A novel method for urinary tract infection diagnosis and antibiogram using Raman spectroscopy

Kastanos, Evdokia; Kyriakides, Alexandros; Hadjigeorgiou, Katerina; Pitris, Costas

doi:10.1002/jrs.2540

Cited by 34 publications

(35 citation statements)

References 39 publications

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“…One example is Raman spectro scopy, which has been used for the detection and identifi cation of bacteria in previous investigations 103,104 and could prove useful in establishing the make-up of the intestinal microbiota (for example, via measurements of tissue or faecal samples, or through in vivo endoscopic studies). Endocytoscopy, a form of white light endomicroscopy, can also visual ize normal villi 105 , but cannot detect the functional changes visualized by fluorescence endomicro scopy.…”

Section: Recommendationsmentioning

confidence: 99%

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

Thompson

Hughes

Anastasova

et al. 2017

Nat Rev Gastroenterol Hepatol

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| Environmental enteric dysfunction (EED) is a disease of the small intestine affecting children and adults in low and middle income countries. Arising as a consequence of repeated infections, gut inflammation results in impaired intestinal absorptive and barrier function, leading to poor nutrient uptake and ultimately to stunting and other developmental limitations. Progress towards new biomarkers and interventions for EED is hampered by the practical and ethical difficulties of cross-validation with the gold standard of biopsy and histology. Optical biopsy techniques -which can provide minimally invasive or noninvasive alternatives to biopsy -could offer other routes to validation and could potentially be used as point-of-care tests among the general population. This Consensus Statement identifies and reviews the most promising candidate optical biopsy technologies for applications in EED, critically assesses them against criteria identified for successful deployment in developing world settings, and proposes further lines of enquiry. Importantly, many of the techniques discussed could also be adapted to monitor the impaired intestinal barrier in other settings such as IBD, autoimmune enteropathies, coeliac disease, graft-versus-host disease, small intestinal transplantation or critical care. Members of the board and experts met to discuss the horizon scan in London, UK, in November 2015 and San Francisco, USA, in February 2016. Literature searches were performed using Web of Science, as well as through the advice of the advisory board and experts. The results of the horizon scan are presented in this Consensus Statement, which entails a review of EED, a discussion of technologies deemed relevant to EED (based on literature searches and the opinions of the advisory board and experts), and a series of recommendations for the future application of the rele vant technologies to the study and diagnosis of EED. In the Recommendations section, each technology has been assessed according to its suitability for application to EED and this assessment includes an evaluation of the cost, invasiveness and appropriateness for developing countries of each device and/or technique. This assessment was performed in a qualitative manner based on currently available information (for example, the present cost and size of the devices) and the opinions of the authors as to feasible future develop ment. Technique suitability for use in developing countries was determined in a qualitative manner after consideration of the need for advanced infrastructure and high levels of training for either system use or data analysis. Probable deployment time scales have also been assigned to each technique based on the current states of development and the degree of valid ation work that will be required before application to EED. These timescales have been tentatively defined as short-term (suitable for deployment immediately or within ~1 year), mediumterm (suit able for deployment within ~1-3 years) and long-term (suitable for deployme...

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Section: Recommendationsmentioning

confidence: 99%

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

Thompson

Hughes

Anastasova

et al. 2017

Nat Rev Gastroenterol Hepatol

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“…Recently, comprehensive reviews concerning the bacteria detection by using Raman and SERS spectroscopies were reported [15,48,49]. The increased sensitivity and high information content of SERS is acknowledged, mostly when this powerful tool is used in conjunction with advanced analysis and classification techniques.…”

Section: In Situ Ag Nps Synthesis: Extended Approachmentioning

confidence: 99%

The Intricate Nature of SERS: Real‐Life Applications and Challenges

Dina¹,

Colniță²

2017

Raman Spectroscopy and Applications

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Testing for the presence of microorganisms in biological samples in order to diagnose infections is very common at all levels of health care. There is a growing need to ensure appropriate diagnosis by also minimizing the analysis time, both being very important concerns related to the risk of developing an antimicrobial resistance. Moreover, there are important medical and financial implications associated with infections. In this chapter, we will discuss the latest ultrasensitive and selective, but simple, rapid and inexpensive bacteria detection and identification methods by using receptor-free and innovative immobilization principles of the biomass. Raman spectroscopy, which combines the selectivity of the method with the sensitivity of the surface-enhanced Raman scattering (SERS) effect, is used in correlation with chemometric techniques in order to develop biosensors for pathogenic microorganisms.

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“…Using this system, they were able to successfully discriminate between different pathogenic species using a diagnostic algorithm based on principal component analysis (PCA) of the acquired Raman spectra. Further investigation of the use of Raman spectroscopy in the study and diagnosis of UTIs has been performed by the research group of Costas Pitris [12][13][14][15]. In their research, the Pitris group used Raman spectroscopy to detect the presence of bacteria, to discriminate multiple bacterial species using PCA, and, finally, to assess the bacteria's response to antibiotic treatment [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Further investigation of the use of Raman spectroscopy in the study and diagnosis of UTIs has been performed by the research group of Costas Pitris [12][13][14][15]. In their research, the Pitris group used Raman spectroscopy to detect the presence of bacteria, to discriminate multiple bacterial species using PCA, and, finally, to assess the bacteria's response to antibiotic treatment [12][13][14][15]. Overall, this implies that Raman spectroscopy has the potential to become a useful clinical tool for early diagnosis of infection as it can permit both the detection and identification of relevant bacteria.…”

Section: Introductionmentioning

confidence: 99%

Towards optical fibre based Raman spectroscopy for the detection of surgical site infection

et al. 2016

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Surgical site infections (SSIs) are common post-surgical complications that remain significant clinical problems, as they are associated with substantial mortality and morbidity. As such, there is significant interest in the development of minimally invasive techniques that permit early detection of SSIs. To this end, we are applying a compact, clinically deployable Raman spectrometer coupled to an optical fibre probe to the study of bacteria, with the long term goal of using Raman spectroscopy to detect infection in vivo. Our system comprises a 785 nm laser diode for excitation and a commercial (Ocean Optics, Inc.) Raman spectrometer for detection. Here we discuss the design, optimisation and validation of this system, and describe our first experiences interrogating bacterial cells (Escherichia coli) in vitro.

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A novel method for urinary tract infection diagnosis and antibiogram using Raman spectroscopy

Cited by 34 publications

References 39 publications

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

The Intricate Nature of SERS: Real‐Life Applications and Challenges

Towards optical fibre based Raman spectroscopy for the detection of surgical site infection

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