A Novel R2R3-MYB Transcription Factor SbMYB12 Positively Regulates Baicalin Biosynthesis in Scutellaria baicalensis Georgi

Wang, Wentao; Hu, Suying; Yang, Jing; Zhang, Caijuan; Zhang, Tong; Wang, Dong-Hao; Cao, Xiaoyan; Wang, Zhezhi

doi:10.3390/ijms232415452

Cited by 14 publications

(4 citation statements)

References 51 publications

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“…SiMYB75 positively regulates the responses to drought, salt, and osmotic stress through an ABA-mediated pathway [83]. TcMYB8 and SbMYB12 can respond to MeJA treatment [84,85], and GlMYB4 and GlMYB88 play important roles in the synthesis of flavonoids mediated using MeJA signaling [86]. In this study, we found that regulatory elements associated with MeJA and ABA were most common in EfMYB; thus, we also analyzed the expression characteristics of the nine EfMYB genes after ABA and MeJA treatments.…”

Section: Discussionmentioning

confidence: 88%

Genome-Wide Identification and Expression Pattern of MYB Family Transcription Factors in Erianthus fulvus

Rao,

Qian,

Xie

et al. 2023

Genes

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MYB family genes have many functions and are widely involved in plant abiotic-stress responses. Erianthus fulvus is an important donor material for stress-resistance genes in sugarcane breeding. However, the MYB family genes in E. fulvus have not been systematically investigated. In this study, 133 EfMYB genes, including 48 Ef1R-MYB, 84 EfR2R3-MYB and 1 Ef3R-MYB genes, were identified in the E. fulvus genome. Among them, the EfR2R3-MYB genes were classified into 20 subgroups. In addition, these EfMYB genes were unevenly distributed across 10 chromosomes. A total of 4 pairs of tandemly duplicated EfMYB genes and 21 pairs of segmentally duplicated EfMYB genes were identified in the E. fulvus genome. Protein-interaction analysis predicted that 24 EfMYB proteins had potential interactions with 14 other family proteins. The EfMYB promoter mainly contains cis-acting elements related to the hormone response, stress response, and light response. Expression analysis showed that EfMYB39, EfMYB84, and EfMYB124 could be significantly induced using low-temperature stress. EfMYB30, EfMYB70, EfMYB81, and EfMYB101 responded positively to drought stress. ABA treatment significantly induced EfMYB1, EfMYB30, EfMYB39, EfMYB84, and EfMYB130. All nine genes were induced using MeJA treatment. These results provide comprehensive information on EfMYB genes and can serve as a reference for further studies of gene function.

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Section: Discussionmentioning

confidence: 88%

Genome-Wide Identification and Expression Pattern of MYB Family Transcription Factors in Erianthus fulvus

Rao,

Qian,

Xie

et al. 2023

Genes

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“…Baicalin biosynthesis and accumulation in S. baicalensis are known to be tightly regulated by various internal and external signals, although the underlying mechanisms remain largely unclear ( Wang et al., 2022b ). Among these signals, JA and its derivatives are plant stress hormones that play key roles in regulating flavonoid biosynthesis, root growth, and defense against pathogen infection and insect attack ( Zhu et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

SbWRKY75- and SbWRKY41-mediated jasmonic acid signaling regulates baicalin biosynthesis

et al. 2023

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IntroductionScutellaria baicalensis Georgi is a traditional Chinese medicinal plant with broad pharmacological activities whose main active ingredient is the flavonoid baicalin. Given its medicinal value and increasing market demand, it is essential to improve the plant’s baicalin content. Flavonoid biosynthesis is regulated by several phytohormones, primarily jasmonic acid (JA).MethodsIn this study, we conducted transcriptome deep sequencing analysis of S. baicalensis roots treated with methyl jasmonate for different durations (1, 3, or 7 hours). Leveraging weighted gene co-expression network analysis and transcriptome data, we identified candidate transcription factor genes involved in the regulation of baicalin biosynthesis. To validate the regulatory interactions, we performed functional assays such as yeast one-hybrid, electrophoretic mobility shift, and dual-luciferase assays.ResultsOur findings demonstrated that SbWRKY75 directly regulates the expression of the flavonoid biosynthetic gene SbCLL-7, whereas SbWRKY41 directly regulates the expression of two other flavonoid biosynthetic genes, SbF6H and SbUGT, thus regulating baicalin biosynthesis. We also obtained transgenic S.baicalensis plants by somatic embryo induction and determined that overexpressing SbWRKY75 increased baicalin content by 14%, while RNAi reduced it by 22%. Notably, SbWRKY41 indirectly regulated baicalin biosynthesis by modulating the expression of SbMYC2.1, SbJAZ3 and SbWRKY75.DiscussionThis study provides valuable insights into the molecular mechanisms underlying JA-mediated baicalin biosynthesis in S. baicalensis. Our results highlight the specific roles of transcription factors, namely SbWRKY75 and SbWRKY41, in the regulation of key biosynthetic genes. Understanding these regulatory mechanisms holds significant potential for developing targeted strategies to enhance baicalin content in S. baicalensis through genetic interventions.

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“…In a previous study, Sb MYB3, functioning as a positive regulator, was shown to bind to the promoter of Sb FNSII-2, thereby promoting RSF biosynthesis in S. baicalensis [ 22 ]. Sb MYB12, a nuclear-localized transcription activator that binds to the Sb CCL7-4, Sb CHI-2, and Sb F6H-1 promoters, positively regulates the generation of baicalin and wogonoside [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Multi-omics landscape to decrypt the distinct flavonoid biosynthesis of Scutellaria baicalensis across multiple tissues

Guo,

Zhu,

Wang

et al. 2023

Horticulture Research

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Scutellaria baicalensis Georgi, also known as Huang-Qin in Traditional Chinese Medicine, is a widely used herbal remedy due to its anticancer, antivirus, and hepatoprotective properties. The S. baicalensis genome was sequenced many years ago, by contrast, the proteome as the executer of most biological processes of S. baicalensis in the aerial parts, as well as the secondary structure of the roots (xylem, phloem and periderm) is far less comprehensively characterized. Here, we attempt to depict the molecular landscape of the non-model plant S. baicalensis through a multi-omics approach, with the goal of constructing a highly informative and valuable reference dataset. Furthermore, we provide an in-depth characterization dissection to explain two distinct flavonoid biosynthesis pathways existed in aerial part and root, respectively, at the protein and phosphorylated protein level. Our study provides detailed spatial proteomic and phosphoproteomic information in the context of secondary structures, with implications for the molecular profiling of secondary metabolite biosynthesis in non-model medicinal plants.

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A Novel R2R3-MYB Transcription Factor SbMYB12 Positively Regulates Baicalin Biosynthesis in Scutellaria baicalensis Georgi

Cited by 14 publications

References 51 publications

Genome-Wide Identification and Expression Pattern of MYB Family Transcription Factors in Erianthus fulvus

Genome-Wide Identification and Expression Pattern of MYB Family Transcription Factors in Erianthus fulvus

SbWRKY75- and SbWRKY41-mediated jasmonic acid signaling regulates baicalin biosynthesis

Multi-omics landscape to decrypt the distinct flavonoid biosynthesis of Scutellaria baicalensis across multiple tissues

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