A novel nondrug SFRP1 antagonist inhibits catagen development in human hair follicles
            <i>ex vivo</i>

Bertolini, Marta; Chéret, Jérémy; Pinto, Daniela; Hawkshaw, Nathan J; Ponce, L.; Erdmann, Hanieh; Jiménez, Francisco; Funk, Wolfgang; Paus, Ralf

doi:10.1111/bjd.19552

Cited by 8 publications

(8 citation statements)

References 8 publications

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“…The point photograph presented some overlapping genes in multiple compared groups especially back skin compared with other skin sites, including TNN, COL11A1, SFRP1, COL6A5, INHBA, APOA1, IGF1 and SPARCL1. Notably, TNN, called tenascin-N(W), is lager domain glycoprotein that has the potential to modify cell adhesion and typically contribute to cell motility (Chiquet-Ehrismann and Tucker, 2011); COL11A1, an extracellular matrix structural constituent, comprises a subclass of regulatory collagens fibrillogenesis that synergistically assemble other types of collagen such as collagen I, determining fibril structure, fibril organization and functional traits (Smith and Birk, 2012; Sun et al, 2020); SFRP1, a member of secretory glycoprotein SFRP family, is regarded as one of the main classes of macromolecules making up the ECM elements and is reported to be an antagonist that inhibits human hair follicles recession (Bertolini et al, 2021; Jiang et al, 2022); INHBA is a member of TGFβ superfamily and is modified by AP1 expression (Ham et al, 2021). Subsequently, we implemented KEGG analysis in the compared groups (Figure 5—figure supplement 1F, G) and presented dominant enrichment pathways such as ECM-receptor interaction, focal adhesion, protein digestion and adsorption and TGF-beta signaling pathway.…”

Section: Resultsmentioning

confidence: 99%

“…The copyright holder for this preprint this version posted February 18, 2023. ; https://doi.org/10.1101/2023.02.17.528908 doi: bioRxiv preprint (Bertolini et al, 2021;Jiang et al, 2022); INHBA is a member of TGFβ superfamily and is modified by AP1 expression (Ham et al, 2021). Subsequently, we implemented KEGG analysis in the compared groups (Figure 5-figure supplement 1F, G) and presented dominant enrichment pathways such as ECM-receptor interaction, focal adhesion, protein digestion and adsorption and TGF-beta signaling pathway.…”

Section: Heterogeneity Of the Fibroblastmentioning

confidence: 99%

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Single-cell transcriptome analysis on the anatomic positional heterogeneity of pig skin

Zou

Yuan

Wang

et al. 2023

Preprint

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Different anatomic locations of the body skin dermis come from different origins, and its positional hereditary information can be maintained in adults, while highly resolvable cellular specialization is less well characterized in different anatomical regions. Pig is regarded as excellent model for human research in view of its similar physiology to human. In this study, we performed single-cell RNA sequencing of six different anatomical skin regions from the Chenghua pig with superior skin thickness traits. We obtained 215,274 cells, representing seven cell types, among which we primarily characterized the heterogeneity of smooth muscle cells, endothelial cells and fibroblasts. We identified several phenotypes of smooth muscle cell and endothelial cell and presented genes expression of pathways such as the immune response in different skin regions. By comparing differentially expressed fibroblast genes among different skin regions, we considered TNN, COL11A1, and INHBA as candidate genes for facilitating ECM accumulation. These findings of heterogeneity in the main three cell types from different anatomic skin sites will contribute to a better understanding of hereditary information and places the potential focus on skin generation, transmission and transplantation, paving the foundation for human skin priming.

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Section: Resultsmentioning

confidence: 99%

Section: Heterogeneity Of the Fibroblastmentioning

confidence: 99%

Single-cell transcriptome analysis on the anatomic positional heterogeneity of pig skin

Zou

Yuan

Wang

et al. 2023

Preprint

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“…For IGF‐1 [32] and FGF‐7 [55] expression, cryosections were fixed in acetone at −20°C and pre‐incubated with 2% normal goat serum in TNB buffer (Tris‐HCl + NaCl + Casein) or 10% NGS for 30 min at room temperature (RT) prior to overnight incubation at 4°C with a rabbit anti‐human IGF‐1 primary antibody (1/200 in 2% NGS, Abcam) or a rabbit anti‐human FGF‐7 primary antibody (1/150 in 10% NGS, Abcam). Secondary antibody incubation was performed at RT for 45 min using a goat anti‐rabbit Alexa Fluor 488 (1/400 in 2% NGS (IGF‐1) or 1/750 in 10% NGS (FGF‐7), Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

