A novel phytase characterized by thermostability and high pH tolerance from rice phyllosphere isolated Bacillus subtilis B.S.46

Rocky-Salimi, Karim; Hashemi, Maryam; Safari, Mohammad; Mousivand, Maryam

doi:10.1016/j.jare.2016.02.003

Cited by 34 publications

(25 citation statements)

References 38 publications

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“…After 16 h incubation at 4 °C, PhyRC001 maintained its constant activity in the pH range 4.0–6.0 ( Figure 5 B). The pH range of the recombinant enzyme was consistent with the alkaline phytase property reported previously [ 16 ].…”

Section: Resultssupporting

confidence: 88%

New Bacterial Phytase through Metagenomic Prospection

2018

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Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 °C. β-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for β-propeller phytase functions could be of great benefit to biotechnology.

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Section: Resultssupporting

confidence: 88%

New Bacterial Phytase through Metagenomic Prospection

2018

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“…Corn steep liquor (CSL) is a by‐product of corn wet milling process, which contains amino acids, vitamins and minerals, and it is usually used as a nitrogen source in fermentation processes . The negative effect of CSL on phytase production can be attributed to the inhibiting effect of phosphorous and magnesium contained in CSL . In comparison to BM (2.037 U mL −1 ) and SM (4.265 U mL −1 ) cultures, addition of CM (10 gL −1 ) to the medium containing SM (10 gL −1 ), respectively, led to the 3.36‐fold and 1.08‐fold improvement in phytase production by B. subtilis K46b (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The bacterial strain B. subtilis K46b, which was previously isolated from the phyllosphere of rice plant as a potent thermo‐stable phytase producer, was grown at 30 °C on agar slopes and maintained at 4 °C as described earlier …”

Section: Methodsmentioning

confidence: 99%

“…The basal medium (BM) used for phytase production by B. subtilis K46b in submerged fermentation consisted of (g L −1 ): sodium phytate 0.2, dextrin 12, yeast extract 4, meat extract 3, MgSO 4 0.3 and 1.1 CaCl 2 at pH 7.5. Erlenmeyer flasks (100 mL) containing 25‐mL cultures were inoculated with 0.5 mL 18‐h‐old culture grown on NB and incubated in a shaker incubator at 30 °C for 48 h at 170 rpm …”

Section: Methodsmentioning

confidence: 99%

“…Cane molasses (CM), the by‐product of the sugar refinery process, containing 45–50% sugars, is one of the economical source of carbohydrate for various industrial fermentations . We have recently isolated a novel phytase from B. subtilis K46b with high optimum temperature, good thermo‐stability, and stability in low acidic to high alkaline pH, which are desired properties of an ideal phytase as an animal feed supplement/additive . This is the first report on the optimisation of phytase production by a Bacillus strain in SM and CM medium using RSM.…”

Section: Introductionmentioning

confidence: 99%

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Valorisation of untreated cane molasses for enhanced phytase production by Bacillus subtilis K46b and its potential role in dephytinisation

et al. 2016

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Dephytinization of wheat and rice bran by cross‐linked enzyme aggregates of Mucor indicus phytase: a viable prospect for food and feed industries

Venkataraman

Vaidyanathan

2022

J Sci Food Agric

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BACKGROUND Novel feeds for improved feed intake and for enhanced nutrient bioavailability have recently attracted attention. Insoluble dietary fibers, especially rice and wheat bran, have generated much interest due to their nutritional value. Incorporating insoluble dietary fiber into diets could be a viable way to maximize the feed conversion ratio. RESULTS Cross‐linked phytase aggregates (CLPA) were synthesized by precipitating enzymes followed by cross‐linking with 5 mmol L−1 glutaraldehyde, yielding 88.24 (U g−1) of enzyme load without the assistance of a proteic feeder. The epitome of the study is the dephosphorylation of wheat bran and rice bran by varying pH, enzyme concentration, and temperature. The highest inorganic phosphorus liberation by 150 U L‐‐1of free phytase was 23.72 (wheat bran) and 48.08 mg g−1 (rice bran) after 12 h of incubation. Furthermore, 150 U L‐1 of CLPA liberated 28.72 (wheat bran) and 52.08 mg g−1 (rice bran) of inorganic phosphorus with an incubation time of 12 h. CONCLUSION Thermostable free phytase was insolubilized to dephosphorylate the agro‐residue, namely, wheat bran and rice bran, to reduce the anti‐nutritional factor (the phytate content) of these insoluble dietary fibers. © 2022 Society of Chemical Industry.

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A novel phytase characterized by thermostability and high pH tolerance from rice phyllosphere isolated Bacillus subtilis B.S.46

Abstract: Graphical abstract

Cited by 34 publications

References 38 publications

New Bacterial Phytase through Metagenomic Prospection

New Bacterial Phytase through Metagenomic Prospection

Valorisation of untreated cane molasses for enhanced phytase production by Bacillus subtilis K46b and its potential role in dephytinisation

Dephytinization of wheat and rice bran by cross‐linked enzyme aggregates of Mucor indicus phytase: a viable prospect for food and feed industries

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