Dark current limits the optical performance of CMOS image sensors. The main sources of the dark current in a modern submicron process are the defects induced by the shallow trench isolation fabrication process steps. In this paper, we present a pixel layout technique to reduce the impact of these defects by removing the trench-oxide between the two adjacent edges of neighbouring photodiodes. This isolation scheme relies on the p-well layer only and provides the further advantage of requiring less area. Hence, a larger photodiode can be designed, leading to an increased pixel fill factor. Experimental results show that this approach reduces the dark current by 21% and increases the linear full well capacity by approximately 9%.