Insights on Structure and Function of a Late Embryogenesis Abundant Protein from Amaranthus cruentus: An Intrinsically Disordered Protein Involved in Protection against Desiccation, Oxidant Conditions, and Osmotic Stress

“…Transgenic Arabidopsis thaliana plants overexpressing the Nicotiana tabacum NtLEA7-3 gene are much more resistant to cold, drought, and salt stresses (Gai et al, 2011). Tomato LEA25 increases the salt and chilling stress tolerance when overexpressed in yeast (Imai et al, 1996).…”

Overexpression of Late Embryogenesis Abundant 14 enhances Arabidopsis salt stress tolerance

“…Tomato LE25 increases the salt and chilling stress tolerance when overexpressed in yeast [11]. Transgenic Arabidopsis plants overexpressing NtLEA7-3 are much more resistant to cold, drought, and salt stresses [12]. In addition, other LEA proteins, such as PsLEAM from pea, IbLEA14 from sweet potato, D-19 from cotton and Em from wheat, also enhance abiotic stress tolerance [6,[13][14][15].…”

Identification of Winter-Responsive Proteins in Bread Wheat Using Proteomics Analysis and Virus-Induced Gene Silencing (VIGS)

“…Each reaction included 20 l of the products from the diluted RT reactions, 0.4 l of each primer (forward and reverse), 10 l of GoTaq® qPCR Master Mix (Perfect Real Time; Promega, Madison), and 7.2 l of nuclease-free water. The reactions were incubated in a 96-well plate at 95°C for 3 min, followed by 40 20) under different abiotic stresses. Some seedlings were transferred to an illuminated incubator at 4°C or 42°C for low or hightemperature stress treatments, respectively.…”

“…Examining proteome alterations for proteins is crucial; these proteins, unlike transcripts, are direct sources of plant stress responses (16). The effect of cold on protein abundance has been studied in many plants, such as soybean, barley, wheat, Arabidopsis thaliana, rice, and tobacco (4,(17)(18)(19)(20)(21).…”