A novel sequence-based coa genotyping method to discriminate nosocomial methicillin-resistant Staphylococcus aureus isolates

Li, Q. T.; Zhu, Yongzhang; Dong, Ke; Liu, C.; Zhou, Yuhua; Ni, Yizhao; Guo, Xia

doi:10.1007/s11845-011-0680-x

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Mitain et al, also detect 6 different patterns for coa gene in 35 MRSA isolates in Japan based on PCR-RFLP method (23). Li Q et al, study also showed S11 type of C1 and C2 region of coa gene in hospital isolates (24). In the current study, 10 different patterns of spa gene were determined by PCR, which were similar to those of Harmsen et al study in Germany, they could determine 10 different patterns of spa gene in 191 MRSA isolates of a university hospital by spa typing (25).…”

Section: Discussionsupporting

confidence: 80%

Molecular Investigation of Staphylococcus aureus, coa and spa Genes in Ahvaz Hospitals, Staff Nose Compared With Patients Clinical Samples

Afrough

Pourmand

Sarajian³

et al. 2013

Jundishapur J Microbiol

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Background: Staphylococcus aureus is one of the important human pathogens which are mainly isolated from wound, skin and contaminated respiratory excretions. Because many of hospital staff and patients carry this pathogen in their nose or skin, close contacts and touching have special role in spreading the infection in hospitals. Also, antibiotic resistant S. aureus, especially Methicillin Resistant S. Aureus (MRSA) have been seen among subjects. Thus, there should be an investigation for Bacteria colonization in nose of hospital staff and patients. Furthermore, investigation of antibiotic resistance pattern and examination of genotyping properties of resistant strains have a high efficacy in control and recognition of infection origin.Objectives: The current study aimed to determine the characteristics of S. aureus isolated from patients and staff in hospitals and compare them based on coa and spa typing methods.Materials and Methods: In the current study, 157 clinical specimens were collected from patients who were treated at the Ahvaz medical university hospitals including 79 specimens (50.3%) from Sina hospital, 34 specimens (21.7%) from Imam Khomeini hospital, and 44 specimens (28%) from Golestan hospital and 157 nose swab specimens from the staff of these hospitals were collected during 2010. coa, spa genes of isolated Bacteria were amplified using PCR.Results: PCR results showed seven different patterns for staff and five different patterns for patients based on spa gene, and for coa gene five and six different patterns respectively. In addition, the prevalence of MRSA was 52.5 in staff and 83.7 in patients' specimens. Comparison of genetic diversity of spa, and coa genes in Ahvaz university hospitals doesn’t show significant difference (Chi-square and fisher's exact test).Conclusions: The outcome of this study show that spa and coa typing are suitable methods for MRSA isolates typing because it is easy to use and interpret them, and that these methods can be useful in infection source detection and its control especially in epidemic situation

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Section: Discussionsupporting

confidence: 80%

Molecular Investigation of Staphylococcus aureus, coa and spa Genes in Ahvaz Hospitals, Staff Nose Compared With Patients Clinical Samples

Afrough

Pourmand

Sarajian³

et al. 2013

Jundishapur J Microbiol

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“…5 Many molecular techniques are utilized to group isolates of S. aureus: Multi-Locus Sequence Typing (MLST), antibiotic sensitivity testing, Pulsed Field Gel Electrophoresis (PFGE) and PCR based assays: SCCmec typing, spa typing, coa typing, agr typing, phage typing, Automated Repetitive PCR, Panton-Valentine leukotoxin (pvl) PCR. [6][7][8][9] With the exception of direct PCR for virulence genes, these methods provide a general characterization of the isolate(s) based on previously collected empirical data. This protocol provides a direct method to identify isolates that have an a-toxin hyper-producing phenotype and are likely to cause severe bovine mastitis, allowing treatment to be initiated quickly and reducing the spread of the infection.…”

Section: Introductionmentioning

confidence: 99%

Identification of predominant SNPs as a novel method for genotyping bovineStaphylococcus aureusisolates

Hall

Ji²

2012

Virulence

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Characterization of Staphylococcus aureus faecal isolates associated with food-borne disease in Korea

et al. 2016

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A novel sequence-based coa genotyping method to discriminate nosocomial methicillin-resistant Staphylococcus aureus isolates

Abstract: It was demonstrated that this coa method is a simple, rapid, and effective technique applicable for outbreak or local nosocomial MRSA investigations in the future.

Cited by 3 publications

References 22 publications

Molecular Investigation of Staphylococcus aureus, coa and spa Genes in Ahvaz Hospitals, Staff Nose Compared With Patients Clinical Samples

Molecular Investigation of Staphylococcus aureus, coa and spa Genes in Ahvaz Hospitals, Staff Nose Compared With Patients Clinical Samples

Identification of predominant SNPs as a novel method for genotyping bovineStaphylococcus aureusisolates

Characterization of Staphylococcus aureus faecal isolates associated with food-borne disease in Korea

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