A novel method for the quantification of bovine colostral immunoglobulin G using infrared spectroscopy

Elsohaby, Ibrahim; McClure, J. Trenton; Hou, Siyuan; Riley, Christopher B.; Shaw, R. Anthony; Keefe, Gregory P.

doi:10.1016/j.idairyj.2015.08.004

Cited by 12 publications

(26 citation statements)

References 45 publications

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“…For the IR spectroscopy, the cut‐point of 50 g/L provided strong specificity and moderate sensitivity values across the unheated and heat‐treated colostrum (Table ). The accuracy of the IR spectroscopy (91.7%) to differentiate between unheated poor and good quality colostrum was higher than that reported in previous studies . However, the accuracy of the IR instrument to correctly determine quality of heat‐treated colostrum was lower with more samples with poor IgG levels categorized as adequate.…”

Section: Discussionmentioning

confidence: 56%

“…Collected spectra were converted into a printable format (PRN) in GRAMS software . A previously developed PLS model built for the prediction of bovine colostral IgG concentrations by IR spectra was used to predict colostral IgG concentrations by MATLAB software . The IgG concentration was predicted from each spectrum, and subsequently, the IgG concentration for each colostrum sample was calculated as the average of the 6 replicate values.…”

Section: Methodsmentioning

confidence: 99%

“…The accuracy of the IR spectroscopy (91.7%) to differentiate between unheated poor and good quality colostrum was higher than that reported in previous studies. 11,12,37 However, the accuracy of the IR instrument to correctly determine quality of heat-treated colostrum was lower with more samples with poor IgG levels categorized as adequate. For Brix refractometers, the cut-point 22% Brix showed strong sensitivity and moderate specificity values across the unheated and heat-treated colostrum.…”

Section: Assessment Of Heat-treated Colostrum Qualitymentioning

confidence: 99%

“…Historically, radial immunodiffusion (RID) assay has been the gold standard, direct, quantitative method for measuring colostral IgG concentration and assessing colostrum quality . Recent studies have reported measuring colostral IgG concentration by ELISA; midinfrared spectroscopy; and near‐infrared spectroscopy techniques. Other indirect methods have been developed through correlating IgG concentration to specific gravity and refractive index …”

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confidence: 99%

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Effect of Heat‐treatment on Accuracy of Infrared Spectroscopy and Digital and Optical Brix Refractometers for Measuring Immunoglobulin G Concentration in Bovine Colostrum

Elsohaby

McClure

Dow

et al. 2017

Veterinary Internal Medicne

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BackgroundHeat‐treatment of colostrum is a method developed to reduce calf exposure to pathogens. Infrared (IR) spectroscopy and Brix refractometers can be used for measuring colostral IgG concentration and assessing colostrum quality.ObjectivesTo determine the impact of heat‐treatment on accuracy of IR spectroscopy and Brix refractometers for measuring colostral IgG concentration and assessing colostrum quality before and after heat‐treatment.AnimalsA total of 60 Holstein dairy cows on 8 commercial dairy farms.MethodsA cross‐sectional study was designed to determine the effect of heat‐treatment at 60°C and 63°C each for 30 and 60 minutes duration on colostral IgG concentration measured by the reference radial immunodiffusion (RID) assay, IR spectroscopy, and digital and optical refractometers.ResultsColostrum IgG concentration significantly decreased after heat‐treatment at 63°C for 30 or 60 minutes as measured by RID, but the IgG values remained unchanged when measured by IR spectroscopy and refractometers. The lowest correlation coefficient found between IR spectroscopy (r = 0.70) and RID results was in colostrum heat‐treated at 63°C for 60 minutes. For digital (r = 0.48) and optical (r = 0.50) refractometers, the lowest correlation coefficient was at 63°C for 30 minutes when compared to RID. The accuracy of the IR spectroscopy, digital and optical Brix refractometers was decreased from 91.7 to 80%, 81.7 to 45%, and 80 to 45%, respectively, when colostrum heat‐treated at 63°C for 60 minutes.Conclusions and Clinical ImportanceRadial immunodiffusion, IR spectroscopy, and Brix refractometers exhibit utility for measuring IgG concentration when colostrum heat‐treated at 60°C but does not detect decrease IgG concentrations when heat‐treated at 63°C.

