A novel LCMSMS method for quantitative measurement of short-chain fatty acids in human stool derivatized with 12 C- and 13 C-labelled aniline

Chan, James Chun Yip; Kioh, Dorinda Yan Qin; Yap, Gaik Chin; Lee, Bee Wah; Chan, Eric Chun Yong

doi:10.1016/j.jpba.2017.01.044

Cited by 81 publications

(62 citation statements)

References 28 publications

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“…In some reported methods, the wet weights of the fecal samples were 2 g, 0.25 g, and 0.05 g . However, as human fecal samples always contain undigested particulate residues, to collect a relative representative sample, the wet weight of the fecal sample was chosen as 3 g in our method.…”

Section: Resultsmentioning

confidence: 99%

A rapid and convenient derivatization method for quantitation of short‐chain fatty acids in human feces by ultra‐performance liquid chromatography/tandem mass spectrometry

Zhang

Huang

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale: Short-chain fatty acids (SCFAs) are associated with intestinal microbiota and diseases in humans. SCFAs have a low response in mass spectrometry, and in order to increase sensitivity, reduce sample consumption, shorten analysis time, and simplify sample preparation steps, a derivatization method was developed. Methods:We converted seven SCFAs into amide derivatives with 4-aminomethylquinoline. The reaction occurred for 20 min at room temperature. The analytes were separated on a reversed-phase C18 column and quantitated in the positive ion electrospray ionization mode using multiple reaction monitoring. Acetic acid-d 4 was used as the stable-isotope-labeled surrogate analyte for acetic acid in the working solutions, while the other stable-isotope-labeled standards were used as internal standards (ISs). Results:Method validation showed that the intra-day and inter-day precision of quantitation for the seven SCFAs over the whole concentration range was ≤3.8% (n = 6). The quantitation accuracy ranged from 85.5% to 104.3% (n = 6). Most important, the collected feces were vortexed immediately with ethanol. Conclusions:This study provides a new derivatization method for a precise, accurate, and rapid quantitation of SCFAs in human feces using ultra-performance liquid chromatography/tandem mass spectrometry. This method successfully determined the concentration of SCFAs in human feces and could assist in the exploration of intestinal microbiota and diseases.

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Section: Resultsmentioning

confidence: 99%

A rapid and convenient derivatization method for quantitation of short‐chain fatty acids in human feces by ultra‐performance liquid chromatography/tandem mass spectrometry

Zhang

Huang

et al. 2020

Rapid Comm Mass Spectrometry

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“…There are an increasing number of detection methods for SCFAs and quantification is getting more precise. Although SCFAs analytical methods have improved a lot in the past years, gas chromatography (GC) is still the most widely used quantification method of fecal SCFAs and its accuracy and rapidity cannot be surpassed by others [19]. Before the SCFAs analysis, the sample should be pretreated.…”

Section: Introductionmentioning

confidence: 99%

The propionic acid and butyric acid in serum but not in feces are increased in patients with diarrhea-predominant irritable bowel syndrome

Tian

Zhuang

Luo

et al. 2019

Preprint

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Background & Aims ： Short-chain fatty acids (SCFAs) play a pivotal role in maintaining homeostasis in humans. It might be involved in the pathogenesis of irritable bowel syndrome (IBS). Our study aims to explore the alteration of SCFAs in patients with diarrhea-predominant IBS (IBS-D). Methods： We recruited patients with IBS-D defined by Rome Ⅳ criteria and age-and-gender matched healthy controls (HCs). A headspace solid-phase microextraction gas chromatography–mass spectrometric (HS-SPME-GC-MS) method was developed for the analysis of acetic, propionic and butyric acid in feces and serum. Results: Compared with HCs, the levels of the serum propionate (2.957 ± 0.157 vs 2.843 ± 0.098 mmol/L, P = 0.012) and butyrate (2.798 ± 0.126 vs 2.697 ± 0.077 mmol/L, P = 0.012) were significantly higher in IBS-D group. No significant differences were found among two groups with regard to the concentration of fecal acetate (4.953 ± 1.065 vs 4.774 ± 1.465 mg/g, P = 0.679), propionate (6.342 ± 1.005 vs 6.282 ± 1.077 mg/g, P = 0.868) and butyrate (2.984 ± 0.512 vs 3.071 ± 0.447 mg/g, P = 0.607). Conclusions ： Metabolites of gut microbiota, the propionic and butyric acid, are increased in patients with IBS-D in serum but not in feces. It suggests that propionic and butyric acid might be involved in the pathogenesis of IBS.

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“…Its effect was reduced by a quenching procedure for excess aniline using a succinic acid/2-mercaptoethanol mixture at 4 C. This approach was utilized to establish the relative abundances of acetic, propionic, and butyric acid during the first 12 months of age (Chan, Kioh, Yap, Lee, & Chan, 2017). Apart from reagents dedicated for biological applications, N-…”

Section: Amines Reagentsmentioning

confidence: 99%

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

El-Maghrabey

Kishikawa

Kuroda

2020

Biomedical Chromatography

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Currently, LC–MS has various applications in different areas such as metabolomics, pharmacokinetics, and pathological studies. Yet, matrix effects resulting from co‐existing constituents remain a major problem for LC–MS [or LC–tandem mass spectrometry (LC–MS/MS)]. Moreover, technical problems and instrumental drifts may lead to ion abundance variance. Thus, an internal standard (IS) is required to guarantee the accuracy and precision of the method. Because of their limited number, isotope‐coded derivatization (ICD) has been recently introduced to overcome this problem. For ICD, a stable heavy isotope‐coded moiety is used for labeling the standard or the control sample and the formed products can act as ISs. A light form of the reagent is used for labeling the sample. Then, both are mixed and analyzed by LC–MS(/MS). This strategy permits the identification of different unknown analytes including potential metabolites and disease biomarkers. All these attributes lead to persistent growth in the applications of ICD LC–MS(/MS) in various biomedical branches. In this article we review the ICD methods published in the last eight years for biomedical applications as well as briefly summarize other applications for environmental and food analyses as some of their used ICD reagents were further applied for analyzing biological specimens or have the potential for that.

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A novel LCMSMS method for quantitative measurement of short-chain fatty acids in human stool derivatized with 12 C- and 13 C-labelled aniline

Cited by 81 publications

References 28 publications

A rapid and convenient derivatization method for quantitation of short‐chain fatty acids in human feces by ultra‐performance liquid chromatography/tandem mass spectrometry

A rapid and convenient derivatization method for quantitation of short‐chain fatty acids in human feces by ultra‐performance liquid chromatography/tandem mass spectrometry

The propionic acid and butyric acid in serum but not in feces are increased in patients with diarrhea-predominant irritable bowel syndrome

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

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