A Novel Method to Determine the Resistance of Biotemplated Nanowires

Pussepitiyalage

et al. 2020

Biotechnology Journal

Biomolecules are increasingly attractive templates for the synthesis of functional nanomaterials. Chief among them is the plant tobacco mosaic virus (TMV) due to its high aspect ratio, narrow size distribution, diverse biochemical functionalities presented on the surface, and compatibility with a number of chemical conjugations. These properties are also easily manipulated by genetic modification to enable the synthesis of a range of metallic and non-metallic nanomaterials for diverse applications. This article reviews the characteristics of TMV and related viruses, and their virus-like particle (VLP) derivatives, and how these may be manipulated to extend their use and function. A focus of recent efforts has been on greater understanding and control of the self-assembly processes that drive biotemplate formation. How these features have been exploited in engineering applications such as, sensing, catalysis, and energy storage are briefly outlined. While control of VLP surface features is well-established, fewer tools exist to control VLP self-assembly, which limits efforts to control template uniformity and synthesis of certain templated nanomaterials. However, emerging advances in synthetic biology, machine learning, and other fields promise to accelerate efforts to control template uniformity and nanomaterial synthesis enabling more widescale industrial use of VLP-based biotemplates.

Section: Nanostructure Synthesis With Viral Biotemplates For Diverse mentioning

confidence: 99%

Engineering Tobacco Mosaic Virus and Its Virus‐Like‐Particles for Synthesis of Biotemplated Nanomaterials

Pussepitiyalage

et al. 2020

Biotechnology Journal

Section: Nanostructure Synthesis With Viral Biotemplates For Diverse Applicationsmentioning

confidence: 99%

Engineering Tobacco Mosaic Virus, Barley Stripe Mosaic Virus, and their Virus-Like-Particles for Synthesis of Biotemplated Nanomaterials

Pussepitiya

et al. 2020

Preprint

Self Cite

Biomolecules are increasingly attractive templates for the synthesis of functional nanomaterials. Chief among them are the plant Tobacco mosaic virus (TMV) and Barley stripe mosaic virus (BSMV) due to their high aspect ratio, narrow size distribution, diverse biochemical functionalities presented on the surface, and compatibility with a number of chemical conjugations. These properties are also easily manipulated by genetic modification to enable the synthesis of a range of metallic and non-metallic nanomaterials for diverse applications. This article reviews the characteristics of TMV, BSMV, and their virus-like particle (VLP) derivatives and how these may be manipulated to extend their use and function. A focus of recent efforts has been on greater understanding and control of the self-assembly processes that drive biotemplate formation. We briefly outline how these features have been exploited in engineering applications such as sensing, catalysis, and energy storage, and discuss emerging advances that promise to accelerate the development of these biotemplates for widescale industrial use.

“…Directed synthesis of nanomaterials with controllable dimensions remains a subject of immediate concern in nanotechnology. , Biotemplating, the use of naturally occurring biomolecules to develop nanomaterials of similar morphology, hierarchical complexity and nanometric precision, is a viable means to easily and inexpensively produce needed nanomaterials on a large scale . Specifically, the use of viruses as biotemplates has been pursued as a potent solution due to their unique advantages for applications in catalysis, − nanocircuitry, − chemical sensing, − biocatalysis, memory devices, and light harvesting. , As many studies of the M13 bacteriophage and the Tobacco mosaic virus (TMV) have shown, virus templates are particularly exciting due to the wide range of chemical interactions afforded by their multifunctional protein surfaces. The potential presence of polar, hydrophilic, charged, acidic, and basic functionalities on a single template allow the formation of a wide range of inorganic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

BSMV as a Biotemplate for Palladium Nanomaterial Synthesis

et al. 2017

The vast unexplored virus biodiversity makes the application of virus templates to nanomaterial synthesis especially promising. Here, a new biotemplate, Barley stripe mosaic virus (BSMV) was successfully used to synthesize organic-metal nanorods of similarly high quality to those produced with Tobacco mosaic virus (TMV). The mineralization behavior was characterized in terms of the reduction and adsorption of precursor and nanocrystal formation processes. The BSMV surface-mediated reduction of Pd proceeded via first-order kinetics in both Pd and BSMV. The adsorption equilibrium relationship of PdClHO on the BSMV surface was described by a multistep Langmuir isotherm suggesting alternative adsorbate-adsorbent interactions when compared to those on TMV. It was deduced that the first local isotherm is governed by electrostatically driven adsorption, which is then followed by sorption driven by covalent affinity of metal precursor molecules for amino acid residues. Furthermore, the total adsorption capacity of palladium species on BSMV is more than double of that on TMV. Finally, study of the BSMV-Pd particles by combining USAXS and SAXS enabled the characterization of all length scales in the synthesized nanomaterials. Results confirm the presence of core-shell cylindrical particles with 1-2 nm grains. The nanorods were uniform and monodisperse, with controllable diameters and therefore, of similar quality to those synthesized with TMV. Overall, BSMV has been confirmed as a viable alternate biotemplate with unique biomineralization behavior. With these results, the biotemplate toolbox has been expanded for the synthesis of new materials and comparative study of biomineralization processes.