A novel support of sponge-like cellulose composite polymer for immobilizing laccase and its application in nitrogenous organics biodegradation

Gu, Yaohua; Xue, Ping; Shi, Keren

doi:10.1007/s10934-019-00786-y

Cited by 15 publications

(15 citation statements)

References 44 publications

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“…The new trend of forward osmosis, aided by laccase, is used in the removal of micro-pollutants from wastewater and increase the potability of water [15]. Laccase is also used in the biodegradation of organics, as it is a critical factor in reducing water pollution with its excellent catalytic performance and reusability [16,17].…”

Section: Introductionmentioning

confidence: 99%

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Sayyed

Bhamare²,

Sapna

et al. 2020

PLoS ONE

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Although laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB_RZ4. The results obtained indicated that Aspergillus sp. HB_RZ4 produced a significantly large amount of laccase under meso-acidophilic shaking conditions in a medium containing glucose and yeast extract. A 25 μM CuSO 4 was observed to enhance the enzyme yield. The enzyme was best purified on a Sephadex G-100 column. The purified enzyme resembled laccase of A. flavus. The kinetics of the purified enzyme revealed high substrate specificity and good velocity of reaction,using ABTS as a substrate. The enzyme was observed to be stable over various pH values and temperatures. The peptide structure of the purified enzyme was found to resemble laccase of A. kawachii IFO 4308. The fungus was observed to decolorize various dyes independent of the requirement of a laccase mediator system. Aspergillus sp. HB_RZ4 was observed to be a potent natural producer of laccase, and it decolorized the dyes even in the absence of a laccase mediator system. Thus, it can be used for bioremediation of effluent that contains non-textile dyes.

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Section: Introductionmentioning

confidence: 99%

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Sayyed

Bhamare²,

Sapna

et al. 2020

PLoS ONE

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“…The medium that produced the most enzymes was then employed in optimization trials. The method described by Mahmoud et al (19) was used to optimize laccase production from endophytic fungus at various parameters; temperature https://www.indjst.org/ (25 to 45 o C with 10 o C increments), pH (4)(5)(6)(7)(8), number of discs (8mm in diameter) varied from 1 to 5, number of days (5)(6)(7)(8)(9)(10)(11), carbon source concentration range of 10 g/L (Glucose, Fructose, Galactose, Sucrose), nitrogenous source concentration range of 10 g/L (Ammonium Nitrate, Peptone, Sodium Nitrate, Urea). Two discs (8 mM in diameter) from a 5-day-old culture were injected into this sterilized media and incubated at pH-4.5 and 25 ±2 o C for 7 days.…”

Section: Optimization Studiesmentioning

confidence: 99%

“…With the help of laccase, the new method of forward osmosis is utilized to improve the potability of water and remove micropollutants from wastewater (8) . Due to its superior catalytic activity and reusability, laccase is also employed in the biodegradation of organics and is crucial in lowering water pollution (9,10) . https://www.indjst.org/ However, laccase's limited applicability due to its high production cost and ineffectiveness has reinforced the need to create an economically viable technique (11) .…”

Section: Introductionmentioning

confidence: 99%

Laccase Production Under Optimized Parameters by Aspergillus oryzae, an Endophytic Fungus and their Application to Waste Water Treatment

Naz¹,

Gupta²,

Chatterjee³

2023

IJST

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Objective: To isolate an endophytic fungal laccase producer from the Ziziphus mauritiana plant leaves in the surroundings of paper mill effluents and to study its role in the decolorization of synthetic dyes, removal of COD and phenol from industrial effluents. Methods: Laccase-producing endophytic fungi were isolated and screened by using an agar plate method. The positive isolates were identified, and laccase activity was determined by using spectrophotometric methods to monitor the oxidation of guaiacol and optimizing various parameters affecting laccase production. The molecular mass of the purified laccase enzyme was determined by using 12% SDSgel electrophoresis. Industrial effluents were treated with laccase to remove phenol, decolorize dyes, and reduce chemical oxygen demand. For the analysis, a spectrophotometric method was employed. Findings: One of the most effective endophytic fungal isolates, Aspergillus oryzae, was screened as a maximum laccase producer. The optimal pH of 6, temperature of 35 o C, inoculation period of 8 days, and the inoculum number of 3 discs/100 ml of Czapek Dox Broth in submerged culture were determined for the maximum laccase production. Sucrose and sodium nitrate, as carbon and nitrogen sources, considerably assisted laccase production. The molecular weight of the isolated laccase from A. oryzae was 66 kDa. The greatest activity was determined to be 64.2 U/mL, which is two times more than under unoptimized conditions. After the fifth day of exposure, the A. oryzae laccase decolorizes the synthetic dyes Bromophenol blue, Congo red, Methyl orange, and Phenol red.Chemical oxygen demand and phenolic pollutants' clearance rates were 38-43% and 60% from coal and textile effluents during their exposure times, respectively. Novelty: A. oryzae was discovered to be a potent natural laccase producer endophytic fungus from paper mill effluents, which may be used for decolorizing non-textile dyes, treatment of various industrial effluents, and other industrial purposes.

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“…Furthermore, pH 5 is already known as a condition for good results for laccase activity. 23 Suspension samples containing the enzymatic derivative were collected at each interval, following its oxidation activity through the methodology described in Section 2.5. The same procedure was performed for the laccase in its free form, in which laccase solution (0.5 mg/mL) was added to 0.1 mol/L of phosphate-citrate buffer with the same pH values as previously described.…”

Section: Stability Of Immobilized Laccase At Different Phsmentioning

confidence: 99%

Functionalized polyacrylonitrile particles as a promising support for the immobilization of laccase fromTrametes versicolor

Vieira

Henriques

Gurgel

et al. 2023

J of Applied Polymer Sci

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This work evaluates the potential of polyacrylonitrile particles (PAN) as support for laccase from Trametes versicolor immobilization. The slurry polymerization method forms mesoporous particles with low surface area and low maximum pore volume in desorption. The particles were chemically modified by consecutive alkaline and acid hydrolysis, followed by amination and activation with glutaraldehyde to enzyme immobilization. The laccase immobilization yield was 99.48% and 14.29% using the functionalized and non-functionalized particles, respectively. The enzyme activity was measured by the oxidation of 2,2 0 -azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) at different pHs and temperatures. The PAN/laccase derivative was hyperactivated at pH 3, up to 3 times higher than the free enzyme, and performed better at 50 C after 6 h of incubation, with relative activity up to 33% higher than the free enzyme. However, both enzymes denatured when the conditions reached pH 8 and 70 C. The PAN/laccase retained 89% of the initial activity after 30 days of storage at 5 C. It was possible to reuse the enzymatic derivative for 5 cycles, with up to 50% residual activity, under 50 C and pH 3. These results show the potential of this new support for laccase immobilization and further applications of industrial interest.

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A novel support of sponge-like cellulose composite polymer for immobilizing laccase and its application in nitrogenous organics biodegradation

Cited by 15 publications

References 44 publications

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Laccase Production Under Optimized Parameters by Aspergillus oryzae, an Endophytic Fungus and their Application to Waste Water Treatment

Functionalized polyacrylonitrile particles as a promising support for the immobilization of laccase fromTrametes versicolor

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