A Novel Ratiometric Fluorescent Probe for Mercury (II) ions and Application in Bio-imaging

Abstract

Since the accumulation of mercury (II) ions in the environment and ecosystem causes serious problems to environment and disease, the recognition of Hg2+ ions and its bio-imaging is of high importance. In sight of the advantages of fluorescence probes, a new probe (PMH) was facilely synthesized by incorporating phenylimidazole fluorophore and 3-methyl-2- benzothiazolinone hydrazone hydrochloride monohydrate. The PMH probe exhibited a ratiometric response for Hg2+ ions with fluorescence intensity increasing at 520 nm and decreasing at 445 nm simultaneously. The PMH probe interacted with Hg2+ ions in seconds with high optical stability and showed good selectivity over other metal ions. In addition, the probe has excellent biocompatibility and imaging performance in cells and zebrafish.

Aryl-Phenanthro[9,10-d]imidazole: A Versatile Scaffold for the Design of Optical-Based Sensors

“…Gao et al described the Schiff base sensor P11 obtained via condensation between P1 and 3-methyl-2-benzothiazolinonehydrazine ( 35 ) for Hg 2+ detection (Figure a) . Probe P11 exhibited intense fluorescence emission at 445 nm, which was decreased with a concomitant new band located around 520 nm in the presence of Hg 2+ .…”

“…(b) Confocal microscopy images of MCF-7 cells with P11 and Hg 2+ ions (λ exc = 405 nm) treated with P11 (1 μM) and Hg 2+ ions (0–15 μM) Green Chanel: fluorescent image of emission between 430 and 460 nm; Red Channel: fluorescent image of emission between 490 and 550 nm. Adapted with permission from ref . Copyright 2019 MDPI.…”

Development of a multi-responsive fluorescence switch system based on a pyrimidine functionalized tetrarylethylene compound

“…[30][31][32] To date, no specific recognition group existed for complexing with Cr 3+ , resulting in a dearth of literature regarding fluorescent probe detection of Cr 3+ . 33,34 Therefore, the development of a novel fluorescent probe capable of detecting Cr 3+ is still worth studying. Given the unique optical properties of TAE molecules, constructing multi-stimuli-response materials based on TAE is a tempting prospect, anticipated to achieve multiple signal responses to diverse external stimuli, including light, acid/base, and Cr 3+ at the single-molecule level.…”

A Naked-Eye and Fluorescent Dual-Channel Probe for Rapid Detection of Hg2+ and Its Multiple Applications

“…[17][18][19] Several fluorescent probes have been reported to detect Hg 2 ＋ , among which probes designed based on specific reactions superior to other sensing mechanisms in terms of selectivity and sensitivity, most of these probes have achieved good results in emission wavelength, water solubility and bioimaging, but further exploration is needed in reaction speed and detection of actual samples. [20][21][22][23][24][25] Therefore, it is necessary to prepare a fluorescent probe with high sensitivity that can detect lower levels of Hg 2 ＋ in actual samples and organisms. In addition, the thioacetals deprotection mechanism is considered to be a favorable option.…”