A novel recombinant vaccine protecting mice against abrin intoxication

Wang, Junhong; Gao, Shan; Xin, Wenwen; Kang, Lin; Xu, Na; Zhang, Tao; Liu, Wensen; Wang, Jinglin

doi:10.1080/21645515.2015.1008879

Cited by 8 publications

(3 citation statements)

References 25 publications

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“…Abrin is a highly toxic protein with a relative molecular mass of 65 kDa 34 that is composed of an A-chain and a B-chain. The estimated lethal oral dose of abrin in humans is 0.1–1 μg/kg 35 , 36 . Unintentional abrin ingestion can quickly cause irreparable organ failure and even death, so rapid, label-free, and sensitive detection of abrin is desired.…”

Section: Resultsmentioning

confidence: 99%

A monolithically integrated microcantilever biosensor based on partially depleted SOI CMOS technology

et al. 2023

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This paper presents a monolithically integrated aptasensor composed of a piezoresistive microcantilever array and an on-chip signal processing circuit. Twelve microcantilevers, each of them embedded with a piezoresistor, form three sensors in a Wheatstone bridge configuration. The on-chip signal processing circuit consists of a multiplexer, a chopper instrumentation amplifier, a low-pass filter, a sigma-delta analog-to-digital converter, and a serial peripheral interface. Both the microcantilever array and the on-chip signal processing circuit were fabricated on the single-crystalline silicon device layer of a silicon-on-insulator (SOI) wafer with partially depleted (PD) CMOS technology followed by three micromachining processes. The integrated microcantilever sensor makes full use of the high gauge factor of single-crystalline silicon to achieve low parasitic, latch-up, and leakage current in the PD-SOI CMOS. A measured deflection sensitivity of 0.98 × 10−6 nm−1 and an output voltage fluctuation of less than 1 μV were obtained for the integrated microcantilever. A maximum gain of 134.97 and an input offset current of only 0.623 nA were acquired for the on-chip signal processing circuit. By functionalizing the measurement microcantilevers with a biotin-avidin system method, human IgG, abrin, and staphylococcus enterotoxin B (SEB) were detected at a limit of detection (LOD) of 48 pg/mL. Moreover, multichannel detection of the three integrated microcantilever aptasensors was also verified by detecting SEB. All these experimental results indicate that the design and process of monolithically integrated microcantilevers can meet the requirements of high-sensitivity detection of biomolecules.

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Section: Resultsmentioning

confidence: 99%

A monolithically integrated microcantilever biosensor based on partially depleted SOI CMOS technology

et al. 2023

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“…Future protection efforts are aimed at developing a vaccine against inhaled ricin and abrin. No approved vaccine or specific antidote is currently available for human use, but experimental, recombinant vaccines are under development (Pittman et al 2015;Wang et al 2015). If exposure to skin occurs, decontamination with soap and water is available.…”

Section: Protectionmentioning

confidence: 99%

Highly Toxic Ribosome-Inactivating Proteins As chemical Warfare or Terrorist Agents

Patočka¹

2018

MMSL

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Biological weapons include infectious agents and toxins. Toxins are poisons produced by living organisms. An important group of toxins are ribosome inactivating proteins (RIPs) of plant or microbial origin that inhibit protein synthesis by inactivating ribosomes. RIPs have been of great scientific interest due to their importance in human health, as both pathogenic agents and therapeutics, but also due to their potential use in biological warfare and bioterrorism. RIPs relevant to bioterrorism include mainly ricin and abrin. Ricin is protein produced in the seeds of the castor oil plant (Ricinus communis). Abrin is protein that has been isolated from the seeds of Abrus precatorius. Both inactivate ribosomes, which results in toxicity because of the inhibition of protein synthesis. Abrin and ricin are substances very toxic to humans in all types of administration, with the exception of oral administration. Symptoms include nausea, diarrhea, tachycardia, hypotension, and seizures. Treatment is supportive, and no antidote exists.

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“…In mice, generation of protective antibodies can be induced with a sub-lethal dose of abrin (10,11). Recombinant abrin A chain (12)(13)(14)(15) or the weaker abrin B chain (16)(17)(18)(19), can also induce anti-abrin antibody production. Up to now, only two neutralizing antibodies against Abrin poisoning have been reported, D6F10 and A7C4, but neither has entered clinical trials or been used directly in humans.…”

Section: Introductionmentioning

confidence: 99%

A Novel Humanized Anti-Abrin A Chain Antibody Inhibits Abrin Toxicity In Vitro and In Vivo

Peng

Shi

et al. 2022

Front. Immunol.

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Abrin, a type-II ribosome inactivating protein from the seed of Abrus precatorius, is classified as a Category B bioterrorism warfare agent. Due to its high toxicity, ingestion by animals or humans will lead to death from multiple organ failure. Currently, no effective agents have been reported to treat abrin poisoning. In this study, a novel anti-abrin neutralizing antibody (S008) was humanized using computer-aided design, which possessed lower immunogenicity. Similar to the parent antibody, a mouse anti-abrin monoclonal antibody, S008 possessed high affinity and showed a protective effect against abrin both in vitro and in vivo, and protected mice that S008 was administered 6 hours after abrin. S008 was found that it did not inhibit entry of abrin into cells, suggesting an intracellular blockade capacity against the toxin. In conclusion, this work demonstrates that S008 is a high affinity anti-abrin antibody with both a neutralizing and protective effect and may be an excellent candidate for clinical treatment of abrin poisoning.

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A novel recombinant vaccine protecting mice against abrin intoxication

Cited by 8 publications

References 25 publications

A monolithically integrated microcantilever biosensor based on partially depleted SOI CMOS technology

A monolithically integrated microcantilever biosensor based on partially depleted SOI CMOS technology

Highly Toxic Ribosome-Inactivating Proteins As chemical Warfare or Terrorist Agents

A Novel Humanized Anti-Abrin A Chain Antibody Inhibits Abrin Toxicity In Vitro and In Vivo

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