A novel SERS biosensor for ultrasensitive detection of HPV-E7 and OPN based on a cascade signal amplification strategy of catalytic hairpin assembly and hybridization chain reaction

Lü, Dan; Li, Zhiyue; Gu, Yingyan; Ge, Shengjie; Mao, Yu; Gu, Yuexing; Cao, Xiaowei

doi:10.1039/d2qm00217e

Cited by 16 publications

(5 citation statements)

References 38 publications

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“…Due to its unique amplification products, it is easy to generate excessive byproducts, resulting in excessively high background signals ( Bi et al., 2017 ). ECL methods have a low background, portable instruments, and easy operation, which significantly increase sensitivity ( Dan et al., 2022 ; Lu et al., 2022 ; Wu et al., 2022 ). However, the nucleic acid amplification process and detection process are still divided into two steps.…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

“…This disassembly of hairpin DNA structures enables 100-fold H1/H2 double-stranded amplification in a short period of time. Dan Lu et al used and HCR (Dan et al, 2022) in conjunction with a surface-enhanced Raman scattering (SERS) biosensor to design a method to detect HR-HPV. This method provides a signal enhancement of 6-14 orders of magnitude compared to that of conventional Raman signals (Figure 4D) and uses HR-HPV conjugates as triggers to self-assemble hairpin DNA into CHA products.…”

Section: Cha Assaysmentioning

confidence: 99%

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Portable, and ultrasensitive HR-HPV tests based on nucleic acid biosensors

Ma,

Zou,

et al. 2024

Front. Cell. Infect. Microbiol.

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Cervical cancer is the third most common cancer threatening women’s health globally, and high-risk human papillomavirus (HR-HPV) infection is the main cause of cervical cancer worldwide. Given the recurrent nature of HR-HPV infection, accurate screening is essential for its control. Since the commonly used polymerase chain reaction (PCR) technique is limited by professional equipment and personnel, convenient and ultrasensitive detection methods for HR-HPV are still highly needed. As new molecular detection methods, nucleic acid amplification-based biosensors have the advantages of high sensitivity, rapid operation, and portability, which are helpful for point-of-care testing in rural and remote areas. This review summarized nucleic acid biosensors for HR-HPV screening based on a variety of nucleic acid amplification strategies involved in improved PCR, loop-mediated isothermal amplification, recombinase polymerase amplification, hybridization chain reaction, catalyzed hairpin assembly, and CRISPR/Cas systems. In combination with microfluidic technology, lateral flow assays, electrochemical analysis and other sensing technologies, HR-HPV nucleic acid biosensors have the advantages of high throughput, short response time, high sensitivity and easy operation in the field. Although there are still shortcomings, such as high cost and poor reproducibility, this approach will be suitable for on-site screening of HR-HPV infection or cervical cancer and for auxiliary clinical diagnosis in complex environments and poor areas in the future.

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Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Section: Cha Assaysmentioning

confidence: 99%

Portable, and ultrasensitive HR-HPV tests based on nucleic acid biosensors

Ma,

Zou,

et al. 2024

Front. Cell. Infect. Microbiol.

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“…Recently, a surface-enhanced Raman spectroscopy (SERS) sensor was reported by Lu et al as an assay for HPV-E7 and OPN [215] (Figure 5). The sensor provided an ultralow LOD of 0.76 pg/mL and 0.62 pg/mL for HPV-E7 and OPN, respectively, and a wide linear range of 1 pg/mL to 1 μg/mL in serum.…”

Section: Sensors and Diagnostics Accepted Manuscriptmentioning

confidence: 99%

“…Recently, a surface-enhanced Raman spectroscopy (SERS) sensor was reported by Lu et al as an assay for HPV-E7 and OPN 215 (Fig. 5).…”

Section: Sensors and Diagnostics Critical Reviewmentioning

confidence: 99%

Sensor detection in gynaecological medicine

et al. 2022

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“…To effectively improve electrochemical biosensing analysis and make it suitable for quantitative analysis of trace biomolecules, signal amplification strategies like nanoparticle amplification, − biological enzyme catalysis, − molecular biotechnology amplification, − and dendrimer method − are commonly used but still have some drawbacks. In recent years, the polymer-based signal amplification strategy has proved to be effective by labeling the polymer material with a large number of detection probes or active sites for subsequent modification.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition

Sun

Liu

Kong

et al. 2023

ACS Appl. Mater. Interfaces

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Quantitative measurement of microRNAs (miRNAs) is extremely important in plenty of biomedical applications especially cancer diagnosis but remains a great challenge. In this work, we developed a logic gate recognition biosensing platform based on the “trinity” molecular recognition mode for quantifying miRNAs with a detection limit of 4.48 aM, along with a linear range from 0.1 nM to 10 aM under optimal experimental conditions. In order to obtain excellent detection performance, we adopted a Zn(TCPP) photocatalytic electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization signal amplification strategy. The light-induced PET-RAFT has developed green applications of free radical polymerization in the field of biosensors. This is the first report on the preparation of signal amplification biosensors using PET-RAFT for tumor marker detection. With the outstanding detection performance, we can apply the sensor system to the early screening of lung cancer patients.

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A novel SERS biosensor for ultrasensitive detection of HPV-E7 and OPN based on a cascade signal amplification strategy of catalytic hairpin assembly and hybridization chain reaction

Abstract: Human papillomavirus E7 protein (HPV-E7) and osteopontin (OPN) have been established as critical biomarkers for the early diagnosis of cervical cancer. However, it is currently lacking in highly sensitive and...

Cited by 16 publications

References 38 publications

Portable, and ultrasensitive HR-HPV tests based on nucleic acid biosensors

Portable, and ultrasensitive HR-HPV tests based on nucleic acid biosensors

Sensor detection in gynaecological medicine

Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition

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