A novel R2R3-MYB transcription factor LlMYB305 from Lilium longiflorum plays a positive role in thermotolerance via activating heat-protective genes

Wu, Ze; Li, Ting; Yuan, Guozhen; Hou, Huizhong; Teng, Nianjun

doi:10.1016/j.envexpbot.2021.104399

Cited by 29 publications

(19 citation statements)

References 45 publications

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“…Transformants were grown on SD/‐Trp‐Ura drop‐out plates supplemented with X‐gal (5‐bromo‐4‐chloro‐3‐indolyl β‐ d ‐galactoside), development of blue color indicate an interaction. β‐Galactosidase activities were measured as described by Wu et al (2021). The three tandem CRE of the HD‐Zip I proteins, named as 3 × HDZIB, was also used as a control in yeast.…”

Section: Methodsmentioning

confidence: 99%

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Genome‐wide characterization of homeodomain‐leucine zipper genes reveals RsHDZ17 enhances the heat tolerance in radish (Raphanus sativus L.)

Wang

et al. 2022

Physiologia Plantarum

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Homeodomain-leucine zipper (HD-Zip) transcription factors are involved in various biological processes of plant growth, development, and abiotic stress response.However, how they regulate heat stress (HS) response remains largely unclear in plants. In this study, a total of 83 RsHD-Zip genes were firstly identified from the genome of Raphanus sativus. RNA-Seq, RT-qPCR and promoter activity assays revealed that RsHDZ17 from HD-Zip Class I was highly expressed under heat, salt, and Cd stresses. RsHDZ17 is a nuclear protein with transcriptional activity at the C-terminus.Ectopic overexpression (OE) of RsHDZ17 in Arabidopsis thaliana enhanced the HS tolerance by improving the survival rate, photosynthesis capacity, and scavenging for reactive oxygen species (ROS). In addition, transient OE of RsHDZ17 in radish cotyledons impeded cell injury and augmented ROS scavenging under HS. Moreover, yeast one-hybrid, dual-luciferase assay, and electrophoretic mobility shift assay revealed that RsHDZ17 could bind to the promoter of HSFA1e. Collectively, these pieces of evidence demonstrate that RsHDZ17 could play a positive role in thermotolerance, partially through up-regulation of the expression of HSFA1e in plants. These results provide novel insights into the role of HD-Zips in radish and facilitate genetical engineering and development of heat-tolerant radish in breeding programs.

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Section: Methodsmentioning

confidence: 99%

“…These vectors were transformed into A. tumefaciens strain GV3101 (pSoup). The LUC fluorescence imaging and LUC/REN ratio were conducted as described previously (Fan et al, 2020; Wu et al, 2021). All primers used for this assay are listed in Table S2.…”

Section: Methodsmentioning

confidence: 99%

Genome‐wide characterization of homeodomain‐leucine zipper genes reveals RsHDZ17 enhances the heat tolerance in radish (Raphanus sativus L.)

Wang

et al. 2022

Physiologia Plantarum

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“…Lily (Lilium spp.) is an important ornamental crop for production of cut and potted flowers and for application as groundcover in landscaping [1][2][3][4]. In addition, lily bulbs are an extremely important food and medicinal material in China.…”

Section: Introductionmentioning

confidence: 99%

Starch Degradation and Sucrose Accumulation of Lily Bulbs after Cold Storage

et al. 2022

IJMS

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Functional lilies are a group of edible lily cultivars with great potential for landscape application. Low-temperature storage can significantly improve their taste, but the knowledge of this process is largely unknown. In this study, we used the functional lilies ‘Fly Shaohua’ and ‘Fly Tiancheng’ as materials. Through physiological observation and transcriptome analysis during the bulbs’ cold storage, it was found that the starch degradation and sucrose accumulation in bulbs contributed to taste improvement. After 60 d of cold storage, the sucrose accumulation was highest and the starch content was lower in the bulbs, suggesting this time-point was optimal for consumption. Accompanying the fluctuation of sucrose content during cold storage, the enzyme activities of sucrose phosphate synthase and sucrose synthase for sucrose synthesis were increased. Transcriptome analysis showed that many differentially expressed genes (DEGs) were involved in the starch and sucrose metabolism pathway, which might promote the conversion of starch to sucrose in bulbs. In addition, the DEGs involved in dormancy and stress response were also determined during cold storage, which might explain the decreased sucrose accumulation with extended storage time over 60 d due to the energy consumption for dormancy release. Taken together, our results indicated sucrose accumulation was a main factor in the taste improvement of lily bulbs after cold storage, which is attributable to the different gene expression of starch and sucrose metabolism pathways in this process.

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“…A large number of studies have shown that plant MYB TFs are widely involved in the response to abiotic stress, such as drought (Huang et al, 2018;Alexander et al, 2019;Fang et al, 2019), heat (Li J. et al, 2021;Wu et al, 2021), cold (Gong et al, 2018), and salt (Zhao K. et al, 2020), and the regulation of the biosynthesis of various secondary metabolites, such as glucosinolates (Dubos et al, 2010), flavonoids (Kortstee et al, 2011;Anwar et al, 2019;Li Y. et al, 2020) and terpenoids (Matías-Hernández et al, 2017). In addition, MYB TFs are involved in the regulation of plant cell morphology and pattern developing (Lai et al, 2005;Guimil and Dunand, 2006) and the regulation of multiple growth and development processes (Byrne et al, 2000;Wang et al, 2009;Zhuang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

PpMYB52 negatively regulates peach bud break through the gibberellin pathway and through interactions with PpMIEL1

Zhang

Tan²,

Wang³

et al. 2022

Front. Plant Sci.

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Bud dormancy, which enables damage from cold temperatures to be avoided during winter and early spring, is an important adaptive mechanism of deciduous fruit trees to cope with seasonal environmental changes and temperate climates. Understanding the regulatory mechanism of bud break in fruit trees is highly important for the artificial control of bud break and the prevention of spring frost damage. However, the molecular mechanism underlying the involvement of MYB TFs during the bud break of peach is still unclear. In this study, we isolated and identified the PpMYB52 (Prupe.5G240000.1) gene from peach; this gene is downregulated in the process of bud break, upregulated in response to ABA and downregulated in response to GA. Overexpression of PpMYB52 suppresses the germination of transgenic tomato seeds. In addition, Y2H, Bimolecular fluorescence complementation (BiFC) assays verified that PpMYB52 interacts with a RING-type E3 ubiquitin ligase, PpMIEL1, which is upregulated during bud break may positively regulate peach bud break by ubiquitination-mediated degradation of PpMYB52. Our findings are the first to characterize the molecular mechanisms underlying the involvement of MYB TFs in peach bud break, increasing awareness of dormancy-related molecules to avoid bud damage in perennial deciduous fruit trees.

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A novel R2R3-MYB transcription factor LlMYB305 from Lilium longiflorum plays a positive role in thermotolerance via activating heat-protective genes

Cited by 29 publications

References 45 publications

Genome‐wide characterization of homeodomain‐leucine zipper genes reveals RsHDZ17 enhances the heat tolerance in radish (Raphanus sativus L.)

Genome‐wide characterization of homeodomain‐leucine zipper genes reveals RsHDZ17 enhances the heat tolerance in radish (Raphanus sativus L.)

Starch Degradation and Sucrose Accumulation of Lily Bulbs after Cold Storage

PpMYB52 negatively regulates peach bud break through the gibberellin pathway and through interactions with PpMIEL1

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