A Novel Polyphenol Oxidoreductase OhLac from Ochrobactrum sp. J10 for Lignin Degradation

Yang, Chenxian; Ma, Lingling; Wang, Xin; Xing, Yuqi; Lü, Xin

doi:10.3389/fmicb.2021.694166

Cited by 4 publications

(2 citation statements)

References 58 publications

(60 reference statements)

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“…In addition, a short generation time of bacteria makes it easier to scale up laccase production processes on a commercial scale (Brugnari et al, 2021 ; Akram et al, 2022 ). The major bacterial genera that have been reported to produce laccase-like multicopper oxidases include Streptomyces, Bacillus, Meiothermus, Gramella, Geobacillus, Aquisalibacillus, Lysinibacillus, Azospirillum, Rhodococcus, Ochrobactrum, Amycolatopsis, Pseudomonas, Stenotrophomonas, Iodidimonas, Alteromonas, and Nitrosomonas (Chauhan et al, 2017 ; Granja-Travez et al, 2018 ; Arregui et al, 2019 ; Jeon and Park, 2020 ; Yang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a New Serratia proteamaculans Strain That Naturally Produces Significant Amount of Extracellular Laccase

et al. 2022

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Natural biodegradation processes hold promises for the conversion of agro-industrial lignocellulosic biomaterials into biofuels and fine chemicals through lignin-degrading enzymes. The high cost and low stability of these enzymes remain a significant challenge to economic lignocellulosic biomass conversion. Wood-degrading microorganisms are a great source for novel enzyme discoveries. In this study, the decomposed wood samples were screened, and a promising γ-proteobacterial strain that naturally secreted a significant amount of laccase enzyme was isolated and identified as Serratia proteamaculans AORB19 based on its phenotypic and genotypic characteristics. The laccase activities in culture medium of strain AORB19 were confirmed both qualitatively and quantitatively. Significant cultural parameters for laccase production under submerged conditions were identified following a one-factor-at-a-time (OFAT) methodology: temperature 30°C, pH 9, yeast extract (2 g/l), Li+, Cu2+, Ca2+, and Mn2+ (0.5 mM), and acetone (5%). Under the selected conditions, a 6-fold increase (73.3 U/L) in laccase production was achieved when compared with the initial culturing conditions (12.18 U/L). Furthermore, laccase production was enhanced under alkaline and mesophilic growth conditions in the presence of metal ions and organic solvents. The results of the study suggest the promising potential of the identified strain and its enzymes in the valorization of lignocellulosic wastes. Further optimization of culturing conditions to enhance the AORB19 strain laccase secretion, identification and characterization of the purified enzyme, and heterologous expression of the specific enzyme may lead to practical industrial and environmental applications.

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Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a New Serratia proteamaculans Strain That Naturally Produces Significant Amount of Extracellular Laccase

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In addition, a short generation time of bacteria makes it easier to scale up laccase production processes on a commercial scale (Brugnari et al, 2021;Akram et al, 2022). The major bacterial genera that have been reported to produce laccase-like multicopper oxidases include Streptomyces, Bacillus, Meiothermus, Gramella, Geobacillus, Aquisalibacillus, Lysinibacillus, Azospirillum, Rhodococcus, Ochrobactrum, Amycolatopsis, Pseudomonas, Stenotrophomonas, Iodidimonas, Alteromonas, and Nitrosomonas (Chauhan et al, 2017;Granja-Travez et al, 2018;Arregui et al, 2019;Jeon and Park, 2020;Yang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%