A novel soy protein isolate prepared from soy protein concentrate using jet-cooking combined with enzyme-assisted ultra-filtration

Prevention of retinoic acid‐induced osteoporosis in mice by isoflavone‐enriched soy protein

“…The SPI dispersions (2.0%, w/v) were prepared by dissolving the lyophilised SPI in distilled water and stirring at room temperature (25 °C) for 2 h. The heated SPC dispersions (HSPC, 2.0%, w/v) were prepared using a jet cooking (JC) system (Nan‐Liang Food Machine Company Co. Ltd, Guangzhou, China) at 130 °C for 90 s and JM‐S0522W 80 kDa hollow fibre membranes (Guangzhou Jason Membrane Technology Co., Ltd, Guangzhou, China) with SPC as a starting material . The Mix was obtained from appropriate amounts of SPI and IS dispersed in water and stirred gently at room temperature (25 °C) for 2 h. The Mix contained 20 mg mL −1 proteins. We prepared the SPA‐IS (pH 6.4) from the Mix, heated our preparation at 120 °C for 15 min in a water bath, and immediately cooled in an ice bath.…”

“…We prepared the SPA‐IS (pH 6.4) from the Mix, heated our preparation at 120 °C for 15 min in a water bath, and immediately cooled in an ice bath. The isoflavones were analysed by high‐performance liquid chromatography (HPLC) according to Yang et al , and the fluorescence spectra were recorded using an F7000 fluorescence spectrophotometer (Hitachi Co., Tokyo, Japan) as described by Wan et al The digestion procedure was a slight modification of the method described by Chavan et al and thus we added no pancreatic lipase.…”

“…Visceral index, which refers to the ratio of organ mass to body weight, indicates the influence of drugs on organs and yields a preliminary toxicity judgment. Thymus, liver, spleen and uterus were collected and weighed, from which we calculated a post‐mortem visceral index . We reserved uterus and the ovary for morphological observation of endometrial and ovarian tissues.…”

“…The serum samples were used to measure biochemical indicators at the serum level with a suitable kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and a Thermo Scientific Genesys 10S UV–visible spectrophotometer (Thermo Fisher Scientific Co., Madison, WI, USA), including serum calcium (s‐Ca), serum phosphorus (s‐P), serum alkaline phosphatase (s‐ALP), and serum tartrate‐resistant acid phosphatase (s‐TRACP). The serum samples were also applied to measure bone metabolism‐related hormone levels of serum osteocalcin (s‐BGP) and serum mouse oestradiol (s‐E 2 ) by an immunological method using a Victor3 Multilabel Counter 1420 (PerkinElmer, Waltham, MA, USA).…”

Recovery of high value‐added protein from enzyme‐assisted aqueous extraction (EAE) of soybeans by dead‐end ultrafiltration

“…In order to reduce the concentration polarization effect, stirring of the feed solution was conducted using a magnetic stirrer fixed over the membrane surface and rotating at a constant rate of 450 rpm. The permeate flux was monitored throughout the process by recording the volume of permeate with a graduated cylinder and calculated as (Yang et al, 2014):…”