A novel role of hippocalcin in bFGF‐induced neurite outgrowth of H19‐7 cells

Oh, Doo-Yi; Cho, Ju Hwan; Park, Shin-Young; Kim, Yong Seok; Yoon, Young-Ju; Yoon, Shin Hee; Chung, Kwang Chul; Lee, Ki Sung; Han, Joong‐Soo

doi:10.1002/jnr.21602

Cited by 15 publications

(22 citation statements)

References 35 publications

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“…Repressing Hes1 expression promotes neurite outgrowth, while overexpression of the Hes1 gene has been shown to have the opposite effect4041. The effects of Ngn1 and NeuroD on neurite outgrowth have also been extensively studied, and the two genes are critical to the promotion of neurite outgrowth4243444546. These results revealed that the potential mechanism by which RF-EMF exposure influences neurite outgrowth is the disruption of bHLH expression.…”

Section: Discussionmentioning

confidence: 97%

Exposure to 1800 MHz radiofrequency radiation impairs neurite outgrowth of embryonic neural stem cells

Chen

Liu

et al. 2014

Sci Rep

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A radiofrequency electromagnetic field (RF-EMF) of 1800 MHz is widely used in mobile communications. However, the effects of RF-EMFs on cell biology are unclear. Embryonic neural stem cells (eNSCs) play a critical role in brain development. Thus, detecting the effects of RF-EMF on eNSCs is important for exploring the effects of RF-EMF on brain development. Here, we exposed eNSCs to 1800 MHz RF-EMF at specific absorption rate (SAR) values of 1, 2, and 4 W/kg for 1, 2, and 3 days. We found that 1800 MHz RF-EMF exposure did not influence eNSC apoptosis, proliferation, cell cycle or the mRNA expressions of related genes. RF-EMF exposure also did not alter the ratio of eNSC differentiated neurons and astrocytes. However, neurite outgrowth of eNSC differentiated neurons was inhibited after 4 W/kg RF-EMF exposure for 3 days. Additionally, the mRNA and protein expression of the proneural genes Ngn1 and NeuroD, which are crucial for neurite outgrowth, were decreased after RF-EMF exposure. The expression of their inhibitor Hes1 was upregulated by RF-EMF exposure. These results together suggested that 1800 MHz RF-EMF exposure impairs neurite outgrowth of eNSCs. More attention should be given to the potential adverse effects of RF-EMF exposure on brain development.

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Section: Discussionmentioning

confidence: 97%

Exposure to 1800 MHz radiofrequency radiation impairs neurite outgrowth of embryonic neural stem cells

Chen

Liu

et al. 2014

Sci Rep

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“…PLD activity was determined by the formation of phosphatidylbutanol (PBt), the product of PLD-mediated transphosphatidylation in the presence of butanol as described previously, with a slight modification [27]. The cells, which had been incubated for the indicated times in 6-well plates, were radioactively labeled with 2 µCi/mL of [ 3 H]-palmitic acid in serum-free medium for 20 h, then pretreated with 0.3% (v/v) 1-butanol for 15 min before stimulation with leptin.…”

Section: Methodsmentioning

confidence: 99%

Leptin Increases TNF-α Expression and Production through Phospholipase D1 in Raw 264.7 Cells

Lee

Choi

et al. 2014

PLoS ONE

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Epidemiological evidence suggests that obesity is associated with inflammation of the respiratory tract and the pathogenesis of asthma. The purpose of this study was to examine the role of phospholipase D1 (PLD1) in leptin-induced expression of the proinflammatory cytokine, tumor necrosis factor (TNF)-α, and to suggest a molecular link between obesity and respiratory tract inflammation. We investigated whether leptin, a typical adipocytokine, plays a role in the expression of TNF-α through increased PLD1 activity in Raw 264.7. Leptin enhanced the activity of PLD1 through activation of PLCγ and Src, while PLD1 siRNA decreased the leptin-induced expression and production of TNF-α. Leptin-induced PLD activation was also inhibited by a PLCγ inhibitor (PAO) and Src kinase inhibitor (PP2), indicating that PLCγ and Src kinase are upstream activators of PLD1. Down-regulation of PLD1 also completely blocked activation of p70S6K, an activator of JNK. Leptin-induced expression of TNF-α was also prevented by inhibition of p70S6K and JNK. Taken together, these results indicate that PLD1 acts as an important regulator of leptin-induced expression of TNF-α by participating in the PLCγ/Src/PLD1/PA/p70S6K/JNK pathway.

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“…It also regulates mixed-lineage kinase 2, phospholipase D (PLD), and neuronal apoptosis inhibitory protein (Burgoyne and Weiss, 2001, Kobayashi et al., 1993, Lindholm et al., 2002, Nagata et al., 1998, O'Callaghan et al., 2002, O'Callaghan et al., 2003). We have reported that HPCA is a major regulatory protein in the Ca 2+ -mediated PLD signaling pathway (Oh et al., 2006) and that it induces expression of Neuro-D, leading to neurite outgrowth in H19-7 cells during differentiation (Oh et al., 2008). It is possible that HPCA regulates neurogenesis through these molecules; however, molecular mechanisms by which HPCA affects neuronal/astrocyte cell-fate decision have not been studied.…”

Section: Introductionmentioning

confidence: 99%

Hippocalcin Promotes Neuronal Differentiation and Inhibits Astrocytic Differentiation in Neural Stem Cells

et al. 2017

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SummaryHippocalcin (HPCA) is a calcium-binding protein that is restricted to nervous tissue and contributes to neuronal activity. Here we report that, in addition to inducing neurogenesis, HPCA inhibits astrocytic differentiation of neural stem cells. It promotes neurogenesis by regulating protein kinase Cα (PKCα) activation by translocating to the membrane and binding to phosphoinositide-dependent protein kinase 1 (PDK1), which induces PKCα phosphorylation. We also found that phospholipase D1 (PLD1) is implicated in the HPCA-mediated neurogenesis pathway; this enzyme promotes dephosphorylation of signal transducer and activator of transcription 3 (STAT3[Y705]), which is necessary for astrocytic differentiation. Moreover, we found that the SH2-domain-containing tyrosine phosphatase 1 (SHP-1) acts upstream of STAT3. Importantly, this SHP-1-dependent STAT3-inhibitory mechanism is closely involved in neurogenesis and suppression of gliogenesis by HPCA. Taken together, these observations suggest that HPCA promotes neuronal differentiation through activation of the PKCα/PLD1 cascade followed by activation of SHP-1, which dephosphorylates STAT3(Y705), leading to inhibition of astrocytic differentiation.

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A novel role of hippocalcin in bFGF‐induced neurite outgrowth of H19‐7 cells

Cited by 15 publications

References 35 publications

Exposure to 1800 MHz radiofrequency radiation impairs neurite outgrowth of embryonic neural stem cells

Exposure to 1800 MHz radiofrequency radiation impairs neurite outgrowth of embryonic neural stem cells

Leptin Increases TNF-α Expression and Production through Phospholipase D1 in Raw 264.7 Cells

Hippocalcin Promotes Neuronal Differentiation and Inhibits Astrocytic Differentiation in Neural Stem Cells

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