A novel system for rapid conversion of Gardner embryo grades to linear scale numeric variables

Pierson, Hannah; Invik, Jesse; Meriano, Jim; Pierson, R.A.

doi:10.1016/j.rbmo.2023.01.008

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…A 1 ml of follicular fluid was collected for measuring GDF-9 and BMP-15 using the same serum kit by ELISA. Microscopic assessment of oocytes maturity and quality was done by the embryologist according to the oocyte scoring system [21]. Luteal phase support using vaginal progesterone suppositories (Cyclogest 200 L.D.…”

Section: Methodsmentioning

confidence: 99%

Serum Follicular Fluid Growth Differentiation Factor 9 (GDF-9) and Bone Morphogenic Protein 15 (BMP-15) as Markers of Ovarian Reserve

Hassan,

Abdulhameed

2024

amj

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Background: Growth differentiation factor (GDF-9) and bone morphogenic protein (BMP-15) (GDF-9 co-factor) are proteins that are released by oocytes and control key stages of follicular development and growth. Objectives: To determine the ability of serum follicular fluid GDF-9 and BMP-15 in predicting ovarian reserve represented by their correlations with female age, traditional markers; antral follicle count (AFC) and anti-mullerian hormone (AMH). Materials and methods: In this prospective cohort study, 114 infertile females from the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Baghdad, Iraq, were included. Their age was 18-43 years old and their body mass index (BMI) was 19-30 Kg/m 2 . All were assessed on the second day of the menstrual cycle. Serum was taken to measure GDF-9 and BMP-15 using a special Kit by ELISA. Following oocyte retrieval, follicular fluid was collected for measuring GDF-9 and BMP-15. Results: The serum and follicular fluid GDF-9 and BMP-15 showed a significant correlation with the age of the patient, while there was no such association with AMH. Significant positive correlations were found between serum and follicular fluid GDF-9 and AFC, while only follicular fluid GDF-9 exhibited a significant positive correlation with mature II (MII) oocytes number. While, significant positive correlations were found between follicular fluid BMP-15 and the total number of oocytes. Conclusion: Serum follicular fluid GDF-9 and BMP-15 can be good predictors of ovarian reserve due to their significant negative correlations with age (oocyte quality) and significant positive correlations with AFC (quantity). Follicular fluid GDF-9, serum and follicular fluid BMP-15 can predict oocyte maturity.

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Section: Methodsmentioning

confidence: 99%

Serum Follicular Fluid Growth Differentiation Factor 9 (GDF-9) and Bone Morphogenic Protein 15 (BMP-15) as Markers of Ovarian Reserve

Hassan,

Abdulhameed

2024

amj

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“…The FISH investigation involved the use of probes for chromosomes 13, 18, 21, X, and Y. Subsequently, 25 donated embryos (euploid: 8 and aneuploid: 17) from ten couples at the blastocyst stage ( fth day) were selected based on the Gardner grading criteria, with a grade of A and at least B [12]. Trophectoderm cells were isolated from each blastocyst using a needle biopsy, and the samples were transferred to microtubes containing 100 µl Trizol.…”

Section: Methodsmentioning

confidence: 99%

Association of Trophectoderm mRNAs and MicroRNAs with Chromosomal Aneuploidy of Embryo

Esmaeilivand,

Ghasemzadeh,

Niknafs

et al. 2023

Reprod. Sci.

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MicroRNAs (miRNAs) and mRNAs can serve as indicators of the chromosomal state of an embryo, with different pro les observed in euploid and aneuploid blastocysts. Examining the levels of miRNAs associated with aneuploidy and euploidy, as well as mRNAs related to implantation, can aid in predicting blastocyst chromosomal normality and improving assisted reproductive technology (ART) outcomes.This study analyzed chromosomal abnromality of 25 blastocysts using uorescence in situ hybridization (FISH) and also the expression of genes ERBB4, SELL, ITGB3, and ITGAV, as well as miRNAs, miR-339, miR-27b, miR-661, miR-30c, miR-191, miR-345, miR-142, miR-141, miR-20a and, miR-372. We found that 17 out of 25 embryos were aneuploid. Moreover, results revealed lower expression levels of miR-30c and

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“…Subsequently, embryonic development assessments were conducted post-fertilization on days three and five. All 11 MII oocytes were fertilized, resulting in three cleavage-stage embryos and two blastocysts of grade 4AA and 3AA, rest got arrested into 2 pronuclear (2PN) stage, as per the Gardner grading system [ 16 ]. After the fresh embryo transfer (ET), the outcome was monitored by assessing β-human chorionic gonadotropin (β-hCG) levels.…”

Section: Case Presentationmentioning

confidence: 99%

Successful Fertilization in a Case of Globozoospermia Through Assisted Oocyte Activation With Calcium Ionophores: A Case Report

Thapa,

Bawaskar,

Badge

et al. 2024

Cureus

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Globozoospermia is a rare sperm morphological abnormality characterized by a lack of acrosomes and postacrosomal sheaths, defects in the cytoskeleton around the nucleus, and separated nuclear membranes. In this case, the study outlines the treatment of a 32-year-old male patient diagnosed with globozoospermia. The couple, facing primary infertility for seven years, had already undergone unsuccessful assisted reproductive technology treatments, such as two intrauterine inseminations and one in vitro fertilization. They opted for intracytoplasmic sperm injection (ICSI) with assisted oocyte activation (AOA) using a calcium (Ca) ionophore. The semen analysis showed globozoospermia, which indicated that ICSI was required for fertilization. Post-fertilization, embryo quality was assessed; three were in cleavage-stage embryos, and two grade 4AA and 3AA blastocysts and the rest were arrested at 2 pronuclear (2PN) stages, revealing successful embryo development. This case report implies that using AOA with Ca ionophores enhanced the fertilization outcomes and could be a helpful intervention strategy for patients with globozoospermia.

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A novel system for rapid conversion of Gardner embryo grades to linear scale numeric variables

Cited by 10 publications

References 32 publications

Serum Follicular Fluid Growth Differentiation Factor 9 (GDF-9) and Bone Morphogenic Protein 15 (BMP-15) as Markers of Ovarian Reserve

Serum Follicular Fluid Growth Differentiation Factor 9 (GDF-9) and Bone Morphogenic Protein 15 (BMP-15) as Markers of Ovarian Reserve

Association of Trophectoderm mRNAs and MicroRNAs with Chromosomal Aneuploidy of Embryo

Successful Fertilization in a Case of Globozoospermia Through Assisted Oocyte Activation With Calcium Ionophores: A Case Report

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