A novel sandwiched electrochemiluminescence immunosensor for the detection of carcinoembryonic antigen based on carbon quantum dots and signal amplification

Li, Nian-Lu; Jia, Liping; Ma, Rong-Na; Jia, Wen-Li; Lu, Yijun; Shi, Sha-Shan; Wang, Huaisheng

doi:10.1016/j.bios.2016.04.020

Cited by 109 publications

(34 citation statements)

References 50 publications

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“…Finally, the excited CQD * returns to the ground state via a radical pathway emitting a photon. This property is mainly used for sensing as exemplified by the electrochemiluminescent immunosensor developed by Li et al 73 Tuning the emission spectrum from the green to the near infrared has been achieved by Sun et al using surface passivation. 74 Doping fluorescent CQDs with heteroatoms is another feasible way to tune the intrinsic properties of CQDs and GQDs.…”

Section: Optical Propertiesmentioning

confidence: 99%

Carbon-based quantum particles: an electroanalytical and biomedical perspective

et al. 2019

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Section: Optical Propertiesmentioning

confidence: 99%

Carbon-based quantum particles: an electroanalytical and biomedical perspective

et al. 2019

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“…Additionally, labeling different targets with multicolor QDs enables multiplex ECL assays [53]. Also, graphene quantum dot (GQD)/carbon dots (CDs) is an ideal nanomaterial with ECL performance due to its unique optical properties of high photostability, high electrical conductivity and tunable excitation, as well as a large surface area to immobilize biomolecules [54,55,56,57]. Especially, N heteroatoms doping GQDs (N-GQDs) can dramatically alter the electro-optical properties of GQDs.…”

Section: Electrochemiluminescence (Ecl)-based Detection Of Pathogenmentioning

confidence: 99%

Recent Advances in Electrochemiluminescence Sensors for Pathogenic Bacteria Detection

2019

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Pathogenic bacterial contamination greatly threats human health and safety. Rapidly biosensing pathogens in the early stage of infection would be helpful to choose the correct drug treatment, prevent transmission of pathogens, as well as decrease mortality and economic losses. Traditional techniques, such as polymerase chain reaction and enzyme-linked immunosorbent assay, are accurate and effective, but are greatly limited because they are complex and time-consuming. Electrochemiluminescence (ECL) biosensors combine the advantages of both electrochemical and photoluminescence analysis and are suitable for high sensitivity and simple pathogenic bacteria detection. In this review, we summarize recent advances in ECL sensors for pathogenic bacteria detection and highlight the development of paper-based ECL platforms in point of care diagnosis of pathogens.

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“…Carcinoembryonic antigen (CEA), a highly glycosylated protein with molecular weight of approximately 200 kDa, is one of the most widely used tumor markers for diagnosis, prognosis estimation and monitoring of malignant tumors such as pancreatic, colorectal, liver, breast and gastric cancers 1,2 . The level of CEA in the healthy human blood is in minimal level, while an elevated concentration of >5 ng mL −1 can be detected when cancer cells are formed.…”

Section: Introductionmentioning

confidence: 99%

Graphene Quantum Dots Decorated Gold-Polyaniline Nanowire for Impedimetric Detection of Carcinoembryonic Antigen

Ganganboina

Doong

2019

Sci Rep

105

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A label-free impedimetric immunosensor based on N, S-graphene quantum dots@Au-polyaniline (N, S-GQDs@Au-PANI) nanowires was fabricated for the quantitative detection of carcinoembryonic antigen (CEA). The N, S-GQDs and Au-PANI were synthesized by a simple hydrothermal pyrolysis and interfacial polymerization, respectively. Subsequently, 2–9 nm N, S-GQDs are successfully decorated onto 30–50 nm Au-PANI nanowires by Au-thiol linkage to serve as the bifunctional probe for amplifying the electrochemical activity as well as anchoring anti-CEA. The N, S-GQDs@Au-PANI nanowires are excellent conducting materials to accelerate the electron transfer, while the formation of CEA antibody-antigen bioconjugates after the addition of CEA significantly increase the charge transfer resistance, and subsequently provides a highly stable and label-free immunoassay platform for the impedimetric detection of CEA. The label-free immunosensor exhibits a wide linear range from 0.5 to 1000 ng mL −1 with a low detection limit of 0.01 ng mL −1 . The N, S-GQDs@Au-PANI based immunosensor also shows high selectivity and stability over other cancer makers and amino acids. Moreover, this promising platform is successfully applied to the detection of CEA in human serum samples with excellent recovery of (96.0 ± 2.6)–(103 ± 3.8)%. These results clearly demonstrate a newly developed highly efficient and label-free impedimetric immunosensor for the detection of CEA using N, S-GQDs@Au-PANI nanowires as the biosensing probe, which can pave the gateway for the fabrication of high performance and robust impedimetric immunosensor to detect cancer makers in early stage of cancer diagnosis and therapy.

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A novel sandwiched electrochemiluminescence immunosensor for the detection of carcinoembryonic antigen based on carbon quantum dots and signal amplification

Cited by 109 publications

References 50 publications

Carbon-based quantum particles: an electroanalytical and biomedical perspective

Carbon-based quantum particles: an electroanalytical and biomedical perspective

Recent Advances in Electrochemiluminescence Sensors for Pathogenic Bacteria Detection

Graphene Quantum Dots Decorated Gold-Polyaniline Nanowire for Impedimetric Detection of Carcinoembryonic Antigen

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