A novel process for isolation and purification of the bioactive polysaccharide TLH-3′ from Tricholoma lobayense

Liu, Liu; Lu, Yongming; Li, Xuehui; Zhou, Liyuan; Yang, Diya; Wang, Liming; Chen, Yan

doi:10.1016/j.procbio.2015.04.011

Cited by 32 publications

(8 citation statements)

References 19 publications

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“…The monosaccharide compositions of the polysaccharide were determined using the method reported by our laboratory ( 12 ). Briefly, the SAP (10 mg) was hydrolyzed with 2 M trifluoroacetic acid (TFA, 110°C, 6 h), and the residual acid was removed with a rotary evaporator at 65°C under vacuum.…”

Section: Methodsmentioning

confidence: 99%

A novel polysaccharide from the Sarcodon aspratus triggers apoptosis in Hela cells via induction of mitochondrial dysfunction

Wang¹,

Wu²,

Pan³

et al. 2018

Food & Nutrition Research

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BackgroundPolysaccharides extracted from fungus that have been used widely in the food and drugs industries due to biological activities.ObjectiveThe objective of the present study was to investigate the tumor-suppressive activity and mechanism of a novel polysaccharide (SAP) extracted from Sarcodon aspratus.MethodsThe SAP was extracted and purified using Sepharose CL-4B gel from S. aspratus. The cytotoxicity of SAP on cell lines was determined by MTT method. Cellular migration assays were implemented by using transwell plates. The apoptosis and mitochondrial membrane potential (Δψm) of Hela cells were analyzed by flow cytometry. The western blot was used to determine the protein expression of Hela cells.ResultsThe results showed that SAP with a molecular weight of 9.01×105 Da could significantly inhibit the growth of Hela cells in vitro. Three-dimensional cell culture (3D) and transwell assays showed that SAP restrained the multi-cellular spheroids growth and cell migration. Flow cytometry analysis revealed that SAP induced a loss of mitochondrial membrane potential (Δψm). Western blot assays indicated that SAP promoted the release of cytochrome c, increased Bax expression, down-regulated of Bcl-2 expression and activated of caspase-3 expression.ConclusionThis study suggested that SAP induced Hela cells apoptosis via mitochondrial dysfunction that are critical in events of caspase apoptotic pathways. The anti-tumor (Hela cells) activity of SAP recommended that S. aspratus could be used as a powerful medicinal mushroom against cancer.

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Section: Methodsmentioning

confidence: 99%

A novel polysaccharide from the Sarcodon aspratus triggers apoptosis in Hela cells via induction of mitochondrial dysfunction

Wang¹,

Wu²,

Pan³

et al. 2018

Food & Nutrition Research

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“…Ultrafiltration is more commonly used to remove small molecules such as inorganic salts and oligosaccharides. If the diameter of the solute molecules is larger than the pore size of the ultrafiltration membrane, they will be blocked and remain under the ultrafiltration membrane; conversely, solute molecules may be retained or adsorbed on the surface of the ultrafiltration membrane (L. Liu et al, 2015). Du et al (2010) selected an ultrafiltration system equipped with 0.1 m 2 ultrafiltration membranes with molecular weights of 500, 100, and 10 kDa for ultrafiltration separation, Three crude White jellyfungus polysaccharide fractions were obtained, namely TAP50w, TAP10‐50w, and TAP1‐10w (Du et al, 2010).…”

Section: Purification and Separationmentioning

confidence: 99%

Extraction, isolation, purification, derivatization, bioactivity, structure–activity relationship, and application of polysaccharides from White jellyfungus

Lin

Huang

2022

Biotech & Bioengineering

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White jellyfungus is one of the most popular nutritional supplements. The polysaccharide (WJP) is an important active component of White jellyfungus, it not only has a variety of biological activities but also is nontoxic to humans. So, many scholars have carried out different research on WJP. However, the lack of a detailed summary of WJP limits the scale of industrial development of WJP.Herein, the research progress of WJP in extraction, isolation, structure, derivatization, and structure-activity relationship was reviewed. Different extraction methods were compared, the activity and application of WJP were summarized, and the structure-activity relationship of WJP was emphasized to provide effective theoretical support for improving the utilization of WJP and promoting the application of related industries.

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“…A sample of the polysaccharide was redissolved in distilled water, and then purified with the DEAEcellulose A52 column (2.6 cm×60 cm), which was equilibrated with distilled water. The polysaccharide was fractionated by stepwise elution with distilled water (Gabriela, et al, 2003;Liu, Lu, et al 2015). Polysaccharide containing fractions were determined for the presence of the total sugar by phenol-sulfuric acid method (Dubois et al, 1956).…”

Section: Purification Of Eps By Diethylaminoethyl-cellulose (Deaecellulose)mentioning

confidence: 99%

THE ROLE OF EXOPOLYSACCHARIDE PRODUCED BY Pleurotus Sapidus AS MEDICAL AGENT

El-Zaher

Zied

Mohamed

et al. 2021

Delta Journal of Science

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Mushrooms have been used since centuries for the treatment of different diseases and efforts were made to isolate different compounds from the mushrooms responsible for medicinal activities. Among the mushroom genus Pleurotus is worldwide in distribution due to their medicinal properties such interventions ameliorate hepatic lipid profiles, reduce inflammation and oxidative stress, noticeable antioxidant, antiinflammatory, antimicrobial, antitumor and analgesic properties. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. The aim of current study is to improve of Exopolysaccharide production by Pleurotus sapidus through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; shaking, starch and incubation temperature are the most effective parameters. Box-Behnken RSM indicated that; the optimum concentration for each parameter was: 148.9 rpm for shaking, 30.1g/l starch and 25.2 ºC for incubation temperature. After production, EPS was extracted, purified, identified using Fourier transform infrared (FTIR), Nuclear Magnetic Resonance (¹HNMR) and High Performance Liquid Chromatography (HPLC). The result indicated that; it consisting of β-(1→3) glucans, β-(1→6) glucans branched, Chitin, Mannans and Galactans with different concentrations in EPS. Exopolysaccharides extracted from Pleurotus sapidus showed antitumor, antiviral, antibacterial, anticoagulant and antioxidant activity.

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A novel process for isolation and purification of the bioactive polysaccharide TLH-3′ from Tricholoma lobayense

Cited by 32 publications

References 19 publications

A novel polysaccharide from the Sarcodon aspratus triggers apoptosis in Hela cells via induction of mitochondrial dysfunction

A novel polysaccharide from the Sarcodon aspratus triggers apoptosis in Hela cells via induction of mitochondrial dysfunction

Extraction, isolation, purification, derivatization, bioactivity, structure–activity relationship, and application of polysaccharides from White jellyfungus

THE ROLE OF EXOPOLYSACCHARIDE PRODUCED BY Pleurotus Sapidus AS MEDICAL AGENT

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