A Novel Ornithine Transcarbamylase Present in Mycoplasma-Infected Myeloma Cells

Matsuno, Fumihiko; Matsuzaki, Hiromitsu; Akahoshi, Yoshitaka; Yoshida, Minoru; Hata, Hiroyuki; Takao, Masaki; Takatsuki, Kiyoshi; Mori, Masataka

doi:10.1159/000468658

Cited by 5 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although one cell line, KHM-4, was reported to produce ammonia in vitro, 25 it has been clarified that ornithine transcarmanylase derived from infected mycoplasma in this cell line played a major role in the production of ammonia in culture. 31 In the only other report concerning ammonia production by a myeloma cell line, RPMI8226, 29 there was no description of mycoplasma status and ammonia production was not measured according to proliferating cell numbers. Therefore, there has been no apparent observation of ammonia production by myeloma cells in vitro so far.…”

Section: Introductionmentioning

confidence: 99%

In vitro excess ammonia production in human myeloma cell lines

et al. 1998

View full text Add to dashboard Cite

It is well known that cases with multiple myeloma reveal various clinical manifestations such as pancytopenia, hyperproteinemia, renal dysfunction, bone lesions, hypercalcemia and immunodeficiency. Recently, a few more clinical features associated with myeloma, such as salivary type hyperamylasemia and elevated serum C-reactive protein (CRP) concentration, have been reported. The elevation of CRP is thought to be related to interleukin-6 (IL-6) production by myeloma cells, because of identification of IL-6 as an autocrine and/or paracrine growth factor for myeloma cells. More recently, there have been several reports of cases with myeloma associated with hyperammonemia. This hyperammonemia is not considered to be due to liver dysfunction, because in most of these cases tests revealed normal hepatic function, and some cases showed different patterns of serum amino acid distribution than that associated with hepatic failure. However, there have been no apparent observations of ammonia production by myeloma cells. In this study, we used six human myeloma cell lines including KMS-18, which was recently established from a myeloma case associated with hyperammonemia. These lines were treated with MRA (mycoplasma removal agent) to observe ammonia production in vitro. They produced and released significantly higher levels of ammonia into culture medium than non-myeloma hematological cell lines or the HepG2 human hepatic carcinoma cell line. Although attempts to analyze the relative expression levels of the enzymes related to ammonia biosynthesis using the reverse transcriptase-polymerase chain reaction assay failed to detect any differences between these myeloma lines and other cell lines, in vitro excess ammonia production by the myeloma cells was confirmed and the relevance to clinical manifestations is discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

In vitro excess ammonia production in human myeloma cell lines

et al. 1998

View full text Add to dashboard Cite

show abstract

“…To analyze the production of ammonia by myeloma cells [30], care was taken to avoid perturbation of ammonia production caused by infected mycoplasma. Furthermore, it has been reported that ornithine transcarbamylase derived from infected mycoplasma in a myeloma cell line played a major role in the production of ammonia in culture [31].…”

Section: General Information On Cell Lines Established At Kawasaki Mementioning

confidence: 99%

Genetic and biological characterization of human myeloma cell lines: An overwiew of the lines established at Kawasaki Medical School

et al. 2000

View full text Add to dashboard Cite

Human myeloma cell lines are useful as an in vitro model for the genetic and cellular biological characterization of primary myeloma cells. We have been attempting to establish myeloma lines since 1979 for the study of the cytogenetic, molecular and cellular biological aspects of myeloma cells and to resolve the clinical problems associated with multiple myeloma. The establishment and characterization of some of these lines have been reported. Here we review the lines established at Kawasaki Medical School and discuss investigations related to the clinical manifestation of myeloma using these lines. For instance, the relation between myeloma specific chromosomal translocations, such as t(11;14)(q13;q32), t(8;14)(q24;q32), and t(4;14)(q16.3;q32.3), found in some of the cell lines and gene expression was examined.

show abstract

Can mycoplasma-mediated oncogenesis be responsible for formation of conventional renal cell carcinoma?

Pehlıvan

Önay

et al. 2005

Urology

View full text Add to dashboard Cite

A Novel Ornithine Transcarbamylase Present in Mycoplasma-Infected Myeloma Cells

Cited by 5 publications

References 14 publications

In vitro excess ammonia production in human myeloma cell lines

In vitro excess ammonia production in human myeloma cell lines

Genetic and biological characterization of human myeloma cell lines: An overwiew of the lines established at Kawasaki Medical School

Can mycoplasma-mediated oncogenesis be responsible for formation of conventional renal cell carcinoma?

Contact Info

Product

Resources

About