A novel polysaccharide from the Sarcodon aspratus triggers apoptosis in Hela cells via induction of mitochondrial dysfunction

Wang, Dandan; Wu, Qing-Xi; Pan, Wen-Juan; Hussain, Sajid; Mehmood, Shomaila; Chen, Yan

doi:10.29219/fnr.v62.1285

Cited by 22 publications

(15 citation statements)

References 30 publications

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“…The plates with B. cereus and S. typhimurium were incubated at 37°C for 24 h. The suspension in each well was removed and the microtiter wells were washed for four times with PBS to remove non-adherent cells. Biofilms were stained with MTT solution (prepared with the corresponding medium) and incubated in dark for 3 h at 37°C (34,35). Dimethyl sulfoxide (DMSO) was used to rinse after removal of MTT solution.…”

Section: Inhibition Activitymentioning

confidence: 99%

A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro

Xu¹,

Peng²,

Zhang³

et al. 2020

Food & Nutrition Research

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Section: Inhibition Activitymentioning

confidence: 99%

A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro

Xu¹,

Peng²,

Zhang³

et al. 2020

Food & Nutrition Research

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“…In recent years, various therapeutic means have contributed to the treatment and prevention of cancers, but these methods can cause irreversible side effects. Thus, the immediate task of the new drug research is to pursue the natural alternatives with high therapeutic properties and low toxicity (35). Pholidonone is a natural antitumor stilbene derivative isolated from EtOAc extract of P. cantonensis .…”

Section: Resultsmentioning

confidence: 99%

Pholidonone, an active stilbene derivative from Pholidota cantonensis, exhibits pro-apoptotic effect via induction of endoplasmic reticulum stress in human gastric cancer

Liu¹,

Wang²,

Zhao³

et al. 2019

Food & Nutrition Research

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BackgroundGastric cancer (GC) is the second leading cause of cancer death worldwide. Current chemotherapeutic drugs exert therapeutic effects accompanied by severe side effects. Therefore, it is imperative to urgently find new drugs with low toxicity and high efficacy for the treatment of GC. Natural products as well as functional foods have always been rich sources of potential antitumor agents. Pholidota cantonensis Rolfe, a well-known functional food and a traditional Chinese medicine, has been used for a long time in China for inflammatory diseases. Previously, we have evaluated its possible antitumor potentials by screening different solvent extracts, and found that the ethyl acetate (EtOAc) extract showed potent cytotoxicity on human GC cell line AGS with an IC50 value of 33.68 ± 1.68 μg/mL. In view of the poor knowledge concerning the phytochemical and pharmacological study of P. cantonensis, it is essential to characterize the active compounds from EtOAc extract and the mechanisms of action underlying the antitumor effect of the herb.ObjectiveThis study aimed to identify the primary compounds in EtOAc extract of P. cantonensis involved in the antitumor activity of the plant by evaluating the cytotoxicity in two human GC cell lines, including AGS and BGC-823 cells. Since endoplasmic reticulum (ER) stress-induced cell apoptosis represents attractive targets for cancer therapy recently, we focused on the underlying mechanisms associated with ER stress-induced cell apoptosis and related signaling pathways.MethodsVarious chromatographic techniques, including silica gel, Sephadex LH-20, and octadecylsilyl silica gel (ODS) C18, were used to separate the main active compound from EtOAc extract of P. cantonensis. The cell viability of AGS and BGC-823 cells upon purified compound treatment was determined by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The alteration of cell morphology was observed using an inverted microscope. Cell apoptosis was determined by fluorescein isothiocyanate (FITC)-labeled annexin-V/propidium iodide (PI) double-staining and flow cytometry analysis. Western blot analyses were performed to examine the levels of intracellular signaling molecules involved in ER stress-induced apoptosis.ResultsA rare stilbene derivative pholidonone was isolated and identified. The results showed that pholidonone displayed potent cytotoxicity on human GC cells. The IC50 values for 24 and 48 h in AGS cells were 26.54 ± 0.32 and 25.20 ± 3.67 μM, and the IC50 values for 24 and 48 h in BGC-823 cells were 32.41 ± 3.83 and 17.28 ± 2.30 μM, respectively. In addition, pholidonone had pro-apoptotic effect on AGS and BGC-823 cells, and it upregulated the levels of proteins involved in ER stress, including BiP, PDI, Calnexin, Ero1-Lα, IRE1α, PERK, CHOP, and cleaved-caspase-3 in AGS and BGC-823 cells.ConclusionPholidonone can trigger ER stress-induced apoptosis through PERK and IRE1α signaling pathways. Pholidonone might be a potential naturally occurring antitumor agent.

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“…It has been reported that a number of components of CHM can inhibit the growth of tumor cells both in vitro and in vivo, such as polysaccharides, flavones, terpenoids, and phenols [ 21 , 22 , 23 , 24 , 25 , 26 ]. The components of polysaccharides, flavones, and triterpenes in AAEE, AAEE-Pe, and AAEE-Ea were quantified.…”

Section: Discussionmentioning

confidence: 99%

The Extracts of Artemisia absinthium L. Suppress the Growth of Hepatocellular Carcinoma Cells through Induction of Apoptosis via Endoplasmic Reticulum Stress and Mitochondrial-Dependent Pathway

Wei

Ziyayiding

et al. 2019

Molecules

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Artemisia absinthium L. has pharmaceutical and medicinal effects such as antimicrobial, antiparasitic, hepatoprotective, and antioxidant activities. Here, we prepared A. absinthium ethanol extract (AAEE) and its subfractions including petroleum ether (AAEE-Pe) and ethyl acetate (AAEE-Ea) and investigated their antitumor effect on human hepatoma BEL-7404 cells and mouse hepatoma H22 cells. The cell viability of hepatoma cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis, cell cycle, mitochondrial membrane potential (Δψm), and reactive oxygen species (ROS) were analyzed by flow cytometry. The levels of proteins in the cell cycle and apoptotic pathways were detected by Western blot. AAEE, AAEE-Pe, and AAEE-Ea exhibited potent cytotoxicity for both BEL-7404 cells and H22 cells through the induction of cell apoptosis and cell cycle arrest. Moreover, AAEE, AAEE-Pe, and AAEE-Ea significantly reduced Δψm, increased the release of cytochrome c, and promoted the cleavage of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP) in BEL-7404 and H22 cells. AAEE, AAEE-Pe, and AAEE-Ea significantly upregulated the levels of ROS and C/EBP-homologous protein (CHOP). Further, AAEE, AAEE-Pe, and AAEE-Ea significantly inhibited tumor growth in the H22 tumor mouse model and improved the survival of tumor mice without side effects. These results suggest that AAEE, AAEE-Pe, and AAEE-Ea inhibited the growth of hepatoma cells through induction of apoptosis, which might be mediated by the endoplasmic reticulum stress and mitochondrial-dependent pathway.

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A novel polysaccharide from the Sarcodon aspratus triggers apoptosis in Hela cells via induction of mitochondrial dysfunction

Cited by 22 publications

References 30 publications

A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro

A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro

Pholidonone, an active stilbene derivative from Pholidota cantonensis, exhibits pro-apoptotic effect via induction of endoplasmic reticulum stress in human gastric cancer

The Extracts of Artemisia absinthium L. Suppress the Growth of Hepatocellular Carcinoma Cells through Induction of Apoptosis via Endoplasmic Reticulum Stress and Mitochondrial-Dependent Pathway

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