Valorisation of untreated cane molasses for enhanced phytase production by <i>Bacillus subtilis</i> K46b and its potential role in dephytinisation

Rocky-Salimi, Karim; Hashemi, Maryam; Safari, Mohammad; Mousivand, Maryam

doi:10.1002/jsfa.7716

Cited by 11 publications

(6 citation statements)

References 36 publications

(77 reference statements)

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“…Bacillus subtilis is a gram-positive bacterium that is considered as a GRAS (generally regarded as safe) organism by the Food and Drug Administration (FDA). The bacterium possesses properties that render it suitable for metabolic engineering to develop it as a cell factory, such as its fast growth rate and capability to grow on cheap raw material like molasses [22,23]. B. subtilis also has a broad metabolic potential, no significant bias in codon usage and a wide substrate range.…”

Section: Introductionmentioning

confidence: 99%

A regulated synthetic operon facilitates stable overexpression of multigene terpenoid pathway in Bacillus subtilis

Abdallah

Xue

Pramastya

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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The creation of microbial cell factories for sustainable production of natural products is important for medical and industrial applications. This requires stable expression of biosynthetic pathways in a host organism with favorable fermentation properties such as Bacillus subtilis. The aim of this study is to construct B. subtilis strains that produce valuable terpenoid compounds by overexpressing the innate methylerythritol phosphate (MEP) pathway. A synthetic operon allowing the concerted and regulated expression of multiple genes was developed. Up to 8 genes have been combined in this operon and a stably inherited plasmid-based vector was constructed resulting in a high production of C30 carotenoids. For this, two vectors were examined, one with rolling circle replication and another with theta replication. Theta-replication constructs were clearly superior in structural and segregational stability compared to rolling circle constructs. A strain overexpressing all eight genes of the MEP pathway on a theta-replicating plasmid clearly produced the highest level of carotenoids. The level of transcription for each gene in the operon was similar as RT-qPCR analysis indicated. Hence, that corresponding strain can be used as a stable cell factory for production of terpenoids. This is the first report of merging and stably expressing this large-size operon (eight genes) from a plasmid-based system in B. subtilis enabling high C30 carotenoid production.

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Section: Introductionmentioning

confidence: 99%

A regulated synthetic operon facilitates stable overexpression of multigene terpenoid pathway in Bacillus subtilis

Abdallah

Xue

Pramastya

et al. 2020

Journal of Industrial Microbiology and Biotechnology

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“…Sucrose in cane molasses can be hydrolyzed into monosaccharides by microbial enzymes, which is believed to be suitable for microbial growth and metabolite production [8,13]. In this study, to develop a cost-effective fermentation, PCM was used for cells growth and isomaltose synthesis.…”

Section: Resultsmentioning

confidence: 99%

“…Given that available ingredients enrich in cane molasses, nowadays, it is increasingly utilized as an alternative feedstock for microbial fermentation after a pretreatment or as the raw material. Different high value-added metabolites have been harvested via microbial fermentation from molasses, such as astaxanthin [6], 5-hydroxymethylfurfural [7], ethanol [8,9], organic acids [3,10,11,12], and enzymes [13].…”

Section: Introductionmentioning

confidence: 99%

Efficient Conversion of Cane Molasses Towards High-Purity Isomaltulose and Cellular Lipid Using an Engineered Yarrowia lipolytica Strain in Fed-Batch Fermentation

Wang

Liu

et al. 2019

Molecules

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: Cane molasses is one of the main by-products of sugar refineries, which is rich in sucrose. In this work, low-cost cane molasses was introduced as an alternative substrate for isomaltulose production. Using the engineered Yarrowia lipolytica, the isomaltulose production reached the highest (102.6 g L−1) at flask level with pretreated cane molasses of 350 g L−1 and corn steep liquor of 1.0 g L−1. During fed-batch fermentation, the maximal isomaltulose concentration (161.2 g L−1) was achieved with 0.96 g g−1 yield within 80 h. Simultaneously, monosaccharides were completely depleted, harvesting the high isomaltulose purity (97.4%) and high lipid level (12.2 g L−1). Additionally, the lipids comprised of 94.29% C16 and C18 fatty acids, were proved suitable for biodiesel production. Therefore, the bioprocess employed using cane molasses in this study was low-cost and eco-friendly for high-purity isomaltulose production, coupling with valuable lipids.

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“…Although phytases can be obtained from several sources such as animals, plants, and microbes, the latter are considered to be a potent source of phytases 22–24 . On a commercial scale, microbes can be easily propagated, and the phytase produce by them is stable and usable under different aquaculture conditions 25 . Over the last decades, considerable progress has been made to understand the effect of microbial phytases as feed additive and their impact on fish growth performance 23,26,27 .…”

Section: Introductionmentioning

confidence: 99%

“…[22][23][24] On a commercial scale, microbes can be easily propagated, and the phytase produce by them is stable and usable under different aquaculture conditions. 25 Over the last decades, considerable progress has been made to understand the effect of microbial phytases as feed additive and their impact on fish growth performance. 23,26,27 The role of phytases in improving the growth performances and nutrition in aquaculture has been documented earlier.…”

Section: Introductionmentioning

confidence: 99%

Role of microbial phytases in improving fish health

Priya

Virmani

Pragya

et al. 2023

Reviews in Aquaculture

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Fishmeal is the most preferred protein source in aquafeed industry due to its balanced amino acid and fatty acid profile, but its limited global supply and high price value, necessitates researchers to focus on sustainable alternative sources of protein and plant‐based feed, to meet the growing demand of fish feed. Phytate is a reservoir of phosphorus present in the plants; however, its chelating ability makes it anti‐nutritional. Phytate forms complexes with minerals, proteins, lipids and carbohydrates, confining their accessibility in fish feeds, hence, additional dietary nutrients are required in the fish feed to overcome nutritional deficiencies. The elimination of phytate‐phosphorus through animal faeces and its pass on to aquatic bodies leads to enrichment of nutrients, eutrophication and algal blooms. Microbial phytase segregates phosphorus, minerals, proteins and lower inositol phosphates from phytate. The supplementation of fish feed with microbial phytase enhances the digestibility of minerals, due to which less nutrients are excreted in the faeces; therefore, minimizing pollution in water bodies. Monogastric and agastric animals inherently exhibit the deficiency of phytase; hence, supplementation of dietary material with phytase has been an emerging choice to make dietary nutrients available for fish. Moreover, microbial phytases have a broad pH range and are thermostable, which makes them an excellent choice for commercial fish feed production. Additionally, microbial phytase improves fish growth as these enzymes enhance nutrients bioavailability and their digestion. This review article presents the application of microbial phytases as a feed additive, and their role in enhancing fish growth.

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Valorisation of untreated cane molasses for enhanced phytase production by Bacillus subtilis K46b and its potential role in dephytinisation

Cited by 11 publications

References 36 publications

A regulated synthetic operon facilitates stable overexpression of multigene terpenoid pathway in Bacillus subtilis

A regulated synthetic operon facilitates stable overexpression of multigene terpenoid pathway in Bacillus subtilis

Efficient Conversion of Cane Molasses Towards High-Purity Isomaltulose and Cellular Lipid Using an Engineered Yarrowia lipolytica Strain in Fed-Batch Fermentation

Role of microbial phytases in improving fish health

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