The role of the functional diameter is very important to the thread fitting property; however, the main feature of the thread is the spiral surface in space, which has many parameters and is difficult to measure. To obtain a thread functional diameter more in line with the definition of the standard, an approach based on a compliant mechanism theory is presented for the design of a synthetic measurement probe, and a multi-degree of freedom (DOF) movement of the sensitive probing element is realized. High stiffness and high flexibility of the probe are achieved in the undesired direction and direction of freedom, respectively. A method in which the error source is analyzed is adopted to evaluate the measurement uncertainty. The expanded uncertainty of the thread functional diameter measurement is 0.008 mm (k = 2), which is due to the probe mechanism error motions.