A Novel Strategy for Quantitative Analysis of Major Ginsenosides in Panacis Japonici Rhizoma with a Standardized Reference Fraction

Abstract

Panacis Japonici Rhizoma (Zhu-Jie-Shen in Chinese), the root of P. japonicus C.A. Mey., is commonly used in traditional Chinese Medicine. Saponins are the major bioactive compounds in this herb. The similarity of polarity and structure of the natural products in herb caused the difficulty of purification and resulted in the shortage and high cost of the reference compounds, which has greatly hindered efforts toward quantification in quality control. A novel strategy using a standardized reference fraction for qualification of the major saponins in Panacis Japonici Rhizoma was proposed to easily and effectively control the quality of PJR. The strategy is feasible and reliable, and the methodology of the developed approach is also validated. The standardized reference fraction was used for quantification, which might solve the shortage of the pure reference compounds in the quality control of herbal medicines.

Techniques for extraction and isolation of natural products: a comprehensive review

“…8 ), in the obtained PJRS was more than 73%. The PJRS served as the standard reference for quality control of Panacis Japonici Rhizoma [ 56 ]. Some researchers assumed that the principal adsorptive mechanism between macroporous resins and polyphenols was associated with the hydrogen bonding formation between the oxygen atom of the ether bond of the resin and the hydrogen atom of phenolic hydroxyl group of the phenol.…”

Integration of Data-Dependent Acquisition (DDA) and Data-Independent High-Definition MSE (HDMSE) for the Comprehensive Profiling and Characterization of Multicomponents from Panax japonicus by UHPLC/IM-QTOF-MS

“…P. japonicus is characteristic for the high content of OA-saponins (such as ginsenoside Ro/chikusetsusaponin V, pseudoginsenoside Rt1, chikusetsusaponins IV, and IVa) [24,26]. In this work, we could characterize 76 OA-ginsenosides (42.7% of the total amount), of which 95 # (Ro), 122 # (p-Rt1), 127 # (IV), and 135 # (IVa) were identified with the aid of reference compounds.…”

“…P. japonicus C.A. Meyer (the rhizome) is used as a traditional herbal medicine, Panacis Japonici Rhizoma (Zhu-Jie-Shen), officially recorded in Chinese Pharmacopoeia (2015 edition), with the functions of dissipating blood stasis, detumescence, hemostasis, and reinforcing deficiency [23,24]. According to a review article of Reference [25], at least 111 saponins have been isolated from this species, which can be classified into the PPD, PPT, OT (octillol), OA, UA (ursolic acid), and DD II (dammarenediol II) subtypes.…”

Recent Advances in Separation and Analysis of Saponins in Natural Products

“…Ak and Am are the peak areas of the internal reference and other components to be tested, respectively, and Wk and Wm are the concentration (or mass) of the internal reference and other components to be tested, respectively [87]. This method is suitable for the simultaneous qualitative and quantitative determination of the same kind of multiple components when a reference substance is rare [122] and preparation cost is high [123].…”