A novel sandwich-type photoelectrochemical immunosensor based on Ru(bpy)32+ and Ce-CdS co-sensitized hierarchical ZnO matrix and dual-inhibited polystyrene@CuS-Ab2 composites

Fan, Dawei; Liu, Xin; Bao, Chunzhu; Feng, Jinhui; Wang, Huan; Ma, Hongmin; Wu, Dan; Wei, Qin

doi:10.1016/j.bios.2019.01.029

Cited by 35 publications

(15 citation statements)

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“…We coupled the AmpC enzyme and PCT detection antibody to the surface of PS microspheres simultaneously to form a PS-Ab 2 /(AmpC) n complex as another signal amplification system (as shown in Scheme ). A large number of carboxyl binding sites on the surface of PS microspheres led to high efficiency for the conjugation of enzyme and antibody proteins . The effect of signal amplification can be adjusted by the mole ratios of the enzyme to the antibody proteins.…”

Section: Resultsmentioning

confidence: 99%

Fluorescence Resonance Energy Transfer-Mediated Immunosensor Based on Design and Synthesis of the Substrate of Amp Cephalosporinase for Biosensing

Deng

Zhang

et al. 2019

Anal. Chem.

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The traditional enzyme-linked immunosorbent assay (ELISA) has some disadvantages, such as insufficient sensitivity and low stability of the labeled enzyme, which limit its further applications. In this study, a more stable enzyme, Amp cephalosporinase (AmpC), was selected as the labeled enzyme, and its substrate was designed and synthesized. This substrate contained the cephalosporin ring core as the enzymatic recognition section and the structural motif of the 3-hydroxyflavone (3-HF) as the reporter molecule. AmpC can specifically catalyze the substrate and release 3-HF, which can enter the cavity of β-cyclodextrin (β-CD) on the surface of ZnS quantum dots and form a fluorescence resonance energy transfer (FRET) signal amplification system. An AmpCcatalyzed, FRET-mediated ultrasensitive immunosensor (ACF immunosensor) for procalcitonin (PCT) was developed by combining the signal amplification system of the polystyrene microspheres and effective immune-based magnetic separation. The ACF immunosensor has high sensitivity and specificity for the detection of PCT: its linear range is from 0.1 ng mL −1 to 70 ng mL −1 , and the limit of detection can reach 0.03 ng mL −1 . The spiking recoveries of PCT in human serum samples range from 98.3% to 107%, with relative standard deviations ranging from 2.14% to 12.0%. This approach was applied to detect PCT in real patient serum samples, and the results are consistent with those obtained with a commercial ELISA kit.

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Section: Resultsmentioning

confidence: 99%

Fluorescence Resonance Energy Transfer-Mediated Immunosensor Based on Design and Synthesis of the Substrate of Amp Cephalosporinase for Biosensing

Deng

Zhang

et al. 2019

Anal. Chem.

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“…Owing to the high stability, low cost, and convenient labeling, nanoparticles can also serve as signal probes for DNA detection to further improve the performance of sandwich assays [106][107][108][109][110][111][112][113][114][115]. For example, Zhang et al [116] reported a nanoparticle based "sandwich" detection strategy by immobilizing gold nanoparticles (AuNPs) with reporter probes that flank the target nucleic acids.…”

Section: +mentioning

confidence: 99%

Nucleic Acids Analysis

et al. 2020

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In recent years, multi-modal entity linking (MEL) has garnered increasing attention in the research community due to its significance in numerous multi-modal applications. Video, as a popular means of information transmission, has become prevalent in people's daily lives. However, most existing MEL methods primarily focus on linking textual and visual mentions or offline videos's mentions to entities in multi-modal knowledge bases, with limited efforts devoted to linking mentions within online video content. In this paper, we propose a task called Online Video Entity Linking (OVEL), aiming to establish connections between mentions in online videos and a knowledge base with high accuracy and timeliness. To facilitate the research works of OVEL, we specifically concentrate on live delivery scenarios and construct a live delivery entity linking dataset called LIVE. Besides, we propose an evaluation metric that considers timelessness, robustness, and accuracy. Furthermore, to effectively handle OVEL task, we leverage a memory block managed by a Large Language Model and retrieve entity candidates from the knowledge base to augment LLM performance on memory management. The experimental results prove the effectiveness and efficiency of our method.

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“…Reduced graphene oxide (rGO), one of chemically derived graphenes, possesses high electrical conductivity (as compared with graphene oxide (GO)), a high specific surface area, excellent biocompatibility, and abundant chemically active sites for chemical functionalization and catalysis. − Because of its excellent chemical and physical properties, rGO has been widely exploited in flexible electronics, batteries, supercapacitors, photodetectors, sensors, and so on. − However, rGO has a tendency to agglomerate or restack through π–π stacking and van der Waals interactions. , Chemical modifications or stabilizers were usually used to improve the dispersibility of rGO, − but these methods often reduced the analytical performance of graphene. Recently, various methods have emerged to overcome the issues of rGO dispersion, and one of the promising strategies involves the assembly of graphene onto a colloidal substrate, forming colloidal composite particles. − Polystyrene (PS) spheres are a commonly used colloidal substrate for composite material synthesis due to their size uniformity, resistance to acid–base corrosion, and established surface chemistries . Very little work, however, has been reported for utilizing graphene in a composite-particle configuration, , and current methods so far have only used microsized spheres.…”

Section: Introductionmentioning

confidence: 99%

Platinum Nanoparticle-decorated Graphene Oxide@Polystyrene Nanospheres for Label-free Electrochemical Immunosensing of Tumor Markers

Lan

Ren

Lambert

et al. 2020

ACS Sustainable Chem. Eng.

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We reported the synthesis of novel platinum-nanoparticle-decorated reduced graphene oxide@polystyrene nanospheres (PtNPs@rGO@PS NSs) and their use in developing a novel label-free electrochemical immunosensor for tumor markers. The PtNPs@rGO@PS NSs were fabricated via the electrostatic self-assembly of negatively charged graphene oxide nanosheets onto positively charged PS nanospheres, followed by hydrazine reduction of the GO outer layer and the in situ deposition of PtNPs. The PtNPs@rGO@PS NSs were characterized by scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy. The PtNPs@rGO@PS NSs show a large specific surface area, high electrical conductivity, and good hydrophilicity. The nanospheres were further biofunctionalized with streptavidin and a biotinylated antibody for use as a highly sensitive electrochemical immunosensor for the label-free determination of tumor marker carcinoembryonic antigen (CEA). The fabricated immunosensor detected concentrations of CEA ranging from 0.05 to 70 ng/mL with a detection limit of 0.01 ng/mL, presenting high sensitivity comparable to standard commercial techniques. The nanospheres also showed excellent specificity against other tumor markers and reliability in detection with clinical samples.

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A novel sandwich-type photoelectrochemical immunosensor based on Ru(bpy)32+ and Ce-CdS co-sensitized hierarchical ZnO matrix and dual-inhibited polystyrene@CuS-Ab2 composites

Cited by 35 publications

References 50 publications

Fluorescence Resonance Energy Transfer-Mediated Immunosensor Based on Design and Synthesis of the Substrate of Amp Cephalosporinase for Biosensing

Fluorescence Resonance Energy Transfer-Mediated Immunosensor Based on Design and Synthesis of the Substrate of Amp Cephalosporinase for Biosensing

Nucleic Acids Analysis

Platinum Nanoparticle-decorated Graphene Oxide@Polystyrene Nanospheres for Label-free Electrochemical Immunosensing of Tumor Markers

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