A novel layer-structured scaffold with large pore sizes suitable for 3D cell culture prepared by near-field electrospinning

He, Fang; Li, Dawei; He, Jinguang; Liu, Yangyang; Ahmad, Fiaz; Liu, Yali; Deng, Xudong; Ye, Ya-Jing; Yin, Da‐Chuan

doi:10.1016/j.msec.2017.12.016

Cited by 90 publications

(53 citation statements)

References 47 publications

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“…Moreover, the pure cross‐linked gelatin scaffold showed the most calcium deposition. It was reported that the higher hydrophilicity of the scaffold would favor the cell attachment and proliferation (He et al, ). Our previous work reported that the scaffold with gelatin/zein weight ratio of 1/1 had the most hydrophobic surface, while the pure gelatin scaffold showed an instant wetting surface.…”

Section: Discussionmentioning

confidence: 99%

“…There are various methods to fabricate porous scaffolds, including freeze drying, three‐dimensional printing and electrospinning (He et al, ; Woodard, Kmetz, Roth, Page, & Grunlan, ; A. T. Wu et al, ). Compared with the traditional fibers, the electrospun nanofibers have many advantages, such as high porosity, high surface‐to‐volume ratio, and ultrafine and oriented structures (J. Wu & Hong, ).…”

Section: Introductionmentioning

confidence: 99%

“…For tissue engineering, the structure of the nanofibrous scaffolds architecturally mimic the extracellular matrix (ECM), which is beneficial for cell attachment, proliferation and migration (J. Wu & Hong, ). Various polymers have been electrospun for bone generation in vitro (He et al, ; Ren, Wang, Sun, Yue, & Zhang, ; Salifu, Lekakou, & Labeed, ), while only a few studies conducted the in vivo experiments (Oryan, Alidadi, Bigham‐Sadegh, & Moshiri, ; Yao et al, ).…”

Section: Introductionmentioning

confidence: 99%

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In vitro and in vivo assessment of glucose cross‐linked gelatin/zein nanofibrous scaffolds for cranial bone defects regeneration

Deng

Zhang

2019

J Biomed Mater Res

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The purpose of this study was to evaluate the glucose cross‐linked gelatin/zein scaffolds for bone regeneration in vitro and in vivo. The nanofibrous scaffolds exhibited fast mineralization in the concentrated simulated body fluid with the deposited octacalcium phosphate and dicalcium phosphate dehydrate. The nanofibrous scaffolds exhibited no cytotoxic effect on MC3T3e1 cells in a CCK‐8 test. Additionally, scanning electron microscope and confocal laser scanning microscopy images revealed that all the scaffolds were biocompatible and showed excellent support for MC3T3e1 cells. In the osteogenesis characterizations, Alizarin Red staining experiments indicated the improved calcium deposits on the cross‐linked scaffolds, while the alkaline phosphatase activity showed no difference. Furthermore, the in vivo cranial bone regeneration results suggested that the cross‐linked gelatin/zein scaffolds presented a strong positive effect on the cranial bone regeneration with the increased new bone volume and connective tissue formation, but the incorporation of zein in the gelatin scaffolds did not favor the bone regeneration. Moreover, the cross‐linked gelatin scaffold retarded the bone resorption as indicated by the higher levels of IFN‐γ and lower levels of IL‐6, which restricted the differentiation of osteoclasts.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro and in vivo assessment of glucose cross‐linked gelatin/zein nanofibrous scaffolds for cranial bone defects regeneration

Deng

Zhang

2019

J Biomed Mater Res

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“…Modified electrospinning setups can also be utilized for 3D structure such as electrospinning with external heat source [ 38 ] and dual nozzle extrusion system to improve cell infiltration rate. He et al increased the pore size of scaffolds by near-field electrospinning [ 63 ]. Levorson et al also increased cellular infiltration with multiscale scaffold, which contains microfibers and nanofibers evenly distributed throughout the entire construct [ 44 ].…”

Section: Literature Reviewmentioning

confidence: 99%

Recent Progress of Fabrication of Cell Scaffold by Electrospinning Technique for Articular Cartilage Tissue Engineering

Zhou

Chyu

Zumwalt

2018

International Journal of Biomaterials

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As a versatile nanofiber manufacturing technique, electrospinning has been widely employed for the fabrication of tissue engineering scaffolds. Since the structure of natural extracellular matrices varies substantially in different tissues, there has been growing awareness of the fact that the hierarchical 3D structure of scaffolds may affect intercellular interactions, material transportation, fluid flow, environmental stimulation, and so forth. Physical blending of the synthetic and natural polymers to form composite materials better mimics the composition and mechanical properties of natural tissues. Scaffolds with element gradient, such as growth factor gradient, have demonstrated good potentials to promote heterogeneous cell growth and differentiation. Compared to 2D scaffolds with limited thicknesses, 3D scaffolds have superior cell differentiation and development rate. The objective of this review paper is to review and discuss the recent trends of electrospinning strategies for cartilage tissue engineering, particularly the biomimetic, gradient, and 3D scaffolds, along with future prospects of potential clinical applications.

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“…[ 9 ] Beyond simple lines of nanofiber, precise printing of a continuous nanofiber was realized for complex patterns in microscale, by matching the jet impact speed and the collector speed. [ 10 ] Direct‐printing ability of NFES has led the utilization of functional nanofiber to various applications including electronics, [ 11 ] scaffolds for tissue engineering, [ 12 ] optoelectronics, [ 13 ] fiber membrane for a fluidic channel, [ 14 ] sensors, [ 15 ] and energy harvesting. [ 16 ] As described, NFES has achieved remarkable advancement as one of the direct‐printing methods since its first demonstration in 2006.…”

Section: Introductionmentioning

confidence: 99%

Direct‐Printing of Functional Nanofibers on 3D Surfaces Using Self‐Aligning Nanojet in Near‐Field Electrospinning

Shin

Choi

Kim

et al. 2020

Adv Materials Technologies

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This paper reports a high‐resolution, template‐free, and direct‐printing method of functional nanofiber on 3D surfaces using a self‐aligning nanojet (SA‐N) in near‐field electrospinning (NFES). In the lowest regime of NFES, the cone‐jet transition is induced by the surface current, which leads to a unique jetting configuration where the microscale Taylor cone (microcone) is formed on the surface of the spherical‐shape droplet. The microcone rapidly develops to the nanoscale jet where the tangential electric force dominates the kinematics of the charged jet. The spherical‐shape ejection boundary allows the jetting angle from 0° to ±90° in both convex and concave surfaces, enabling precise deposition of nanofiber regardless of the curvature of the 3D surfaces. Using SA‐N, precise printing of functional nanofiber is successfully demonstrated on various 3D geometries, including convex, concave, and inner surface of the 3D structure. The direct‐printing ability of nanofiber on 3D surfaces using SA‐N will be a promising strategy to utilize various functional polymers in flexible electronics, printed electronics, optics, and biomedical engineering.

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A novel layer-structured scaffold with large pore sizes suitable for 3D cell culture prepared by near-field electrospinning

Cited by 90 publications

References 47 publications

In vitro and in vivo assessment of glucose cross‐linked gelatin/zein nanofibrous scaffolds for cranial bone defects regeneration

In vitro and in vivo assessment of glucose cross‐linked gelatin/zein nanofibrous scaffolds for cranial bone defects regeneration

Recent Progress of Fabrication of Cell Scaffold by Electrospinning Technique for Articular Cartilage Tissue Engineering

Direct‐Printing of Functional Nanofibers on 3D Surfaces Using Self‐Aligning Nanojet in Near‐Field Electrospinning

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