“…The latter is sustained by the expression of the extracellular matrix protein, versican and the expression and activity of alkaline phosphatase in the hair follicle dermal papilla [27][28][29][30]. Notably, dermal papilla inductivity is linked to the production of Wnt molecules that stimulate the proliferation of hair matrix keratinocytes [27,31,32]. Importantly, DP inductivity is decreased in androgenetic alopecia and telogen effluvium [33].…”

Section: Introductionmentioning

confidence: 99%

An extract of Leontopodium alpinum inhibits catagen development ex vivo and increases hair density in vivo

Campiche¹,

Riche

Edelkamp

et al. 2022

Intern J of Cosmetic Sci

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Objectives: Hair loss and reduction in hair volume are hallmarks of hair disorders, such as telogen effluvium, or male or female pattern hair loss, and hair ageing, which can cause severe distress in both men and women. Common antihair loss drugs carry some side effects; therefore, novel, safer approaches targeting milder phenotypes are highly advocated. In this context, we investigated an extract of the alpine plant Edelweiss, Leontopodium alpinum var. Helvetia, for its ability to modulate hair follicle (HF) growth ex vivo and inhibit hair loss while increasing hair regeneration in vivo.Methods: Human amputated HFs were microdissected from three donors, two women and one man, and cultured ex vivo for 6 days. After treatment with 0.001% Edelweiss extract (EWDE), we investigated hair shaft production and anagen/catagen conversion, and measured known parameters associated with hair growth, that is hair matrix keratinocyte proliferation and apoptosis, dermal papilla inductivity, and growth factors, by quantitative (immuno)histomorphometry. To assess the anti-hair loss potential of the alpine plant compound, we performed a randomized, placebo-controlled human study enrolling Caucasian women and men, aged 18 to 65 years, with normal hair loss. After 5 months' daily use of an extract containing leave-on serum, we analysed hair density and anagen-to-catagen/telogen ratio by the Trichogram analysis. Results:Our results revealed a significant prolongation in the anagen phase in HFs treated with 0.001% Edelweiss, as indicated by an increase in HFs remaining in anagen and a significant decrease in hair cycle score. In line with this effect, EWDE significantly stimulated hair matrix (HM) keratinocyte proliferation, and dermal papilla inductivity, as shown by a significant up-regulation of versican expression and alkaline phosphatase activity, and a tendential increase in FGF7 immunoreactivity in the dermal papilla of all HFs or only anagen VI HFs.Corroborating the ex vivo results, we observed a significant increase in growingThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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“…This has identified a novel regulatory principle in human hair growth control and has encouraged the development of non-immunosuppressive SFRP1-inhibitory agents as a novel class of human hair growth promoters. 67,179 Instead, given the recently appreciated pathogenic role of excessive epidermal Wnt activity in psoriatic lesions, 180,181 topically applicable drugs that up-regulate the production of endogenous Wnt inhibitors in human skin, such as SFRP1 and DKK1, 68…”

Section: Tr Ans L Ational Rele Van Ce and Con Cluding Remark Smentioning

confidence: 99%

The hair follicle‐psoriasis axis: Shared regulatory mechanisms and therapeutic targets

Suzuki

Ito

Gilhar

et al. 2021

Experimental Dermatology

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Physicians and investigators interested in either psoriasis or hair growth rarely interact to discuss pathways and mechanisms of potential common interest. And yet, it has long been recognized that both fields intersect closely and show more overlap than is widely appreciated. [1][2][3][4][5][6][7][8][9] Here, we review the relevant literature that points to a special relationship between the hair follicle (HF) and psoriasis and explain why a systematic re-examination of this axis is clinically relevant and promises to enrich both psoriasis and hair research.After we had first postulated that the induction of a psoriatic epidermal lesion and of anagen hair growth share selected "switch-on" mechanisms, 2 this concept was re-voiced in part two decades later 7 yet remains largely ignored and has not been rigorously probed until

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A novel nondrug SFRP1 antagonist inhibits catagen development in human hair follicles ex vivo

Abstract: Prevalence, correlates and clinical usefulness of antibodies to RNA polymerase III in systemic sclerosis: a cross-sectional analysis of data from an Australian cohort.

Cited by 8 publications

References 8 publications

Single-cell transcriptome analysis on the anatomic positional heterogeneity of pig skin

Single-cell transcriptome analysis on the anatomic positional heterogeneity of pig skin

An extract of Leontopodium alpinum inhibits catagen development ex vivo and increases hair density in vivo

The hair follicle‐psoriasis axis: Shared regulatory mechanisms and therapeutic targets

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