A novel optical interference technique to measure minute root elongations of Japanese red pine (Pinus densiflora Seibold &amp; Zucc.) seedlings infected with ectomycorrhizal fungi

Rathnayake, Anura Pushpakumara; Kadono, Hirofumi; Toyooka, Satoru; Miwa, Makoto

doi:10.1016/j.envexpbot.2008.02.007

Cited by 16 publications

(18 citation statements)

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“…In Pinus densiflora, the ECM fungal species, Pisolithus sp. and Cenoccum geophilum, differ in their induction of root growth (Rathnayake et al, 2008).…”

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confidence: 99%

Development of the Poplar-Laccaria bicolor Ectomycorrhiza Modifies Root Auxin Metabolism, Signaling, and Response

et al. 2015

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Root systems of host trees are known to establish ectomycorrhizae (ECM) interactions with rhizospheric fungi. This mutualistic association leads to dramatic developmental modifications in root architecture, with the formation of numerous short and swollen lateral roots ensheathed by a fungal mantle. Knowing that auxin plays a crucial role in root development, we investigated how auxin metabolism, signaling, and response are affected in poplar (Populus spp.)-Laccaria bicolor ECM roots. The plant-fungus interaction leads to the arrest of lateral root growth with simultaneous attenuation of the synthetic auxin response element DR5. Measurement of auxin-related metabolites in the free-living partners revealed that the mycelium of L. bicolor produces high concentrations of the auxin indole-3-acetic acid (IAA). Metabolic profiling showed an accumulation of IAA and changes in the indol-3-pyruvic acid-dependent IAA biosynthesis and IAA conjugation and degradation pathways during ECM formation. The global analysis of auxin response gene expression and the regulation of AUXIN SIGNALING F-BOX PROTEIN5, AUXIN/IAA, and AUXIN RESPONSE FACTOR expression in ECM roots suggested that symbiosis-dependent auxin signaling is activated during the colonization by L. bicolor. Taking all this evidence into account, we propose a model in which auxin signaling plays a crucial role in the modification of root growth during ECM formation.

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“…In Pinus densiflora, the ECM fungal species, Pisolithus sp. and Cenoccum geophilum, differ in their induction of root growth (Rathnayake et al, 2008).…”

mentioning

confidence: 99%

Development of the Poplar-Laccaria bicolor Ectomycorrhiza Modifies Root Auxin Metabolism, Signaling, and Response

et al. 2015

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“…and infection of roots by two ectomycorrhizal fungi (Rathnayake et al 2008). Kobayashi and Kadono (2010) reported the effect of illumination conditions on the leaf-growth of rice crops by using SIT.…”

Section: Introductionmentioning

confidence: 99%

An optical interferometric technique for assessing ozone induced damage and recovery under cumulative exposures for a Japanese rice cultivar

et al. 2014

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Exposure to ozone (O3) causes reduction both in the growth and yield of rice (Oriza sativa L.). Commonly used Chlorophyll fluorescent measurements are not sensitive enough for short term exposure of O3 aiming an immediate assessments. Such a conventional method typically needs exposure over a few days to detect the influence. As an alternative method, we proposed a novel non-invasive, robust, real-time, optical Statistical Interferometric Technique (SIT) to measure growth at an accuracy of 0.1 nm with a commonly consumed Japanese rice cultivar, Koshihikari. In the present study, we have conducted a repetitive O3 exposure experiment for three days under three different concentrations of 0 nl l-1 (control), 120 nl l-1, and 240 nl l-1, to investigate the damage and recovery strengths. As a measure to assess the effect and recovery from three consecutive day exposures of O3, we measured the elongation rate (nm mm-1 sec-1) every 5.5 sec for 7 hours, and it revealed nanometric elongation rate fluctuations or Nanometric Intrinsic Fluctuations (NIF). Comparing the standard deviation (SD) of normalized nanometric intrinsic fluctuations (NNIF), which was normalized by that before the exposure, we found that drastic reductions under both 120 nl l-1 and 240 nl l-1 O3 concentrations. Reduction percentages were large under high O3 concentration of 240 nl l-1 indicating the possibility of irreversible effect. However exposure to 120 nl l-1 of O3 showed recovery on the 2nd and 3rd days. While SIT did reveal immediate effect based on an observation for a few hours, the visible foliar effect could be observed only after a week. Hence, the technique could provide a way for fast assessment of effect and recovery due to cumulative exposure of O3 and hence the tolerance as well as the vitality of plant.Electronic supplementary materialThe online version of this article (doi: 10.1186/2193-1801-3-89) contains supplementary material, which is available to authorized users.

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“…The nanometric fluctuations of the lengths of some plant parts (leaves and roots) change in response to environmental stresses, and these nanometer-scale changes can be monitored using SIT. 20,21 To investigate any thermal effects, which can be caused by dielectric heating due to EMR exposure, 22 the temperature of plants before and during EMR exposure was observed using thermographic images.…”

Section: Introductionmentioning

confidence: 99%

Nanometer-scale elongation rate fluctuations in theMyriophyllum aquaticum(Parrot feather) stem were altered by radio-frequency electromagnetic radiation

Senavirathna

Asaeda

Thilakarathne

et al. 2014

Plant Signaling & Behavior

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A novel optical interference technique to measure minute root elongations of Japanese red pine (Pinus densiflora Seibold & Zucc.) seedlings infected with ectomycorrhizal fungi

Cited by 16 publications

References 40 publications

Development of the Poplar-Laccaria bicolor Ectomycorrhiza Modifies Root Auxin Metabolism, Signaling, and Response

Development of the Poplar-Laccaria bicolor Ectomycorrhiza Modifies Root Auxin Metabolism, Signaling, and Response

An optical interferometric technique for assessing ozone induced damage and recovery under cumulative exposures for a Japanese rice cultivar

Nanometer-scale elongation rate fluctuations in theMyriophyllum aquaticum(Parrot feather) stem were altered by radio-frequency electromagnetic radiation

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