A Novel LncRNA, AC091729.7 Promotes Sinonasal Squamous Cell Carcinomas Proliferation and Invasion Through Binding SRSF2

Abstract

Long non-coding RNAs (lncRNAs) play important roles in various biological progresses of carcinogenesis. However, the function of lncRNAs in human sinonasal squamous cell carcinoma (SNSCC) remains greatly unclear. In the current study, lncRNA AC091729.7 expression was examined in SNSCC samples by using microarray, RNA in situ hybridization (ISH) and real-time fluorescence quantitative PCR (qRT-PCR). Cell viability, colony-formation, wound-healing, and transwell assays were applied to SNSCC cells. Xenograft mouse models were employed to evaluate the role of AC091729.7 in growth of SNSCC in vivo. Human protein microarray (Huprot TM Protoarray) and RNA immunoprecipitation (RIP) were used for identifying AC091729.7 binding proteins in SNSCC. Results showed AC091729.7 was upregulated and closely connected with the survival of the SNSCC patients. Knockdown of AC091729.7 suppressed SNSCC cell migration, proliferation, invasion in vitro. Furthermore, downregulation of AC091729.7 could inhibit the growth of SNSCC in vivo. Moreover, Human protein microarray and RIP suggested that AC091729.7 directly combine with the serine/arginine rich splicing factor 2 (SRSF2). Our results suggest that in the cell progression of SNSCC, lncRNA AC091729.7 plays a carcinogenic role and serves as a novel biomarker and latent curative target in SNSCC patients.

Identification and Validation of a Novel Genomic Instability-Associated Long Non-Coding RNA Prognostic Signature in Head and Neck Squamous Cell Carcinoma

“…A careful literature search revealed that AC023310.4 which located on chromosome 15q11.2 was first reported here, and its biological function has not been reported to date. AC091729.1 is located on chromosome 7, Yu et al identified another version AC091729,7 plays a carcinogenic role and serves as a novel biomarker and latent curative target in sinonasal squamous cell carcinoma patients ( Yu et al, 2019 ). LINC01564 is located on chromosome 6p12.1, Zhang et al reported that LINC01564 was associated with hepatocellular carcinoma cell survival.…”

The role of long non-coding RNAs in the pathogenesis of head and neck squamous cell carcinoma

“… 73 Using RNA immunoprecipitation (RIP), lncRNA AC091729.7 has been found to directly combine with the serine/arginine-rich splicing factor 2 and promote HNSCC cell proliferation, migration and invasion, and tumor growth in vivo. 74 LncRNA MIR31HG targets HIF-1α and p21, promoting cell-cycle progression and inhibiting cell apoptosis, thus facilitating HNSCC cell proliferation and tumorigenesis. 75 Moreover, lncRNA DANCR is responsible for NPC metastasis and the hypoxia phenotype.…”

Splicing factor SRSF2-centric gene regulation

“…In another study, we investigated the role of SRSF2 in T cell exhaustion during tumorigenesis and demonstrated that SRSF2 expression is significantly elevated in exhausted T cells, and that SRSF2 knockdown reverses the exhaustion of T cells by epigenetically regulating the expression of multiple immune checkpoint molecules [6]. In addition, an increasing number of studies have recently shown that SRSF2 gene mutation and dysregulated SRSF2 expression are significantly associated with acute myeloid leukemia (AML) [16,17], myelodysplastic syndromes [18][19][20], systemic mastocytosis [21,22], chronic myelomonocytic leukemia [23][24][25], lung carcinoma [26,27], hepatocellular carcinoma [28], and sinonasal squamous cell carcinoma [29]. These findings indicate that SRSF2 functions as a promising therapeutic target for various diseases.…”