A novel probiotic strain, Lactiplantibacillus plantarum LC38, isolated from Tunisian camel milk promoting wound healing in Wistar diabetic rats

Abstract

Antimicrobial and probiotic properties were sought in Tunisian raw camel milk. One hundred and forty strains were isolated for this purpose. Initially, a screening based on growth kinetics under gastrointestinal tract (GIT) conditions allowed us to select only one strain that showed good resistance to pH 3 and 5% bovine bile. This isolate named LC38 was assayed for its autoaggregation capacity, surface hydrophobicity, antibiotic sensitivity, haemolytic and antimicrobial activities. The findings revealed strain LC38 sensitivity to several antibiotics, no haemolytic activity and antimicrobial activities against six pathogenic bacteria with an inhibition diameter that varies between 28 and 43 mm. Furthermore, this study revealed that this strain had good autoaggregation characteristics after 18 h of incubation and a high surface hydrophobicity that enhanced its adhesion ability to epithelial cells and for biofilm formation. Strain LC38 was submitted to classical identification with API50CH and to 16S rRNA gene sequencing, which revealed that the strain could be allocated to Lactiplantibacillus plantarum. Analysis of the structural composition of strain LC38 by high-performance liquid chromatography (HPLC) and Fourier transform-infrared (FTIR) spectroscopy analysis showed that probiotic suspension contains organic acids (lactic acid, succinic acid and citric acids). The application of Lactiplantibacillus plantarum LC38 on wound site in a diabetic rat model enhanced significantly wound healing activity and accelerated the wound closure after 14 days of wound induction. Altogether, these results demonstrated that LC38 isolate from camel milk has a number of promising properties that make it a prominent candidate with strong wound healing potential presumably achieved through its antimicrobial activities.

Probiotic-Functionalized Silk Fibroin/Sodium Alginate Scaffolds with Endoplasmic Reticulum Stress-Relieving Properties for Promoted Scarless Wound Healing

“…Probiotics showed beneficial effects in wound healing, which might be attributed to the ability to secrete lactic acid . In this study, HPLC analysis showed that L-SF/SA scaffolds could bring in lactic acid in the wound.…”

“…Probiotics showed beneficial effects in wound healing, which might be attributed to the ability to secrete lactic acid. 28 In this study, HPLC analysis showed that L-SF/SA scaffolds could bring in lactic acid in the wound. Increasing numbers of studies have found that lactic acid played different physiological roles as an activity signal in various biological processes.…”

Type 2 diabetes and succinate: unmasking an age-old molecule

“…Meanwhile, Blautia wexlerae , also a succinate producer, counteracted obesity and diabetes induced by an HFD, with succinate levels reported to increase primarily in adipose tissue and the liver [ 19 ]. Focusing on diabetes complications, daily topical application of the beneficial bacterium Lactiplantibacillus plantarum was found to produce succinate and expedite wound healing in rat models of type 1 diabetes [ 79 ]. It is crucial to emphasise, however, that none of these studies have conclusively established that the observed beneficial effects are solely attributable to succinate.…”

Evaluating Enterococcus faecium9 N‐2 as a probiotic candidate from traditional village white cheese

“…A successful probiotic strain should be nontoxic or nonpathogenic, resistant to stomach acidity and bile salts, able to settle and multiply in the gastrointestinal tract, increase the digestibility of nutrients, and create positive effects on health (Chouikhi et al., 2022 ; Somashekaraiah et al., 2019 ). Candidate bacterial strains should therefore be thoroughly analyzed for safety and probiotic characteristics.…”