A Novel Nonthermal Energy Source for Surgical Epicardial Atrial Ablation: Irreversible Electroporation

Lavee, Jacob; Onik, Gary; Mikus, Paul; Rubinsky, Boris

doi:10.1532/hsf98.20061202

Cited by 148 publications

(96 citation statements)

References 28 publications

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“…Again, we calculated the change in the temperature ΔT of the medium using (7). In this case, the maximum increase in the temperature was 0.7 K and was generated by a train of 128 pulses.…”

Section: B Relation Between the Pulse Amplitude And The Number Of Pumentioning

confidence: 99%

“…E LECTROPORATION, as a method for increasing cell membrane permeability to molecules that are otherwise poorly membrane permeant, is used in various biotechnological and biomedical applications, such as the introduction of molecules into cells [1], [2], cell fusion [3], [4], tissue ablation [5]- [7], and sterilization of water and liquid food [8]- [10]. In experimental settings, electroporation is normally performed by placing a biological sample (e.g., cell suspension or a small part of a tissue) between the electrodes and delivering a single electric pulse or a train of such pulses to the electrodes, thus creating the electric field between them.…”

Section: Introductionmentioning

confidence: 99%

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Equivalent Pulse Parameters for Electroporation

Pucihar

Krmelj

Reberšek

et al. 2011

IEEE Trans. Biomed. Eng.

191

109

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Abstract-Electroporation-based applications require the use of specific pulse parameters for a successful outcome. When recommended values of pulse parameters cannot be set, similar outcomes can be obtained by using equivalent pulse parameters. We determined the relations between the amplitude and duration/number of pulses resulting in the same fraction of electroporated cells. Pulse duration was varied from 150 ns to 100 ms, and the number of pulses from 1 to 128. Fura 2-AM was used to determine electroporation of cells to Ca 2+ . With longer pulses or higher number of pulses, lower amplitudes are needed for the same fraction of electroporated cells. The expression derived from the model of electroporation could describe the measured data on the whole interval of pulse durations. In a narrower range (0.1-100 ms), less complex, logarithmic or power functions could be used instead. The relation between amplitude and number of pulses could best be described with a power function or an exponential function. We show that relatively simple two-parameter power or logarithmic functions are useful when equivalent pulse parameters for electroporation are sought. Such mathematical relations between pulse parameters can be important in planning of electroporationbased treatments, such as electrochemotherapy and nonthermal irreversible electroporation.

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“…Again, we calculated the change in the temperature ΔT of the medium using (7). In this case, the maximum increase in the temperature was 0.7 K and was generated by a train of 128 pulses.…”

Section: B Relation Between the Pulse Amplitude And The Number Of Pumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Equivalent Pulse Parameters for Electroporation

Pucihar

Krmelj

Reberšek

et al. 2011

IEEE Trans. Biomed. Eng.

191

109

View full text Add to dashboard Cite

show abstract

“…Further, it has been tested in animal models for treatment of cardiac disease by our group and others 10, 11, 12, 13, 14, 15, 16, 17, 18. Preliminary studies suggest that IRE may be a promising alternative ablation method for AF management.…”

Section: Introductionmentioning

confidence: 99%

Intrapulmonary Vein Ablation Without Stenosis: A Novel Balloon‐Based Direct Current Electroporation Approach

Witt

Sugrue

Padmanabhan

et al. 2018

JAHA

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BackgroundCurrent thermal ablation methods for atrial fibrillation, including radiofrequency and cryoablation, have a suboptimal success rate. To avoid pulmonary vein (PV) stenosis, ablation is performed outside of the PV, despite the importance of triggers inside the vein. We previously reported on the acute effects of a novel direct current electroporation approach with a balloon catheter to create lesions inside the PVs in addition to the antrum. In this study, we aimed to determine whether the effects created by this nonthermal ablation method were associated with irreversible lesions and whether PV stenosis or other adverse effects occurred after a survival period.Methods and ResultsInitial and survival studies were performed in 5 canines. At the initial study, the balloon catheter was inflated to contact the antrum and interior of the PV. Direct current energy was delivered between 2 electrodes on the catheter in ECG‐gated 100 μs pulses. A total of 10 PVs were treated demonstrating significant acute local electrogram diminution (mean amplitude decrease of 61.2±19.8%). After the survival period (mean 27 days), computed tomography imaging showed no PV stenosis. On histologic evaluation, transmural, although not circumferential, lesions were seen in each treated vein. No PV stenosis or esophageal injury was present.ConclusionsIrreversible, transmural lesions can be created inside the PV without evidence of stenosis after a 27‐day survival period using this balloon‐based direct current ablation approach. These early data show promise for an ablation approach that could directly treat PV triggers in addition to traditional PV antrum ablation.

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“…It occurs most commonly in the "vulnerable" period of the cardiac cycle during atrial or ventricular systole. 18,27,28 In addition, research subjects or patients with previously documented underlying structural or functional heart disease appeared to be at a greater risk for cardiac arrhythmia. No mortality has been associated with IRE-induced cardiac arrhythmia.…”

Section: More Questions Regarding Irementioning

confidence: 99%

Irreversible Electroporation: A Novel Image-Guided Cancer Therapy

2010

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Irreversible electroporation (IRE) is a novel tumor ablation technique using a non-thermal energy to create innumerable permanent nanopores in the cell membrane to disrupt cellular homeostasis. This disruption of cellular homeostasis initiates apoptosis which leads to permanent cell death. In our translational research, we have demonstrated that IRE can be a safe, fast and powerful method of tumor treatment. In this review, we present an overview of IRE ablation including a brief history of IRE, advantages and disadvantages of IRE and clinical and research implications of IRE. (Gut Liver 2010;4(Suppl. 1): S99-104)

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A Novel Nonthermal Energy Source for Surgical Epicardial Atrial Ablation: Irreversible Electroporation

Abstract: We propose a new modality to perform atrial ablations, which holds the potential of providing very swift, precise, and complete transmurality with no local heating effects.

Cited by 148 publications

References 28 publications

Equivalent Pulse Parameters for Electroporation

Equivalent Pulse Parameters for Electroporation

Intrapulmonary Vein Ablation Without Stenosis: A Novel Balloon‐Based Direct Current Electroporation Approach

Irreversible Electroporation: A Novel Image-Guided Cancer Therapy

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