A novel method for the functionalization of γ-irradiated single wall carbon nanotubes with DNA

Abstract

In this work we describe a novel method for highly efficient functionalization of single wall carbon nanotubes (SWCNTs) by DNA wrapping. Exposure of SWCNTs to gamma-irradiation (50 kGy) has lowered by one order of magnitude the amount of single stranded deoxyribonucleic acid (ssDNA) required for SWCNT modification. The resulting hybrids of gamma-irradiated SWCNTs and ssDNA were characterized by optical absorbance spectroscopy, Raman spectroscopy and Fourier transform infrared spectroscopy. Atomic force microscopy was used to investigate the morphology of hybrids. While gamma-irradiation in three different media has significantly improved the process of SWCNT dispersion, irradiation in ammonia was the most efficient. The gamma-irradiated SWCNTs functionalized with ssDNA were stabilized by electrostatic forces. This preliminary study suggests that gamma-irradiation can significantly improve the functionalization of SWCNTs with DNA.

In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes

“…A stable water suspension of graphene nanoparticles was prepared by aqueousphase exfoliation of graphite in the presence of non-ionic/non-toxic macromolecule polyvinylpyrrolidone (PVP) [19], while single-wall CNT were made water-soluble by functionalization with DNA or anionic detergent sodium dodecylbenzenesulfonate (SDBS), as previously described [20,21]. Briefly, 500 mg of crystalline graphite powder (synthetic, 6 mm; Timcal, Bodio, Switzerland) were suspended in 100 ml of deionized water containing 1 g PVP (SigmaeAldrich, St. Louis, MO), while 50 mg of single-wall CNT (95%; BuckyUSA, Houston, TX) were added to 100 ml of deionized water containing 1 mg of heat-denatured single-stranded DNA or 2 mg SDBS (both from SigmaeAldrich, St. Louis, MO).…”

Changes of structure and electrical conductivity of multi-walled carbon nanotubes film caused by 3MeV proton irradiation

“…In Fig. 6(b)-(d) one can observe bands at about 2837 and 2920 cm −1 , which correspond to C H bonds in the nanotube matrix [29]. Moreover, intensity of the C H bonds increases with increasing the irradiation fluence.…”

Comparison of structural properties of pristine and gamma irradiated single-wall carbon nanotubes: Effects of medium and irradiation dose

“…All samples were irradiated with the dose of 50 kGy. In the curves 1, 2, 3 and 4 one can observe bands at around 2830 and 2900 cm −1 , which correspond to C\H bonds in the nanotube matrix [29]. The spectra of γ-air-SWCNTs (curve 2), γ-ammonia-SWCNTs (curve 3) and γ-water-SWCNTs (curve 4) exhibit broad band at 1550, which stem from C\O stretching vibration of COOH functional groups.…”

“…Authors confirmed that the changes in electrical conductivity and Young modulus correlate with the dose of gamma irradiation. Applying a relatively low dose of gamma irradiation on SWCNTs, it was observed that gamma irradiated nanotubes were more soluble in water compared to pristine ones [29].…”