A novel microbial BOD biosensor developed by the immobilization of P. Syringae in micro‐cellular polymers

Abstract

BACKGROUND: A biochemical oxygen demand (BOD) sensor, based on an immobilized Pseudomonas syringae in highly porous micro-cellular polymer (MCP) in combination with a dissolved oxygen electrode, has been developed for the analysis of biodegradable organic compounds in aqueous samples. Microorganisms were immobilized in a molded MCP disk and a wastewater sample was injected into the biocomposite disk by a flow injection system. Dissolved oxygen (DO) changes as a measure of soluble BOD was read with a DO probe placed into a flow cell carrying biocatalytically activated disk.

An innovative reactor-type biosensor for BOD rapid measurement

“…It can be seen that the temperature effect was consistent with the results reported by other researchers (Chee et al, 1999a(Chee et al, , 1999b 2000; Liu et al, 2000;Kim and Park, 2001;Kwok et al, 2005;Seo et al, 2009;Kara et al, 2009;Tan and Qian, 1997). Simultaneously, we could observe from Fig.…”

“…Karube et al (1977) reported the first BOD sensor based on the principle of biosensor. Since then, BOD biosensor has been developed in last three decades (Liu et al, 2004a,b;Chen et al, 2008a,b;Kara et al, 2009). However, all these BOD biosensors developed were membrane-type, i.e., they tended to immobilize the microbial cells in a porous membrane such as nitrate cellulose, acetate cellulose membrane and the like.…”

Microbial Biosensors for Environmental Monitoring and Food Analysis

“…Moreover, a BOD microbial biosensor, where the biocatalyst (microbial/enzymatic) is placed between cellulose and Teon membranes installed on a dissolved oxygen (DO) probe, has been newly developed, which could offer better stability and lifetime for commercial use in environmental monitoring. (39) …”

Sensors for measuring biodegradable and total organic matter in water

“…Microorganisms are entrapped on columns [35] or immobilized on membranes [36][37][38][39][40][41][42][43]. The microorganisms used are pure strains with or without enzymes [36][37][38][39], consortia of mixed strains , or inocula from wastewater-treatment plant (WWTPs) [39,40], synthetic wastewater [41], brackish waters [43] or standardized microbial cultures [42]. Inocula metabolize a broader spectrum of substrates but present a less stable composition and are consequently less constant over time.…”

“…BOM can also be monitored in anaerobic processes [44]. Various papers have reported on biofilm optimization [45], immobilization mode [37,38] and inoculum renewal [41]. Electrolyte consumption and anodic oxidation limit the Clark electrode lifetime, and require the anode surface to be ground of and regular electrolyte renewal to avoid signal drift.…”