A novel monoclonal antibody to fibrin monomer and soluble fibrin for the detection of soluble fibrin in plasma

Plasma Soluble Fibrin Monomer Complex as a Marker of Coronary Thrombotic Events in Patients with Acute Myocardial Infarction

“…Recently, three novel immunoassays for SF and FMC were developed with specific monoclonal antibodies (Soe et al 1996;Hamano et al 2002;Suzuki et al 2007). The monoclonal antibody IF-43 is directed against the neo-epitope present on the α -chain remnant corresponding to the A α residues (52-78) in the E domain of the desAA fibrin monomer, which is prepared from a urea-solubilized human fibrin monomer.…”

“…The complexes detected with the monoclonal antibodies IF-43 and J2-23 are generally termed SF, while those detected with F405 are termed FMC. The monoclonal antibody F405 is directed against a desAA fibrin monomer-specific neo-epitope ] generated by thrombin-induced cleavage of FPA from fibrinogen (Hamano et al 2002). F405 is also able to recognize the desAA and desAABB fibrin monomers, as well as the X-, Y-, and E-fragments of fibrin monomer, though not fibrinogen, fragments of fibrinogen, or XDP including D-dimer (DD) (Hamano et al 2002).…”

“…The monoclonal antibody F405 is directed against a desAA fibrin monomer-specific neo-epitope ] generated by thrombin-induced cleavage of FPA from fibrinogen (Hamano et al 2002). F405 is also able to recognize the desAA and desAABB fibrin monomers, as well as the X-, Y-, and E-fragments of fibrin monomer, though not fibrinogen, fragments of fibrinogen, or XDP including D-dimer (DD) (Hamano et al 2002). FMC is considered to be a useful marker for hypercoagulable states with accelerated fibrinolysis, because F405 also recognizes the degradation products of fibrin monomer by fibrinolytic activity and their complexes with fibrin(ogen) (Ieko et al 2007).…”

High Macrophage Migration Inhibitory Factor Levels in Disseminated Intravascular Coagulation Patients with Systemic Inflammation

“…Especially, we found a close correlation between the levels of soluble fibrin and MIF. Soluble fibrin composed of fibrin monomer and fibrinogen is well known to be a sensitive marker of thrombin activation [21]. This significant correlation indicates a link between thrombin and MIF.…”

ISTH overt disseminated intravascular coagulation score in patients with septic shock: automated immunoturbidimetric soluble fibrin assay vs. D‐dimer assay

“…DDi plasma concentrations were assayed using an immuno-turbidimetric method (STA Ò -Liatest Ò D-Di), as were soluble fibrin monomer complexes concentrations (STA Ò -Liatest Ò FM). This assay uses the F405 [7] monoclonal antibody coated on latex microparticles: the recognized epitope was the alpha-chain N-terminal region exposed upon removal of the A peptide from the Aalpha-chain, F405 binding to the alpha-chain N-terminal oligo-peptide of fibrin (GPRVVERHQ) [7].…”