A novel microfluidic compact disc to investigate electrochemical property changes between artificial and real salivary samples mixed with mouthwashes using electrical impedance analysis

Abstract

Diagnosing oral diseases at an early stage may lead to better preventive treatments, thus reducing treatment burden and costs. This paper introduces a systematic design of a microfluidic compact disc (CD) consisting of six unique chambers that run simultaneously from sample loading, holding, mixing and analysis. In this study, the electrochemical property changes between real saliva and artificial saliva mixed with three different types of mouthwashes (i.e. chlorhexidine-, fluoride- and essential oil (Listerine)-based mouthwashes) were investigated using electrical impedance analysis. Given the diversity and complexity of patient’s salivary samples, we investigated the electrochemical impedance property of healthy real saliva mixed with different types of mouthwashes to understand the different electrochemical property which could be a foundation for diagnosis and monitoring of oral diseases. On the other hand, electrochemical impedance property of artificial saliva, a commonly used moisturizing agent and lubricant for the treatment of xerostomia or dry mouth syndrome was also studied. The findings indicate that artificial saliva and fluoride-based mouthwash showed higher conductance values compared to real saliva and two other different types of mouthwashes. The ability of our new microfluidic CD platform to perform multiplex processes and detection of electrochemical property of different types of saliva and mouthwashes is a fundamental concept for future research on salivary theranostics using point-of-care microfluidic CD platform.

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“…When the liquid was loaded, the disc was then placed on the spin system and spun with a gradual increase of RPM (revolutions per minute) ranging from 0 to 1000 RPM. A microcontroller (ATmega328) was used for controlling the stepper motor rotation, consistent with our previous reports [21][22][23]. Testo 477 LED stroboscope lighting was synced with the RPM, to provide a still image-like video for observation of the liquid's movement in the disc, as shown in Figure 3.…”

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“…Furthermore, Thita et al introduced a systematic and automated design of a microfluidic compact disc (CD) to investigate the electrochemical property changes of saliva after mixing with various types of mouthwashes using electrical impedance analysis. The developed model has demonstrated the potential of salivary theragnostic research [ 211 ].…”