A Novel Method to Overcome Coat-Imposed Seed Dormancy in Lupinus albus L. and Trifolium pratense L.

Çil²,

International Journal of Agronomy

2016

Self Cite

The seed coat colour variation of 70 common vetch genotypes were determined by using uniform colour scale(L⁎a⁎b⁎)and their possible correlation with seed yield parameters including the number of pods per plant, the number of seeds per pod, pod dimension, and seed yield (kg/da) was determined. The results revealed presence of highly significant (p<0.01) variations for both the seed yield and the seed coat colour parameters measured. The number of pods per plant, the number of seeds per pod, and seed yield ranged from 5.8 to 16.03, from 5.2 to 7.66, and from 143.37 to 531.1, respectively. The lightness value varied from 19.00 to 40.28 while chromaticitya⁎andb⁎values ranged from −0.16 to 8.99 and from 0.79 to 22.11, respectively. The highest correlation coefficients were determined betweenb⁎andL⁎(r=0.73), anda⁎andL⁎(r=0.55). The seed coat colour traits and seed yield parameters generally showed weak negative correlations. Seed yellowness (b⁎) and seed yield had correlation coefficient of −0.25, while correlation betweenL⁎and seed yield was determined as −0.23. The results indicated that lightness and yellowness of seed coat may be used as an important parameter to prescreen high yield genotypes of common vetch.

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Revealing Seed Coat Colour Variation and Their Possible Association with Seed Yield Parameters in Common Vetch (Vicia sativaL.)

Çil²,

International Journal of Agronomy

2016

Self Cite

“…This finding is in agreement with reports from literature. Regulated heat has been reported to not only break dormancy and speed up germination rate, but also control certain seed-borne diseases of plants (Schmitt et al, 2009;Tiryaki and Topu, 2014;Nandini and Kulkarni, 2015).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of heat treatment and bio-priming of cowpea seeds for the management of fusarium wilt disease in the screen house

Ajayi¹,

Evbenata²

2020

J. Agric. Crop Res

Fusarium wilt is one of the most devastating diseases of cowpea. The pathogen, Fusarium oxysporum f.sp. tracheiphilum is soil and seed-borne. A non-chemical approach was designed for the management of the disease in the present study. Hot water (physical) at three temperatures, 40 50 and 60°C, and Trichoderma viride (biological) at 10 6 spores/ml were evaluated singly and synergistically in the laboratory. The duration of exposure to hot water was 5 min, after which seeds were allowed to cool followed by priming in sterile water (sole hot water treatment) or T. viride (synergistic physical and bio-control) medium in Petri-dishes for 72 h. The control consisted of seed treated with water at ambient temperature, while the standard check was seeds dressed with Mancozeb fungicide. Both were primed in a sterile water medium. Nine treatments were evaluated in all. Germinated seeds were transplanted into plastic pots containing soil infested with F. oxysporum at 10 6 spores/ml in the screen house. The experimental layout was completely randomized design and data collected were subjected to statistical analysis and mean separation. Results showed that seeds treated with 50°C hot water only had the highest germination percentage, 96.66%, and seedling vigour, 460.98. Disease incidence and severity values were least in seeds treated with synergistic hot water at 50°C and priming in T. viride medium. The highest number (77.33) and weight (13.41 g) of seeds were also recorded for the same treatment, while the least, 18 and 3.60 g were obtained from control. Integrated use of hot water (50°C) and bio-priming in T. viride medium can be recommended for use in the management of fusarium wilt disease of cowpea.

“…Cunningham, in which a GP of 55 % was achieved for seeds exposed to water at 80 °C for 2 min (Sánchez et al 2005). According to Tiryaki & Topu (2014), thermal treatments such as abrupt changes in temperature cause the seed testa to crack because the cells expand in the hot water and then contract when exposed to ambient temperature. The treatment with sulfuric acid was also able to break the seed dormancy of the species studied, although the germination percentages were lower than those reported for other Lupinus species, and in our study were GP values were even different for the same species from different regions of Mexico.…”

Section: Discussionmentioning

confidence: 99%

Comparison of pre-germination treatments in Lupinus spp. and their effects on germination and related solutes

et al. 2017

Background: Physical dormancy in seeds of the genus Lupinus restricts their ecological or agricultural use. Hypothesis: This dormancy can be broken when seeds are subjected to physical and chemical pretreatments that increase germination, mobilize reserves and decrease ABA. Studied species: Seeds of Lupinus exaltatus (Le), L. campestris (Lc) and L. montanus (Lm) from Puebla, México. Methods:The following seven treatments, including a control, were applied: PG1 = 98 % H 2 SO 4 for 15 min, PG2 = wet sand at 80 °C for 5 min, PG3 = wet sand at 35 °C for 8 h and 16 h at 25 °C, PG4 = dry sand at 80 °C for 7 min, PG5 = dry sand at 150 °C for 1 min, PG6 = H 2 0 at 80 °C for 1 min, Control = untreated seeds. On days 0, 3, 5, 10 and 15 after seeding, we evaluated the percentage and rate of germination (GP and GR, respectively) and biochemical changes. Results: PG6 produced a higher GP in Le (41 %) and Lc (69 %), and PG1 produced a higher GP in Lm (37 %). In all three species, the highest GR was obtained with PG1 (1.95, 2.27 and 2.22 day -1 seeds, respectively). PG6 increased the protein concentration (53, 17, and 43 % for Le, Lc and Lm, respectively), amino acids (19, 44 and 31 %, respectively), reducing sugars (63, 18 and 96 %, respectively) and polyphenols (32, 55 and 75 %, respectively) but decreased soluble sugars (22, 29 and 23 %, respectively) and ABA relative to the control. Although only the correlation between the GP and amino acid content was significant and positive, and the correlation between GP, GR and soluble sugars was significant and negative. Conclusions: The effect of pre-germination treatments on germination was species-specific. The pretreatment with H 2 0 at 80 °C for 1 min increased germination and mobilized the seed's reserves in the process. Key words: Lupinus, physical dormancy, germination, protein, sugars. ResumenAntecedentes: La latencia física en semillas del género Lupinus restringe su uso ecológico o agronómico. Hipótesis: Esta latencia puede liberarse al aplicarles pre-tratamientos físicos y químicos, lo que incrementa la germinación, moviliza reservas y disminuye el ABA. Resultados: El PG6 promovió mayor PG en Le (41 %) y Lc (69 %), y el PG1 en Lm (37 %). En las tres especies, la mayor VG fue con el PG1 (1.95, 2.27 y 2.22 semillas día -1 , respectivamente). Con PG6, se observó un incremento en concentración de proteína (53, 17, 43 % para Le, Lc y Lm, respectivamente), aminoácidos (19, 44 y 31 %), azú-cares reductores (63, 18 y 96 %) y polifenoles (32, 55 y 75 %) pero disminuyeron los azúcares solubles (22, 29 y 23 %) y ABA respecto al control. Aunque solo la correlación entre PG y aminoácidos fue significativa y positiva y entre PG, VG y azúcares solubles es significativa y negativa. Conclusiones: Entre especies, el efecto de los tratamientos pre-germinativos sobre la germinación fue diferencial. El uso de H 2 0 80 °C 1 min incrementó germinación y movilizó las reservas durante el proceso.