A novel method of encapsulating and cultivating adherent mammalian cells within collagen microcarriers

Wu, Ta Jen; Huang, Hsiu-Hsuan; Hsu, Yuan-Ming; Lyu, Shaw-Ruey; Wang, Yng Jiin

doi:10.1002/bit.21452

Cited by 18 publications

(7 citation statements)

References 13 publications

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“…Previous studies have shown the proliferation and differentiation of myoblasts (cells lines and primary cells) in collagen matrix (Cheema, Yang et al, 2003;Rhim, Lowell et al, 2007;Wu, Huang et al, 2007). In those studies, cells were allowed to proliferate in GM prior to differentiation.…”

Section: C2c12 Single Cells and Aggregates Exhibit High Levels Of Promentioning

confidence: 99%

Aggregation promotes cell viability, proliferation, and differentiation in anin vitromodel of injection cell therapy

Bayoussef

Dixon

Stolnik

et al. 2011

J Tissue Eng Regen Med

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Many cell therapy approaches aim to deliver high-density single-cell suspensions to diseased or injured sites in the body. Long term clinical success will in part be dependent on the cells that remain viable and that assume correct functionality post-administration. The research presented in this paper focuses on the potential of cell aggregate delivery to generate a more supportive environment for cells than single cell suspensions. An in vitro model of injection delivery of C2C12 myoblast cells showed a significant difference in cell function and phenotype between adhesive collagen and non-adhesive alginate, indicating that in vitro assays based on this approach can discriminate between cell-cell/cell-matrix interactions and could be valuable when assessing cell therapy systems. Contrary to single cells, aggregates maintain viability, cellular activity, and phenotype beyond that of single cells, even in non-adhesive matrices, enabling delivery of higher cell densities with enhanced proliferative and differentiation capacity.

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Section: C2c12 Single Cells and Aggregates Exhibit High Levels Of Promentioning

confidence: 99%

Aggregation promotes cell viability, proliferation, and differentiation in anin vitromodel of injection cell therapy

Bayoussef

Dixon

Stolnik

et al. 2011

J Tissue Eng Regen Med

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“…Alginate, a natural polysaccharide isolated from brown seaweed, is the most common used gel for cell encapsulation due to its biocompatibility and stability in vivo (de Vos et al, 2002;Shoichet and Rein, 1996;Wu et al, 2007;Murua et al, 2008). Moreover, alginate has the capacity to protect encapsulated cells against recognition by the immune system (Barminko et al, 2011;Herrero et al, 2007;Kang et al, 2014;Trouche et al, 2010;Zanotti et al, 2013), which will enable the use of allogeneic cells.…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of allogeneic mesenchymal stem cells in alginate extends local presence and therapeutic function

Leijs¹,

Villafuertes²,

Haeck³

et al. 2017

eCM

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Bone marrow derived mesenchymal stem cells (MSCs) have immunomodulatory and trophic capacities. For therapeutic application in local chronic inflammatory diseases, MSCs, preferably of allogeneic origin, have to retain immunomodulatory properties. This might be achieved by encapsulation of MSCs in a biomaterial that protects them from the host immune system. Most studies investigating the properties of MSCs for therapeutic application use short term cultures of cells in monolayer.Since the physical environment of MSCs can influence their functionality, we evaluated the feasibility of preserving the immunomodulatory properties of MSCs encapsulated in a three-dimensional alginate construct.After 5 weeks of implantation in immunocompetent rats, active allogeneic MSCs encapsulated in alginate were still detectable by Bio Luminescence Imaging and Magnetic Resonance Imaging of luciferase transduced and superparamagnetic iron oxide labelled MSCs. MSCs injected in saline were only detectable up to 1 week after injection. Moreover, the MSCs encapsulated in alginate responded to inflammatory stimuli similarly to MSCs in monolayer culture. In addition, MSC-alginate beads secreted immunomodulatory and trophic factors and inhibited T-cell proliferation after 30 d of in vitro culture. Our data indicate that allogeneic MSCs encapsulated in alginate persist locally and could act as an interactive immunomodulatory or trophic factor release system for several weeks, making this an interesting system to investigate for application in inflammatory disease conditions.

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“…In addition to alginates and combinations with chitosan (108) or agarose (109), biomaterials like collagen (110), hyaluronic acid (111) and dextran (112) have also been used. For these applications, the materials should have the ability to form a gel in physiological conditions (temperature, pH and ionic strength).…”

Section: Cell Encapsulation For Drug Deliverymentioning

confidence: 99%

Controlled Delivery Systems: From Pharmaceuticals to Cells and Genes

2011

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During the last few decades, a fair amount of scientific investigation has focused on developing novel and efficient drug delivery systems. According to different clinical needs, specific biopharmaceutical carriers have been proposed. Micro- and nanoparticulated systems, membranes and films, gels and even microelectronic chips have been successfully applied in order to deliver biopharmaceuticals via different anatomical routes. The ultimate goal is to deliver the potential drugs to target tissues, where regeneration or therapies (chemotherapy, antibiotics, and analgesics) are needed. Thereby, the bioactive molecule should be protected against environmental degradation. Delivery should be achieved in a dose- and time-correct manner. Drug delivery systems (DDS) have been conceived to provide improvements in drug administration such as ability to enhance the stability, absorption and therapeutic concentration of the molecules in combination with a long-term and controlled release of the drug. Moreover, the adverse effects related with some drugs can be reduced, and patient compliance could be improved. Recent advances in biotechnology, pharmaceutical sciences, molecular biology, polymer chemistry and nanotechnology are now opening up exciting possibilities in the field of DDS. However, it is also recognized that there are several key obstacles to overcome in bringing such approaches into routine clinical use. This review describes the present state-of-the-art DDS, with examples of current clinical applications, and the promises and challenges for the future in this innovative field.

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A novel method of encapsulating and cultivating adherent mammalian cells within collagen microcarriers

Cited by 18 publications

References 13 publications

Aggregation promotes cell viability, proliferation, and differentiation in anin vitromodel of injection cell therapy

Aggregation promotes cell viability, proliferation, and differentiation in anin vitromodel of injection cell therapy

Encapsulation of allogeneic mesenchymal stem cells in alginate extends local presence and therapeutic function

Controlled Delivery Systems: From Pharmaceuticals to Cells and Genes

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