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Section: Discussionmentioning

confidence: 56%

Section: Methodsmentioning

confidence: 99%

Section: Assessment Of Heat-treated Colostrum Qualitymentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of Heat‐treatment on Accuracy of Infrared Spectroscopy and Digital and Optical Brix Refractometers for Measuring Immunoglobulin G Concentration in Bovine Colostrum

Elsohaby

McClure

Dow

et al. 2017

Veterinary Internal Medicne

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“…Current research also deals with the risk factors associated with insufficient passive transfer, mainly poor colostrum quality (Beam et al, 2009;Abdel-Salam et al, 2014;Cabral et al, 2016;Elsohaby et al, 2016;Reschke et al, 2017), inconvenient management factors (e.g. Vogels et al, 2013, Cuttance et al, 2017 and additional risk factors, for example breed, herd size, housing system, month of calving, duration of gestation, dry period length, parity of the dam or individual farm influence (McGuirk and Collins, 2004;MacFarlane et al, 2015;Reschke et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Failure of Passive Transfer of Immunity in Dairy Calves in the Czech Republic

Staněk

Nejedlá

Fleischer

et al. 2019

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Prevalence of failure of passive transfer (FPT) of immunity remains relatively high worldwide.The aim of this study was to estimate the FPT prevalence in Czech dairy calves and to evaluate the selected factors -breed, herd size, sex of calves, single versus twin births and the influence of the season of birth. A total of 1,175 serum samples were taken from calves of Czech Fleckvieh and Holstein breed from 33 herds between October 2015 and October 2017. Serum IgG concentration was determined by reference method for IgG determination -radial immunodiffusion. Statistical evaluation was performed by Kruskal-Wallis test. The concentration of IgG ranged from 1.5 to 46.6 g / L with average value 13.7 g / L and was significantly influenced by breed, size of the herd and season. Using the criterion IgG < 10 g / L, it was found that 34.6 % of calves had FPT. The prevalence of FPT by breed was 42.9 % vs. 24.2 % (Czech Fleckvieh vs. Holstein), by size of the herd 45. 0, 44.4, 25.5 and 22.0 % (< 200, 200 - 399, 400 - 599 and ≥ 600 cows per herd, respectively) and by season 25. 3, 34.6, 29.9 and 52.5 % (spring, summer, autumn and winter, respectively). The sex of calves was not found to be a statistically significant factor. The study in newborn calves showed that FPT is still an important problem in Czech dairy herds, especially in the Czech Fleckvieh breed. In smaller herds and especially in the winter, the prevalence of FPT was very high.

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Effectiveness of visible – Near infrared spectroscopy coupled with simulated annealing partial least squares analysis to predict immunoglobulins G, A, and M concentration in bovine colostrum

et al. 2022

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A novel method for the quantification of bovine colostral immunoglobulin G using infrared spectroscopy

Cited by 12 publications

References 45 publications

Effect of Heat‐treatment on Accuracy of Infrared Spectroscopy and Digital and Optical Brix Refractometers for Measuring Immunoglobulin G Concentration in Bovine Colostrum

Effect of Heat‐treatment on Accuracy of Infrared Spectroscopy and Digital and Optical Brix Refractometers for Measuring Immunoglobulin G Concentration in Bovine Colostrum

Prevalence of Failure of Passive Transfer of Immunity in Dairy Calves in the Czech Republic

Effectiveness of visible – Near infrared spectroscopy coupled with simulated annealing partial least squares analysis to predict immunoglobulins G, A, and M concentration in bovine colostrum

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